Abstract
Purpose: The major pelvic ganglia (MPG) provide the majority of the innervations to the lower urinary tract. The pelvic ganglia are unique autonomic ganglia that contain both sympathetic and parasympathetic neurons. It has been known that the low-threshold voltage-gated (T-type) Ca2+ channels are only expressed only in the sympathetic neurons, whereas these channels are absent in parasympathetic neurons. In the present study, we examined the effect of fluoxetine, a world-wide used antidepressant, on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG. Materials and methods: The effect of fluoxetine on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG were examined using the whole-cell patch-clamp technique. Results: Fluoxetine inhibited the voltage-activated Ca2+ currents in the adrenergic neurons of the MPG. Both high-threshold (HVA) and low-threshold (LVA, T-type) Ca2+ currents were inhibited by fluoxetine with an IC50 of 5.3 and 10.8 μM, respectively. Fluoxetine also decreased the both the peak amplitude and the plateau of the outward K+ currents. The inhibition of the peak K+ currents by fluoxetine was concentration-dependent with an IC50 of 3.2 μM. The inhibitions of the Ca2+ and K+ currents were quickly reversible upon washout of the fluoxetine. Conclusions: These results provide evidence for the direct inhibition of the voltage dependant Ca2+ and K+ currents by fluoxetine and these inhibitory effects could modify the synaptic transmission in adrenergic neurons of the MPG.
| Original language | English |
|---|---|
| Pages (from-to) | 626-631 |
| Number of pages | 6 |
| Journal | Korean Journal of Urology |
| Volume | 46 |
| Issue number | 6 |
| Publication status | Published - 2005 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Urology
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Effect of fluoxetine on calcium or potassium channels in the neuron of rat major pelvic ganglia. / Lee, Keon Il; Song, Yun Seob; Park, Young Ho; Cha, Seung Kyu; Kim, Dae Ran; Park, Kyu Sang; Song, Eun Seop; Kong, In Deok.
In: Korean Journal of Urology, Vol. 46, No. 6, 01.06.2005, p. 626-631.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of fluoxetine on calcium or potassium channels in the neuron of rat major pelvic ganglia
AU - Lee, Keon Il
AU - Song, Yun Seob
AU - Park, Young Ho
AU - Cha, Seung Kyu
AU - Kim, Dae Ran
AU - Park, Kyu Sang
AU - Song, Eun Seop
AU - Kong, In Deok
PY - 2005/6/1
Y1 - 2005/6/1
N2 - Purpose: The major pelvic ganglia (MPG) provide the majority of the innervations to the lower urinary tract. The pelvic ganglia are unique autonomic ganglia that contain both sympathetic and parasympathetic neurons. It has been known that the low-threshold voltage-gated (T-type) Ca2+ channels are only expressed only in the sympathetic neurons, whereas these channels are absent in parasympathetic neurons. In the present study, we examined the effect of fluoxetine, a world-wide used antidepressant, on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG. Materials and methods: The effect of fluoxetine on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG were examined using the whole-cell patch-clamp technique. Results: Fluoxetine inhibited the voltage-activated Ca2+ currents in the adrenergic neurons of the MPG. Both high-threshold (HVA) and low-threshold (LVA, T-type) Ca2+ currents were inhibited by fluoxetine with an IC50 of 5.3 and 10.8 μM, respectively. Fluoxetine also decreased the both the peak amplitude and the plateau of the outward K+ currents. The inhibition of the peak K+ currents by fluoxetine was concentration-dependent with an IC50 of 3.2 μM. The inhibitions of the Ca2+ and K+ currents were quickly reversible upon washout of the fluoxetine. Conclusions: These results provide evidence for the direct inhibition of the voltage dependant Ca2+ and K+ currents by fluoxetine and these inhibitory effects could modify the synaptic transmission in adrenergic neurons of the MPG.
AB - Purpose: The major pelvic ganglia (MPG) provide the majority of the innervations to the lower urinary tract. The pelvic ganglia are unique autonomic ganglia that contain both sympathetic and parasympathetic neurons. It has been known that the low-threshold voltage-gated (T-type) Ca2+ channels are only expressed only in the sympathetic neurons, whereas these channels are absent in parasympathetic neurons. In the present study, we examined the effect of fluoxetine, a world-wide used antidepressant, on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG. Materials and methods: The effect of fluoxetine on the voltage-dependent Ca2+ and K+ currents in the adrenergic neurons of the MPG were examined using the whole-cell patch-clamp technique. Results: Fluoxetine inhibited the voltage-activated Ca2+ currents in the adrenergic neurons of the MPG. Both high-threshold (HVA) and low-threshold (LVA, T-type) Ca2+ currents were inhibited by fluoxetine with an IC50 of 5.3 and 10.8 μM, respectively. Fluoxetine also decreased the both the peak amplitude and the plateau of the outward K+ currents. The inhibition of the peak K+ currents by fluoxetine was concentration-dependent with an IC50 of 3.2 μM. The inhibitions of the Ca2+ and K+ currents were quickly reversible upon washout of the fluoxetine. Conclusions: These results provide evidence for the direct inhibition of the voltage dependant Ca2+ and K+ currents by fluoxetine and these inhibitory effects could modify the synaptic transmission in adrenergic neurons of the MPG.
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M3 - Article
AN - SCOPUS:21344442149
VL - 46
SP - 626
EP - 631
JO - Korean Journal of Urology
JF - Korean Journal of Urology
SN - 2005-6737
IS - 6
ER -