Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts

Dong Woon Lee, Jae Gu Kim, Meyoung Kon Kim, Sahar Ansari, Alireza Moshaverinia, Seong Ho Choi, Jae Jun Ryu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-μm dimples at 10-μm intervals, and SM30 (n=16) had 5-μm dimples at 25-μm intervals in a 2 × 4 mm2 area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-μm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

Original languageEnglish
Pages (from-to)138-145
Number of pages8
JournalJournal of Advanced Prosthodontics
Volume7
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1

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Titanium
Lasers
Fibroblasts
Epithelial Cells
Numismatics
Squamous Cell Carcinoma
Proteins
Cell Proliferation
Staining and Labeling
Lung

All Science Journal Classification (ASJC) codes

  • Oral Surgery
  • Dentistry (miscellaneous)

Cite this

Lee, Dong Woon ; Kim, Jae Gu ; Kim, Meyoung Kon ; Ansari, Sahar ; Moshaverinia, Alireza ; Choi, Seong Ho ; Ryu, Jae Jun. / Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts. In: Journal of Advanced Prosthodontics. 2015 ; Vol. 7, No. 2. pp. 138-145.
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Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts. / Lee, Dong Woon; Kim, Jae Gu; Kim, Meyoung Kon; Ansari, Sahar; Moshaverinia, Alireza; Choi, Seong Ho; Ryu, Jae Jun.

In: Journal of Advanced Prosthodontics, Vol. 7, No. 2, 01.01.2015, p. 138-145.

Research output: Contribution to journalArticle

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AU - Lee, Dong Woon

AU - Kim, Jae Gu

AU - Kim, Meyoung Kon

AU - Ansari, Sahar

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AU - Choi, Seong Ho

AU - Ryu, Jae Jun

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N2 - Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti. Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-μm dimples at 10-μm intervals, and SM30 (n=16) had 5-μm dimples at 25-μm intervals in a 2 × 4 mm2 area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-μm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

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