Effect of estrogen on calcium-handling proteins, β-adrenergic receptors, and function in rat heart

Sang Hui Chu, Paul Goldspink, Jill Kowalski, Jenny Beck, Dorie W. Schwertz

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Regulation of cellular Ca2+ cycling is central to myocardial contractile function. Loss of Ca2+ regulation is associated with cardiac dysfunction and pathology. Estrogen has been shown to modify contractile function and to confer cardioprotection. Therefore, we investigated the effect of estrogen on expression of rat heart myocardial Ca2+-handling proteins and beta-adrenergic receptor (β1-AR) and examined functional correlates. Female rats were sham-operated (SHAM) or ovariectomized. Two weeks after ovariectomy rats were injected (i.p.) daily with estradiol benozoate (OVX + EB) or sesame oil (OVX) for 2 weeks. Protein abundance was measured by immunoblotting and mRNA was quantified by real-time RT-PCR. OVX significantly decreased estrogen and progesterone levels and EB replacement returned both estrogen and progesterone to physiological levels. OVX induced a 75% reduction of uterine weight and a gain in body weight. Replacement restored weights to SHAM level. OVX increased and estrogen-replacement normalized abundance of β1-AR and L-type Ca2+ channel (Cav1.2) protein. OVX decreased sodium-Ca2+ exchange protein (NCX) and estrogen restored protein abundance to SHAM levels. Sarcoplasmic reticular ATPase (SERCA), phospholamban (PLB), and ryanodine receptor (RyR) abundance was not altered by hormone status. Levels of mRNA encoding for β1-AR, Cav1.2, and NCX were not influenced by OVX or estrogen replacement. OVX had no effect on SERCA and PLB mRNA level but estrogen replacement elicited a significant increase compared to OVX and SHAM. Estrogen-dependent changes in Ca2+-handling proteins and β1-AR are theoretically consistent reduced myocellular Ca2+ load. However, hormone-dependent alterations in protein were not associated with changes in contractile function.

Original languageEnglish
Pages (from-to)1257-1267
Number of pages11
JournalLife Sciences
Volume79
Issue number13
DOIs
Publication statusPublished - 2006 Aug 22

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Adrenergic Receptors
Rats
Estrogens
Calcium
Estrogen Replacement Therapy
Proteins
Messenger RNA
Progesterone
Adrenergic beta-1 Receptors
Hormones
Sesame Oil
Ryanodine Receptor Calcium Release Channel
Ovariectomy
Immunoblotting
Weight Gain
Adenosine Triphosphatases
Real-Time Polymerase Chain Reaction
Estradiol
Pathology
Sodium

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Chu, Sang Hui ; Goldspink, Paul ; Kowalski, Jill ; Beck, Jenny ; Schwertz, Dorie W. / Effect of estrogen on calcium-handling proteins, β-adrenergic receptors, and function in rat heart. In: Life Sciences. 2006 ; Vol. 79, No. 13. pp. 1257-1267.
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abstract = "Regulation of cellular Ca2+ cycling is central to myocardial contractile function. Loss of Ca2+ regulation is associated with cardiac dysfunction and pathology. Estrogen has been shown to modify contractile function and to confer cardioprotection. Therefore, we investigated the effect of estrogen on expression of rat heart myocardial Ca2+-handling proteins and beta-adrenergic receptor (β1-AR) and examined functional correlates. Female rats were sham-operated (SHAM) or ovariectomized. Two weeks after ovariectomy rats were injected (i.p.) daily with estradiol benozoate (OVX + EB) or sesame oil (OVX) for 2 weeks. Protein abundance was measured by immunoblotting and mRNA was quantified by real-time RT-PCR. OVX significantly decreased estrogen and progesterone levels and EB replacement returned both estrogen and progesterone to physiological levels. OVX induced a 75{\%} reduction of uterine weight and a gain in body weight. Replacement restored weights to SHAM level. OVX increased and estrogen-replacement normalized abundance of β1-AR and L-type Ca2+ channel (Cav1.2) protein. OVX decreased sodium-Ca2+ exchange protein (NCX) and estrogen restored protein abundance to SHAM levels. Sarcoplasmic reticular ATPase (SERCA), phospholamban (PLB), and ryanodine receptor (RyR) abundance was not altered by hormone status. Levels of mRNA encoding for β1-AR, Cav1.2, and NCX were not influenced by OVX or estrogen replacement. OVX had no effect on SERCA and PLB mRNA level but estrogen replacement elicited a significant increase compared to OVX and SHAM. Estrogen-dependent changes in Ca2+-handling proteins and β1-AR are theoretically consistent reduced myocellular Ca2+ load. However, hormone-dependent alterations in protein were not associated with changes in contractile function.",
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Effect of estrogen on calcium-handling proteins, β-adrenergic receptors, and function in rat heart. / Chu, Sang Hui; Goldspink, Paul; Kowalski, Jill; Beck, Jenny; Schwertz, Dorie W.

In: Life Sciences, Vol. 79, No. 13, 22.08.2006, p. 1257-1267.

Research output: Contribution to journalArticle

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