Effect of iron on adherence and cytotoxicity of Entamoeba histolytica to CHO cell monolayers

Jongweon Lee, Soon Jung Park, Taisoon Yong

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Iron is an essential element for almost all living organisms. The possible role of iron for growth, adherence and cytotoxicity of Entamoeba histolytica was evaluated in this study. The absence of iron from TYI-S-33 medium stopped amebic growth in vitro. However, iron concentrations in the culture media of 21.4-285.6 μM did not affect the growth of the amebae. Although growth was not retarded at these concentrations, the adhesive abilities of E. histolytica and their cytotoxicities to CHO cell monolayer were correlated with iron concentration. Amebic adhesion to CHO cell monolayers was significantly reduced by low-iron (24.6 ± 2.1%) compared with 62.7 ± 2.8 and 63.1 ± 1.4% of amebae grown in a normal-iron and high-iron media, respectively. E. histolytica cultured in the normal- and high-iron media destroyed 69.1 ± 4.3% and 72.6 ± 5.7% of cultured CHO cell monolayers, but amebae grown in the low-iron medium showed a significantly reduced level of cytotoxicity to CHO cells (2.8 ± 0.2%). Addition of divalent cations other than iron to amebic trophozoites grown in the low-iron medium failed to restore levels of the cytotoxicity. However, when E. histolytica grown in low-iron medium were transferred to normal-iron medium, the amebae showed completely restored cytotoxicity within 7 days. The result suggests that iron is an important factor in the adherence and cytotoxicity of E. histolytica to CHO cell monolayer.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalKorean Journal of Parasitology
Volume46
Issue number1
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Entamoeba histolytica
CHO Cells
Iron
Amoeba
Growth
Trophozoites
Divalent Cations
Adhesives

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

@article{85c36ff30f274fbe868aebcd9c0ed05f,
title = "Effect of iron on adherence and cytotoxicity of Entamoeba histolytica to CHO cell monolayers",
abstract = "Iron is an essential element for almost all living organisms. The possible role of iron for growth, adherence and cytotoxicity of Entamoeba histolytica was evaluated in this study. The absence of iron from TYI-S-33 medium stopped amebic growth in vitro. However, iron concentrations in the culture media of 21.4-285.6 μM did not affect the growth of the amebae. Although growth was not retarded at these concentrations, the adhesive abilities of E. histolytica and their cytotoxicities to CHO cell monolayer were correlated with iron concentration. Amebic adhesion to CHO cell monolayers was significantly reduced by low-iron (24.6 ± 2.1{\%}) compared with 62.7 ± 2.8 and 63.1 ± 1.4{\%} of amebae grown in a normal-iron and high-iron media, respectively. E. histolytica cultured in the normal- and high-iron media destroyed 69.1 ± 4.3{\%} and 72.6 ± 5.7{\%} of cultured CHO cell monolayers, but amebae grown in the low-iron medium showed a significantly reduced level of cytotoxicity to CHO cells (2.8 ± 0.2{\%}). Addition of divalent cations other than iron to amebic trophozoites grown in the low-iron medium failed to restore levels of the cytotoxicity. However, when E. histolytica grown in low-iron medium were transferred to normal-iron medium, the amebae showed completely restored cytotoxicity within 7 days. The result suggests that iron is an important factor in the adherence and cytotoxicity of E. histolytica to CHO cell monolayer.",
author = "Jongweon Lee and Park, {Soon Jung} and Taisoon Yong",
year = "2008",
month = "12",
day = "1",
doi = "10.3347/kjp.2008.46.1.37",
language = "English",
volume = "46",
pages = "37--40",
journal = "Korean Journal of Parasitology",
issn = "0023-4001",
publisher = "Korean Journal of Parasitology",
number = "1",

}

Effect of iron on adherence and cytotoxicity of Entamoeba histolytica to CHO cell monolayers. / Lee, Jongweon; Park, Soon Jung; Yong, Taisoon.

In: Korean Journal of Parasitology, Vol. 46, No. 1, 01.12.2008, p. 37-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of iron on adherence and cytotoxicity of Entamoeba histolytica to CHO cell monolayers

AU - Lee, Jongweon

AU - Park, Soon Jung

AU - Yong, Taisoon

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Iron is an essential element for almost all living organisms. The possible role of iron for growth, adherence and cytotoxicity of Entamoeba histolytica was evaluated in this study. The absence of iron from TYI-S-33 medium stopped amebic growth in vitro. However, iron concentrations in the culture media of 21.4-285.6 μM did not affect the growth of the amebae. Although growth was not retarded at these concentrations, the adhesive abilities of E. histolytica and their cytotoxicities to CHO cell monolayer were correlated with iron concentration. Amebic adhesion to CHO cell monolayers was significantly reduced by low-iron (24.6 ± 2.1%) compared with 62.7 ± 2.8 and 63.1 ± 1.4% of amebae grown in a normal-iron and high-iron media, respectively. E. histolytica cultured in the normal- and high-iron media destroyed 69.1 ± 4.3% and 72.6 ± 5.7% of cultured CHO cell monolayers, but amebae grown in the low-iron medium showed a significantly reduced level of cytotoxicity to CHO cells (2.8 ± 0.2%). Addition of divalent cations other than iron to amebic trophozoites grown in the low-iron medium failed to restore levels of the cytotoxicity. However, when E. histolytica grown in low-iron medium were transferred to normal-iron medium, the amebae showed completely restored cytotoxicity within 7 days. The result suggests that iron is an important factor in the adherence and cytotoxicity of E. histolytica to CHO cell monolayer.

AB - Iron is an essential element for almost all living organisms. The possible role of iron for growth, adherence and cytotoxicity of Entamoeba histolytica was evaluated in this study. The absence of iron from TYI-S-33 medium stopped amebic growth in vitro. However, iron concentrations in the culture media of 21.4-285.6 μM did not affect the growth of the amebae. Although growth was not retarded at these concentrations, the adhesive abilities of E. histolytica and their cytotoxicities to CHO cell monolayer were correlated with iron concentration. Amebic adhesion to CHO cell monolayers was significantly reduced by low-iron (24.6 ± 2.1%) compared with 62.7 ± 2.8 and 63.1 ± 1.4% of amebae grown in a normal-iron and high-iron media, respectively. E. histolytica cultured in the normal- and high-iron media destroyed 69.1 ± 4.3% and 72.6 ± 5.7% of cultured CHO cell monolayers, but amebae grown in the low-iron medium showed a significantly reduced level of cytotoxicity to CHO cells (2.8 ± 0.2%). Addition of divalent cations other than iron to amebic trophozoites grown in the low-iron medium failed to restore levels of the cytotoxicity. However, when E. histolytica grown in low-iron medium were transferred to normal-iron medium, the amebae showed completely restored cytotoxicity within 7 days. The result suggests that iron is an important factor in the adherence and cytotoxicity of E. histolytica to CHO cell monolayer.

UR - http://www.scopus.com/inward/record.url?scp=52049123874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049123874&partnerID=8YFLogxK

U2 - 10.3347/kjp.2008.46.1.37

DO - 10.3347/kjp.2008.46.1.37

M3 - Article

VL - 46

SP - 37

EP - 40

JO - Korean Journal of Parasitology

JF - Korean Journal of Parasitology

SN - 0023-4001

IS - 1

ER -