Effect of intercalating dyes on the production of antibiotics by Micromonospora rosaria and Micromonospora purpurea

K. S. Kim, Hyunsook Pai, S. Y. Lee, Dewey D.Y. Ryu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing ability appeared to be strain-dependent. In M. purpurea, up to 90% of colonies were found to have lost gentamicin-producing ability when protoplasts were used in the test. These antibiotic-nonproducing strains were further studied. The following observations were made: (1) Unlike the producing ability, the resistance to the antibiotics is a very stable character in both species. (2) Protoplast fusion analysis indicates that rosamicin-nonproducing characteristics of MR 217-AF2 and MR 217-AF3 strains induced by the acriflavine treatment is due to chromosomal mutation or rearrangement but not to loss of a plasmid. (3) Gentamicin-nonproducing strains of M. purpurea responded differently to the supplementation of streptamine or DOS in the culture medium. When supplemented with streptamine or DOS, some of these strains regained the ability to produce antibiotic, showing that the biosynthesis of intermediate was affected in these strains.

Original languageEnglish
Pages (from-to)564-570
Number of pages7
JournalEnzyme and Microbial Technology
Volume12
Issue number8
DOIs
Publication statusPublished - 1990 Jan 1

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Micromonospora
Antibiotics
Coloring Agents
Dyes
Anti-Bacterial Agents
Acriflavine
Protoplasts
DOS
Gentamicins
Microbial Drug Resistance
Biosynthesis
Culture Media
Plasmids
Fusion reactions
Productivity
Mutation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

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title = "Effect of intercalating dyes on the production of antibiotics by Micromonospora rosaria and Micromonospora purpurea",
abstract = "The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing ability appeared to be strain-dependent. In M. purpurea, up to 90{\%} of colonies were found to have lost gentamicin-producing ability when protoplasts were used in the test. These antibiotic-nonproducing strains were further studied. The following observations were made: (1) Unlike the producing ability, the resistance to the antibiotics is a very stable character in both species. (2) Protoplast fusion analysis indicates that rosamicin-nonproducing characteristics of MR 217-AF2 and MR 217-AF3 strains induced by the acriflavine treatment is due to chromosomal mutation or rearrangement but not to loss of a plasmid. (3) Gentamicin-nonproducing strains of M. purpurea responded differently to the supplementation of streptamine or DOS in the culture medium. When supplemented with streptamine or DOS, some of these strains regained the ability to produce antibiotic, showing that the biosynthesis of intermediate was affected in these strains.",
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Effect of intercalating dyes on the production of antibiotics by Micromonospora rosaria and Micromonospora purpurea. / Kim, K. S.; Pai, Hyunsook; Lee, S. Y.; Ryu, Dewey D.Y.

In: Enzyme and Microbial Technology, Vol. 12, No. 8, 01.01.1990, p. 564-570.

Research output: Contribution to journalArticle

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