Effect of extracorporeal shock wave therapy on cultured tenocytes

Seung Hwan Han, jinwoo lee, Gregory P. Guyton, Brent G. Parks, Jean Paul Courneya, Lew C. Schon

Research output: Contribution to journalArticle

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Abstract

Background: Little has been reported about the biologic effect of shock waves on human normal or pathologic tendon tissue. We hypothesized that inflammatory cytokine and MMP production would be down-regulated by shock wave stimulation. Materials and Methods: Diseased Achilles tendon tissue and healthy flexor hallucis longus tissue were used. Shock wave treatment was applied to cultured cells at 0.17 mJ/inin 2energy 250, 500, 1000, and 2000 times. Results: A dose-dependent decrease in cell viability was noted in cells receiving 1000 and 2000 shocks (86.0 ± 5.6%, p = 0.01 and 72.4 ± 8.9%, p = 0.001) as compared with the normal control. Cell count in the 500-shock group increased by 23.4% as compared with the control (p = 0.05). The concentration of MMP 1, 2, and 13 was higher in diseased tenocytes as compared with normal cells (p =0.04, all comparisons). IL-6 levels were higher in the diseased tenocytes as compared with normal tenocytes (44.10 ± 16.72 versus 0.21 ± 0.55 ng/ml, (p < 0.05). IL-1 levels in normal cells increased (2.24 ± 5.02 ng/ml to 9.31 ± 6.85 ng/ml) after shock wave treatment (p = 0. 04). In diseased tenocytes, levels of MMP-1 (1.12 ± 0.23 to 0.75 ± 0.24 ng/ml; p = 0.04) and MMP-13 (1.43 ± 0.11 to 0.80 ± 0.15 ng/ml; p = 0.04) were significantly decreased after shock wave treatment. The IL-6 level in diseased tenocytes was decreased (44.10 ± 16.72 to 14.66 ± 9.49 ng/ml) after shock wave treatment (p = 0.04). Conclusion: Higher levels of MMPs and ILs were found in human tendinopathy-affected tenocytes as compared with normal cells. ESWT decreased the expression of several MMPs and ILs. Clinical Relevance: This mechanism may play an important role in shock wave treatment of tendinopathy clinically.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalFoot and Ankle International
Volume30
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

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Convulsive Therapy
Matrix Metalloproteinases
Tendinopathy
Shock
Interleukin-6
Achilles Tendon
Interleukin-1
Tendons
Tenocytes
Cultured Cells
Cell Survival
Cell Count
Cytokines

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Han, S. H., lee, J., Guyton, G. P., Parks, B. G., Courneya, J. P., & Schon, L. C. (2009). Effect of extracorporeal shock wave therapy on cultured tenocytes. Foot and Ankle International, 30(2), 93-98. https://doi.org/10.3113/FAI.2009.0093
Han, Seung Hwan ; lee, jinwoo ; Guyton, Gregory P. ; Parks, Brent G. ; Courneya, Jean Paul ; Schon, Lew C. / Effect of extracorporeal shock wave therapy on cultured tenocytes. In: Foot and Ankle International. 2009 ; Vol. 30, No. 2. pp. 93-98.
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Han, SH, lee, J, Guyton, GP, Parks, BG, Courneya, JP & Schon, LC 2009, 'Effect of extracorporeal shock wave therapy on cultured tenocytes', Foot and Ankle International, vol. 30, no. 2, pp. 93-98. https://doi.org/10.3113/FAI.2009.0093

Effect of extracorporeal shock wave therapy on cultured tenocytes. / Han, Seung Hwan; lee, jinwoo; Guyton, Gregory P.; Parks, Brent G.; Courneya, Jean Paul; Schon, Lew C.

In: Foot and Ankle International, Vol. 30, No. 2, 01.02.2009, p. 93-98.

Research output: Contribution to journalArticle

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T1 - Effect of extracorporeal shock wave therapy on cultured tenocytes

AU - Han, Seung Hwan

AU - lee, jinwoo

AU - Guyton, Gregory P.

AU - Parks, Brent G.

AU - Courneya, Jean Paul

AU - Schon, Lew C.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Background: Little has been reported about the biologic effect of shock waves on human normal or pathologic tendon tissue. We hypothesized that inflammatory cytokine and MMP production would be down-regulated by shock wave stimulation. Materials and Methods: Diseased Achilles tendon tissue and healthy flexor hallucis longus tissue were used. Shock wave treatment was applied to cultured cells at 0.17 mJ/inin 2energy 250, 500, 1000, and 2000 times. Results: A dose-dependent decrease in cell viability was noted in cells receiving 1000 and 2000 shocks (86.0 ± 5.6%, p = 0.01 and 72.4 ± 8.9%, p = 0.001) as compared with the normal control. Cell count in the 500-shock group increased by 23.4% as compared with the control (p = 0.05). The concentration of MMP 1, 2, and 13 was higher in diseased tenocytes as compared with normal cells (p =0.04, all comparisons). IL-6 levels were higher in the diseased tenocytes as compared with normal tenocytes (44.10 ± 16.72 versus 0.21 ± 0.55 ng/ml, (p < 0.05). IL-1 levels in normal cells increased (2.24 ± 5.02 ng/ml to 9.31 ± 6.85 ng/ml) after shock wave treatment (p = 0. 04). In diseased tenocytes, levels of MMP-1 (1.12 ± 0.23 to 0.75 ± 0.24 ng/ml; p = 0.04) and MMP-13 (1.43 ± 0.11 to 0.80 ± 0.15 ng/ml; p = 0.04) were significantly decreased after shock wave treatment. The IL-6 level in diseased tenocytes was decreased (44.10 ± 16.72 to 14.66 ± 9.49 ng/ml) after shock wave treatment (p = 0.04). Conclusion: Higher levels of MMPs and ILs were found in human tendinopathy-affected tenocytes as compared with normal cells. ESWT decreased the expression of several MMPs and ILs. Clinical Relevance: This mechanism may play an important role in shock wave treatment of tendinopathy clinically.

AB - Background: Little has been reported about the biologic effect of shock waves on human normal or pathologic tendon tissue. We hypothesized that inflammatory cytokine and MMP production would be down-regulated by shock wave stimulation. Materials and Methods: Diseased Achilles tendon tissue and healthy flexor hallucis longus tissue were used. Shock wave treatment was applied to cultured cells at 0.17 mJ/inin 2energy 250, 500, 1000, and 2000 times. Results: A dose-dependent decrease in cell viability was noted in cells receiving 1000 and 2000 shocks (86.0 ± 5.6%, p = 0.01 and 72.4 ± 8.9%, p = 0.001) as compared with the normal control. Cell count in the 500-shock group increased by 23.4% as compared with the control (p = 0.05). The concentration of MMP 1, 2, and 13 was higher in diseased tenocytes as compared with normal cells (p =0.04, all comparisons). IL-6 levels were higher in the diseased tenocytes as compared with normal tenocytes (44.10 ± 16.72 versus 0.21 ± 0.55 ng/ml, (p < 0.05). IL-1 levels in normal cells increased (2.24 ± 5.02 ng/ml to 9.31 ± 6.85 ng/ml) after shock wave treatment (p = 0. 04). In diseased tenocytes, levels of MMP-1 (1.12 ± 0.23 to 0.75 ± 0.24 ng/ml; p = 0.04) and MMP-13 (1.43 ± 0.11 to 0.80 ± 0.15 ng/ml; p = 0.04) were significantly decreased after shock wave treatment. The IL-6 level in diseased tenocytes was decreased (44.10 ± 16.72 to 14.66 ± 9.49 ng/ml) after shock wave treatment (p = 0.04). Conclusion: Higher levels of MMPs and ILs were found in human tendinopathy-affected tenocytes as compared with normal cells. ESWT decreased the expression of several MMPs and ILs. Clinical Relevance: This mechanism may play an important role in shock wave treatment of tendinopathy clinically.

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