This study examined the effect of various inocula on mixed-culture dark fermentative H2 production from food waste. Heat-treated and frozen H2-producing granular sludge (HPG) grown with monomeric sugars showed a higher H2 yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H2 production performance. Propionic acid production and homoacetogenesis were regarded as major H2-consuming pathways when methanogenic sludge was used, whereas only homoacetogenesis was found in HPG-inoculated fermentation. During fermentation, the abundance of Clostridium increased greater than 48-fold for methanogenic sludge and greater than 108-fold for HPG, respectively. The initial abundance of Clostridium showed a linear relationship with the H2 production rate and lag-phase time. The use of inoculum with a high abundance of Clostridium is essential for H2 production from food waste.
|Publication status||Published - 2021 Dec|
Bibliographical noteFunding Information:
The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF-2019M3E6A1103839 ).
© 2021 Elsevier Ltd
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal