Effect of humoral factors from hPDLSCs on the biologic activity of hABCs

J. C. Park, S. M. Lee, J. C. Kim, J. H. Yun, K. S. Cho, G. I. Im, B. S. Kim, ChangSung Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: The human periodontal ligament stem cells (hPDLSCs) and human alveolar bone-derived stromal cells (hABCs) seem to be closely involved in the maintenance of alveolar bone in an anatomically indirect manner; however, there is little study on this matter. Therefore, the effect of hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was evaluated, focusing on the humoral factors released by hPDLSCs. Materials and methods: Human periodontal ligament stem cells and hABCs were isolated and characterized. hPDLSCs were indirectly cocultured to observe the in vitro effect of humoral factors released from hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs. Human gingival fibroblasts (hGFs) were utilized as positive control. Results: Isolated cells demonstrated the presence of stem cells within. Indirect coculture of hPDLSCs greatly inhibited osteoclastogenesis by hABCs. Osteogenesis/adipogenesis of hABCs was also inhibited by indirect coculture with hPDLSC. The magnitude of regulatory effect from hPDLSCs was significantly greater than that of hGFs. Conclusions: Humoral factors released from hPDLSCs seemed to modulate the differentiation of hABCs, and the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was all inhibited, suggesting the potential role of hPDLSCs in the maintenance of the alveolar bone.

Original languageEnglish
Pages (from-to)537-547
Number of pages11
JournalOral Diseases
Volume18
Issue number6
DOIs
Publication statusPublished - 2012 Sep 1

Fingerprint

Periodontal Ligament
Stromal Cells
Human Activities
Stem Cells
Bone and Bones
Coculture Techniques
Osteogenesis
Fibroblasts
Maintenance

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Park, J. C., Lee, S. M., Kim, J. C., Yun, J. H., Cho, K. S., Im, G. I., ... Kim, C. (2012). Effect of humoral factors from hPDLSCs on the biologic activity of hABCs. Oral Diseases, 18(6), 537-547. https://doi.org/10.1111/j.1601-0825.2012.01906.x
Park, J. C. ; Lee, S. M. ; Kim, J. C. ; Yun, J. H. ; Cho, K. S. ; Im, G. I. ; Kim, B. S. ; Kim, ChangSung. / Effect of humoral factors from hPDLSCs on the biologic activity of hABCs. In: Oral Diseases. 2012 ; Vol. 18, No. 6. pp. 537-547.
@article{079a975de4ad4e6fafbda5e102604d57,
title = "Effect of humoral factors from hPDLSCs on the biologic activity of hABCs",
abstract = "Objective: The human periodontal ligament stem cells (hPDLSCs) and human alveolar bone-derived stromal cells (hABCs) seem to be closely involved in the maintenance of alveolar bone in an anatomically indirect manner; however, there is little study on this matter. Therefore, the effect of hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was evaluated, focusing on the humoral factors released by hPDLSCs. Materials and methods: Human periodontal ligament stem cells and hABCs were isolated and characterized. hPDLSCs were indirectly cocultured to observe the in vitro effect of humoral factors released from hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs. Human gingival fibroblasts (hGFs) were utilized as positive control. Results: Isolated cells demonstrated the presence of stem cells within. Indirect coculture of hPDLSCs greatly inhibited osteoclastogenesis by hABCs. Osteogenesis/adipogenesis of hABCs was also inhibited by indirect coculture with hPDLSC. The magnitude of regulatory effect from hPDLSCs was significantly greater than that of hGFs. Conclusions: Humoral factors released from hPDLSCs seemed to modulate the differentiation of hABCs, and the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was all inhibited, suggesting the potential role of hPDLSCs in the maintenance of the alveolar bone.",
author = "Park, {J. C.} and Lee, {S. M.} and Kim, {J. C.} and Yun, {J. H.} and Cho, {K. S.} and Im, {G. I.} and Kim, {B. S.} and ChangSung Kim",
year = "2012",
month = "9",
day = "1",
doi = "10.1111/j.1601-0825.2012.01906.x",
language = "English",
volume = "18",
pages = "537--547",
journal = "Oral Diseases",
issn = "1354-523X",
publisher = "Wiley-Blackwell",
number = "6",

}

Park, JC, Lee, SM, Kim, JC, Yun, JH, Cho, KS, Im, GI, Kim, BS & Kim, C 2012, 'Effect of humoral factors from hPDLSCs on the biologic activity of hABCs', Oral Diseases, vol. 18, no. 6, pp. 537-547. https://doi.org/10.1111/j.1601-0825.2012.01906.x

Effect of humoral factors from hPDLSCs on the biologic activity of hABCs. / Park, J. C.; Lee, S. M.; Kim, J. C.; Yun, J. H.; Cho, K. S.; Im, G. I.; Kim, B. S.; Kim, ChangSung.

In: Oral Diseases, Vol. 18, No. 6, 01.09.2012, p. 537-547.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of humoral factors from hPDLSCs on the biologic activity of hABCs

AU - Park, J. C.

AU - Lee, S. M.

AU - Kim, J. C.

AU - Yun, J. H.

AU - Cho, K. S.

AU - Im, G. I.

AU - Kim, B. S.

AU - Kim, ChangSung

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Objective: The human periodontal ligament stem cells (hPDLSCs) and human alveolar bone-derived stromal cells (hABCs) seem to be closely involved in the maintenance of alveolar bone in an anatomically indirect manner; however, there is little study on this matter. Therefore, the effect of hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was evaluated, focusing on the humoral factors released by hPDLSCs. Materials and methods: Human periodontal ligament stem cells and hABCs were isolated and characterized. hPDLSCs were indirectly cocultured to observe the in vitro effect of humoral factors released from hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs. Human gingival fibroblasts (hGFs) were utilized as positive control. Results: Isolated cells demonstrated the presence of stem cells within. Indirect coculture of hPDLSCs greatly inhibited osteoclastogenesis by hABCs. Osteogenesis/adipogenesis of hABCs was also inhibited by indirect coculture with hPDLSC. The magnitude of regulatory effect from hPDLSCs was significantly greater than that of hGFs. Conclusions: Humoral factors released from hPDLSCs seemed to modulate the differentiation of hABCs, and the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was all inhibited, suggesting the potential role of hPDLSCs in the maintenance of the alveolar bone.

AB - Objective: The human periodontal ligament stem cells (hPDLSCs) and human alveolar bone-derived stromal cells (hABCs) seem to be closely involved in the maintenance of alveolar bone in an anatomically indirect manner; however, there is little study on this matter. Therefore, the effect of hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was evaluated, focusing on the humoral factors released by hPDLSCs. Materials and methods: Human periodontal ligament stem cells and hABCs were isolated and characterized. hPDLSCs were indirectly cocultured to observe the in vitro effect of humoral factors released from hPDLSCs on the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs. Human gingival fibroblasts (hGFs) were utilized as positive control. Results: Isolated cells demonstrated the presence of stem cells within. Indirect coculture of hPDLSCs greatly inhibited osteoclastogenesis by hABCs. Osteogenesis/adipogenesis of hABCs was also inhibited by indirect coculture with hPDLSC. The magnitude of regulatory effect from hPDLSCs was significantly greater than that of hGFs. Conclusions: Humoral factors released from hPDLSCs seemed to modulate the differentiation of hABCs, and the osteoclastogenic, osteogenic, and adipogenic differentiation of hABCs was all inhibited, suggesting the potential role of hPDLSCs in the maintenance of the alveolar bone.

UR - http://www.scopus.com/inward/record.url?scp=84864835562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864835562&partnerID=8YFLogxK

U2 - 10.1111/j.1601-0825.2012.01906.x

DO - 10.1111/j.1601-0825.2012.01906.x

M3 - Article

VL - 18

SP - 537

EP - 547

JO - Oral Diseases

JF - Oral Diseases

SN - 1354-523X

IS - 6

ER -