Effect of human mesenchymal stem cells on neuronal death and memory deficits induced by trimethyltin in the rat hippocampus

Jin Kyung Oh, Sin Soo Jeun, Seok Gu Kang, Kyung Soo Kim, Insop Shim

Research output: Contribution to journalArticle

Abstract

Trimethyltin (TMT) is a potent toxicant which selectively kills cells in the central nervous system (CNS) and immune system. Within the CNS, TMT selectively destroyed neurons in the neocortex, amygdala, and olfactory tubercle, however its most striking effects were observed in the hippocampal formation. In the present study, we examined the effects of human umbilical cord blood (hUCB)-derived mesenchymal stem cells (MSCs) on TMTinduced hippocampal cell death and the impairments of learning and memory in Morris water maze in rats. The hUCB-MSCs were grafted into the hippocampus 1 week after TMT (6.0 mg/kg, i.p.)-induced neurodegeneration. We identified that hUCB-MSCs survived and were differentiated in TMT-induced rat brain by the hoechst dye analysis, bromodeoxyuridine and neuronal marker NeuN immunofluorescence. In rats exposed to TMT, the hUCBMSCs grafts improved spatial learning and memory in the water maze, suggesting that grafts can in some circumstances reduce spatial deficits on the CNS after TMT-induced neurodegeneration. The present results suggest that hUCB-MSCs grafted into the TMT-induced rat brain are capable of differentiation into neurons as well as improving spatial recognition.

Original languageEnglish
Pages (from-to)1120-1127
Number of pages8
JournalTissue Engineering and Regenerative Medicine
Volume6
Issue number12
Publication statusPublished - 2009 Sep

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering

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