Effect of hypo-, iso- and hypertonic saline irrigation on secretory mucins and morphology of cultured human nasal epithelial cells

Chang Hoon Kim, Mee Hyun Song, Young Eun Ahn, Jeung Gweon Lee, Joo Heon Yoon

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Conclusion. We speculate that isotonic saline is the most physiological irrigation solution in terms of mucus secretion and the cellular morphology of nasal epithelial cells. Objective. To determine the most physiologic and effective saline concentration for nasal irrigation in terms of mucus secretion and cellular morphology by using normal human nasal epithelial cells treated with hypo-, iso- and hypertonic saline. Material and methods. Fully differentiated passage-2 normal human nasal epithelial cells were treated with pure water and with 0.3% (hypotonic), 0.9% (isotonic) and 3% (hypertonic) saline solutions. mRNA expression levels of MUC5AC and MUC5B, which are known to be major airway mucins, were analyzed after 30 min using reverse transcriptase polymerase chain reaction. Total mucin and MUC5AC and MUC5B mucin secretions were analyzed using dot-blotting. Cellular morphology was observed with light microscopy after hematoxylin-eosin staining and with scanning electron microscopy. Results. MUC5AC and MUC5B mRNA levels did not change after treatment with pure water and various concentrations of saline. Total mucin and MUC5AC mucin secretions only increased following pure water treatment, while MUC5B mucin secretion increased with pure water, hypo- and hypertonic saline treatment. Morphologic analysis revealed that pure water severely damaged normal human nasal epithelial cells and that only isotonic saline did not affect their morphology.

Original languageEnglish
Pages (from-to)1296-1300
Number of pages5
JournalActa Oto-Laryngologica
Volume125
Issue number12
DOIs
Publication statusPublished - 2005 Dec

Bibliographical note

Funding Information:
This study was supported by a faculty research grant (No. 6-2004-92) from Yonsei University College of Medicine for 2004.

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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