Effect of Lactobacillus GG and conditioned media on IL-1β-induced IL-8 production in Caco-2 cells

Chang Hwan Choi, Tae Il Kim, SangKil Lee, Kyung Min Yang, Won Ho Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective. To evaluate the anti-inflammatory effect of Lactobacillus casei rhamnosus GG (LGG) and its conditioned media (CM) in stimulated Caco-2 cells and to characterize the components of LGG that have the anti-inflammatory effect. Material and methods. Caco-2 cells were stimulated with IL-1β with or without LGG or LGG-CM. Production of IL-8 was measured by enzyme-linked immunosorbent assay (ELISA). The transcriptional activities of the IL-8 gene and the NF-κB-responsive gene were evaluated by a transient transfection of the luciferase reporter gene. The effect on IκBα degradation was evaluated by Western blot analysis. To determine the nature of the immunomodulatory molecules, the LGG was modified to the following: treated with antibiotics, 4% formaldehyde, incubation at 95°C, or sonication. Results. We demonstrated that the pretreatment of Caco-2 cells with LGG significantly inhibited IL-1β-induced IL-8 production. Furthermore, LGG attenuated the IL-1β-induced transcriptional activation of the IL-8 gene and the NF-κB-responsive gene, and attenuated the IL-1β-induced IκBα degradation. Formaldehyde-fixed or antibiotics-treated LGG maintained the inhibitory effect, but heated LGG lost this effect. Sonicated LGG debris had a similar inhibitory effect with whole bacterial cells. LGG-CM attenuated IL-1β-induced IL-8 production. This effect was maintained even when the conditioned media were heated. Conclusions. LGG inhibited IL-1β-induced IL-8 production in Caco-2 and this effect occurred at the transcriptional level, at least in part, by inhibition of the NF-κB signaling pathway. Both the structural material of LGG and the soluble factor secreted from LGG inhibited the IL-1β-induced IL-8 production, and thus different substances may cause the effects.

Original languageEnglish
Pages (from-to)938-947
Number of pages10
JournalScandinavian Journal of Gastroenterology
Volume43
Issue number8
DOIs
Publication statusPublished - 2008 Jul 30

Fingerprint

Lactobacillus rhamnosus
Caco-2 Cells
Conditioned Culture Medium
Interleukin-8
Interleukin-1
Formaldehyde
Genes
Anti-Inflammatory Agents
Anti-Bacterial Agents
Lactobacillus casei
Sonication
Luciferases
Reporter Genes
Transcriptional Activation
Transfection
Western Blotting
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Hwan Choi, Chang ; Il Kim, Tae ; Lee, SangKil ; Min Yang, Kyung ; Ho Kim, Won. / Effect of Lactobacillus GG and conditioned media on IL-1β-induced IL-8 production in Caco-2 cells. In: Scandinavian Journal of Gastroenterology. 2008 ; Vol. 43, No. 8. pp. 938-947.
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title = "Effect of Lactobacillus GG and conditioned media on IL-1β-induced IL-8 production in Caco-2 cells",
abstract = "Objective. To evaluate the anti-inflammatory effect of Lactobacillus casei rhamnosus GG (LGG) and its conditioned media (CM) in stimulated Caco-2 cells and to characterize the components of LGG that have the anti-inflammatory effect. Material and methods. Caco-2 cells were stimulated with IL-1β with or without LGG or LGG-CM. Production of IL-8 was measured by enzyme-linked immunosorbent assay (ELISA). The transcriptional activities of the IL-8 gene and the NF-κB-responsive gene were evaluated by a transient transfection of the luciferase reporter gene. The effect on IκBα degradation was evaluated by Western blot analysis. To determine the nature of the immunomodulatory molecules, the LGG was modified to the following: treated with antibiotics, 4{\%} formaldehyde, incubation at 95°C, or sonication. Results. We demonstrated that the pretreatment of Caco-2 cells with LGG significantly inhibited IL-1β-induced IL-8 production. Furthermore, LGG attenuated the IL-1β-induced transcriptional activation of the IL-8 gene and the NF-κB-responsive gene, and attenuated the IL-1β-induced IκBα degradation. Formaldehyde-fixed or antibiotics-treated LGG maintained the inhibitory effect, but heated LGG lost this effect. Sonicated LGG debris had a similar inhibitory effect with whole bacterial cells. LGG-CM attenuated IL-1β-induced IL-8 production. This effect was maintained even when the conditioned media were heated. Conclusions. LGG inhibited IL-1β-induced IL-8 production in Caco-2 and this effect occurred at the transcriptional level, at least in part, by inhibition of the NF-κB signaling pathway. Both the structural material of LGG and the soluble factor secreted from LGG inhibited the IL-1β-induced IL-8 production, and thus different substances may cause the effects.",
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Effect of Lactobacillus GG and conditioned media on IL-1β-induced IL-8 production in Caco-2 cells. / Hwan Choi, Chang; Il Kim, Tae; Lee, SangKil; Min Yang, Kyung; Ho Kim, Won.

In: Scandinavian Journal of Gastroenterology, Vol. 43, No. 8, 30.07.2008, p. 938-947.

Research output: Contribution to journalArticle

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T1 - Effect of Lactobacillus GG and conditioned media on IL-1β-induced IL-8 production in Caco-2 cells

AU - Hwan Choi, Chang

AU - Il Kim, Tae

AU - Lee, SangKil

AU - Min Yang, Kyung

AU - Ho Kim, Won

PY - 2008/7/30

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N2 - Objective. To evaluate the anti-inflammatory effect of Lactobacillus casei rhamnosus GG (LGG) and its conditioned media (CM) in stimulated Caco-2 cells and to characterize the components of LGG that have the anti-inflammatory effect. Material and methods. Caco-2 cells were stimulated with IL-1β with or without LGG or LGG-CM. Production of IL-8 was measured by enzyme-linked immunosorbent assay (ELISA). The transcriptional activities of the IL-8 gene and the NF-κB-responsive gene were evaluated by a transient transfection of the luciferase reporter gene. The effect on IκBα degradation was evaluated by Western blot analysis. To determine the nature of the immunomodulatory molecules, the LGG was modified to the following: treated with antibiotics, 4% formaldehyde, incubation at 95°C, or sonication. Results. We demonstrated that the pretreatment of Caco-2 cells with LGG significantly inhibited IL-1β-induced IL-8 production. Furthermore, LGG attenuated the IL-1β-induced transcriptional activation of the IL-8 gene and the NF-κB-responsive gene, and attenuated the IL-1β-induced IκBα degradation. Formaldehyde-fixed or antibiotics-treated LGG maintained the inhibitory effect, but heated LGG lost this effect. Sonicated LGG debris had a similar inhibitory effect with whole bacterial cells. LGG-CM attenuated IL-1β-induced IL-8 production. This effect was maintained even when the conditioned media were heated. Conclusions. LGG inhibited IL-1β-induced IL-8 production in Caco-2 and this effect occurred at the transcriptional level, at least in part, by inhibition of the NF-κB signaling pathway. Both the structural material of LGG and the soluble factor secreted from LGG inhibited the IL-1β-induced IL-8 production, and thus different substances may cause the effects.

AB - Objective. To evaluate the anti-inflammatory effect of Lactobacillus casei rhamnosus GG (LGG) and its conditioned media (CM) in stimulated Caco-2 cells and to characterize the components of LGG that have the anti-inflammatory effect. Material and methods. Caco-2 cells were stimulated with IL-1β with or without LGG or LGG-CM. Production of IL-8 was measured by enzyme-linked immunosorbent assay (ELISA). The transcriptional activities of the IL-8 gene and the NF-κB-responsive gene were evaluated by a transient transfection of the luciferase reporter gene. The effect on IκBα degradation was evaluated by Western blot analysis. To determine the nature of the immunomodulatory molecules, the LGG was modified to the following: treated with antibiotics, 4% formaldehyde, incubation at 95°C, or sonication. Results. We demonstrated that the pretreatment of Caco-2 cells with LGG significantly inhibited IL-1β-induced IL-8 production. Furthermore, LGG attenuated the IL-1β-induced transcriptional activation of the IL-8 gene and the NF-κB-responsive gene, and attenuated the IL-1β-induced IκBα degradation. Formaldehyde-fixed or antibiotics-treated LGG maintained the inhibitory effect, but heated LGG lost this effect. Sonicated LGG debris had a similar inhibitory effect with whole bacterial cells. LGG-CM attenuated IL-1β-induced IL-8 production. This effect was maintained even when the conditioned media were heated. Conclusions. LGG inhibited IL-1β-induced IL-8 production in Caco-2 and this effect occurred at the transcriptional level, at least in part, by inhibition of the NF-κB signaling pathway. Both the structural material of LGG and the soluble factor secreted from LGG inhibited the IL-1β-induced IL-8 production, and thus different substances may cause the effects.

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