Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen

Ju Hyeong Jung; Young Bo Sim; Jong Hyun Baik; Jong Hun Park; Saint Moon Kim; Jisu Yang; Sang Hyoun Kim

doi:10.1016/j.biortech.2021.125942

Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen

Ju Hyeong Jung, Young Bo Sim, Jong Hyun Baik, Jong Hun Park, Saint Moon Kim, Jisu Yang, Sang Hyoun Kim

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

This study examined the effect of various inocula on mixed-culture dark fermentative H₂ production from food waste. Heat-treated and frozen H₂-producing granular sludge (HPG) grown with monomeric sugars showed a higher H₂ yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H₂ production performance. Propionic acid production and homoacetogenesis were regarded as major H₂-consuming pathways when methanogenic sludge was used, whereas only homoacetogenesis was found in HPG-inoculated fermentation. During fermentation, the abundance of Clostridium increased greater than 48-fold for methanogenic sludge and greater than 108-fold for HPG, respectively. The initial abundance of Clostridium showed a linear relationship with the H₂ production rate and lag-phase time. The use of inoculum with a high abundance of Clostridium is essential for H₂ production from food waste.

Original language	English
Article number	125942
Journal	Bioresource technology
Volume	342
DOIs	https://doi.org/10.1016/j.biortech.2021.125942
Publication status	Published - 2021 Dec

Bibliographical note

Funding Information:
The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF-2019M3E6A1103839 ).

Publisher Copyright:
© 2021 Elsevier Ltd

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.125942

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title = "Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen",

abstract = "This study examined the effect of various inocula on mixed-culture dark fermentative H2 production from food waste. Heat-treated and frozen H2-producing granular sludge (HPG) grown with monomeric sugars showed a higher H2 yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H2 production performance. Propionic acid production and homoacetogenesis were regarded as major H2-consuming pathways when methanogenic sludge was used, whereas only homoacetogenesis was found in HPG-inoculated fermentation. During fermentation, the abundance of Clostridium increased greater than 48-fold for methanogenic sludge and greater than 108-fold for HPG, respectively. The initial abundance of Clostridium showed a linear relationship with the H2 production rate and lag-phase time. The use of inoculum with a high abundance of Clostridium is essential for H2 production from food waste.",

author = "Jung, {Ju Hyeong} and Sim, {Young Bo} and Baik, {Jong Hyun} and Park, {Jong Hun} and Kim, {Saint Moon} and Jisu Yang and Kim, {Sang Hyoun}",

note = "Funding Information: The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF-2019M3E6A1103839 ). Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

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doi = "10.1016/j.biortech.2021.125942",

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AU - Jung, Ju Hyeong

AU - Sim, Young Bo

AU - Baik, Jong Hyun

AU - Park, Jong Hun

AU - Kim, Saint Moon

AU - Yang, Jisu

AU - Kim, Sang Hyoun

N1 - Funding Information: The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF-2019M3E6A1103839 ). Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/12

Y1 - 2021/12

N2 - This study examined the effect of various inocula on mixed-culture dark fermentative H2 production from food waste. Heat-treated and frozen H2-producing granular sludge (HPG) grown with monomeric sugars showed a higher H2 yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H2 production performance. Propionic acid production and homoacetogenesis were regarded as major H2-consuming pathways when methanogenic sludge was used, whereas only homoacetogenesis was found in HPG-inoculated fermentation. During fermentation, the abundance of Clostridium increased greater than 48-fold for methanogenic sludge and greater than 108-fold for HPG, respectively. The initial abundance of Clostridium showed a linear relationship with the H2 production rate and lag-phase time. The use of inoculum with a high abundance of Clostridium is essential for H2 production from food waste.

AB - This study examined the effect of various inocula on mixed-culture dark fermentative H2 production from food waste. Heat-treated and frozen H2-producing granular sludge (HPG) grown with monomeric sugars showed a higher H2 yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H2 production performance. Propionic acid production and homoacetogenesis were regarded as major H2-consuming pathways when methanogenic sludge was used, whereas only homoacetogenesis was found in HPG-inoculated fermentation. During fermentation, the abundance of Clostridium increased greater than 48-fold for methanogenic sludge and greater than 108-fold for HPG, respectively. The initial abundance of Clostridium showed a linear relationship with the H2 production rate and lag-phase time. The use of inoculum with a high abundance of Clostridium is essential for H2 production from food waste.

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Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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