Effect of major histocompatibility complex haplotype matching by C4 and MICA genotyping on acute graft versus host disease in unrelated hematopoietic stem cell transplantation

Yongjung Park, June Won Cheong, Myoung Hee Park, Myoung Soo Kim, Jong Sun Kim, Hyonsuk Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We explored whether matching of human leukocyte antigen (HLA) haplotypes between the recipient and donor of hematopoietic stem cell transplantation (HSCT) predicted by C4 and MICA typing is associated with the incidence of acute graft versus host disease (aGVHD). DNA preparations collected from a total of 81 recipient and donor pairs were used for PCR-based C4 subtyping and/or MICA sequence-based typing. Incidences of aGVHD were compared according to C4 and MICA matching. The six most common MICA alleles were MICA*008:01, *010:01, *002:01, *004, *009:01/049, and *012:01. Among the 59 unrelated pairs, HLA alleles were matched in 34 (57.6%). C4 subtypes were identical between the recipient and donor in 28 (82.4%) HLA-matched unrelated pairs, while MICA genotypes were matched in all HLA-matched unrelated pairs. In the 22 HLA-matched related pairs, all recipients showed identical C4 subtypes with their respective donors. In multivariate analysis, C4 mismatch was a significant risk factor associated with the development of aGVHD in unrelated HSCT (hazard ratio = 3.24, P = 0.006). PCR-based C4 subtyping is a simple method for assessing the genetic identity of the HLA region between a recipient and unrelated donor. This test would be also useful for prediction of aGVHD in HSCT.

Original languageEnglish
Pages (from-to)176-183
Number of pages8
JournalHuman Immunology
Volume77
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Hematopoietic Stem Cell Transplantation
Graft vs Host Disease
HLA Antigens
Major Histocompatibility Complex
Haplotypes
Tissue Donors
Alleles
Polymerase Chain Reaction
Unrelated Donors
Incidence
Pedigree
Multivariate Analysis
Genotype
DNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

@article{d3c14995736849548d204f009f188e16,
title = "Effect of major histocompatibility complex haplotype matching by C4 and MICA genotyping on acute graft versus host disease in unrelated hematopoietic stem cell transplantation",
abstract = "We explored whether matching of human leukocyte antigen (HLA) haplotypes between the recipient and donor of hematopoietic stem cell transplantation (HSCT) predicted by C4 and MICA typing is associated with the incidence of acute graft versus host disease (aGVHD). DNA preparations collected from a total of 81 recipient and donor pairs were used for PCR-based C4 subtyping and/or MICA sequence-based typing. Incidences of aGVHD were compared according to C4 and MICA matching. The six most common MICA alleles were MICA*008:01, *010:01, *002:01, *004, *009:01/049, and *012:01. Among the 59 unrelated pairs, HLA alleles were matched in 34 (57.6{\%}). C4 subtypes were identical between the recipient and donor in 28 (82.4{\%}) HLA-matched unrelated pairs, while MICA genotypes were matched in all HLA-matched unrelated pairs. In the 22 HLA-matched related pairs, all recipients showed identical C4 subtypes with their respective donors. In multivariate analysis, C4 mismatch was a significant risk factor associated with the development of aGVHD in unrelated HSCT (hazard ratio = 3.24, P = 0.006). PCR-based C4 subtyping is a simple method for assessing the genetic identity of the HLA region between a recipient and unrelated donor. This test would be also useful for prediction of aGVHD in HSCT.",
author = "Yongjung Park and Cheong, {June Won} and Park, {Myoung Hee} and Kim, {Myoung Soo} and Kim, {Jong Sun} and Hyonsuk Kim",
year = "2016",
month = "2",
day = "1",
doi = "10.1016/j.humimm.2015.11.015",
language = "English",
volume = "77",
pages = "176--183",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "2",

}

Effect of major histocompatibility complex haplotype matching by C4 and MICA genotyping on acute graft versus host disease in unrelated hematopoietic stem cell transplantation. / Park, Yongjung; Cheong, June Won; Park, Myoung Hee; Kim, Myoung Soo; Kim, Jong Sun; Kim, Hyonsuk.

In: Human Immunology, Vol. 77, No. 2, 01.02.2016, p. 176-183.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of major histocompatibility complex haplotype matching by C4 and MICA genotyping on acute graft versus host disease in unrelated hematopoietic stem cell transplantation

AU - Park, Yongjung

AU - Cheong, June Won

AU - Park, Myoung Hee

AU - Kim, Myoung Soo

AU - Kim, Jong Sun

AU - Kim, Hyonsuk

PY - 2016/2/1

Y1 - 2016/2/1

N2 - We explored whether matching of human leukocyte antigen (HLA) haplotypes between the recipient and donor of hematopoietic stem cell transplantation (HSCT) predicted by C4 and MICA typing is associated with the incidence of acute graft versus host disease (aGVHD). DNA preparations collected from a total of 81 recipient and donor pairs were used for PCR-based C4 subtyping and/or MICA sequence-based typing. Incidences of aGVHD were compared according to C4 and MICA matching. The six most common MICA alleles were MICA*008:01, *010:01, *002:01, *004, *009:01/049, and *012:01. Among the 59 unrelated pairs, HLA alleles were matched in 34 (57.6%). C4 subtypes were identical between the recipient and donor in 28 (82.4%) HLA-matched unrelated pairs, while MICA genotypes were matched in all HLA-matched unrelated pairs. In the 22 HLA-matched related pairs, all recipients showed identical C4 subtypes with their respective donors. In multivariate analysis, C4 mismatch was a significant risk factor associated with the development of aGVHD in unrelated HSCT (hazard ratio = 3.24, P = 0.006). PCR-based C4 subtyping is a simple method for assessing the genetic identity of the HLA region between a recipient and unrelated donor. This test would be also useful for prediction of aGVHD in HSCT.

AB - We explored whether matching of human leukocyte antigen (HLA) haplotypes between the recipient and donor of hematopoietic stem cell transplantation (HSCT) predicted by C4 and MICA typing is associated with the incidence of acute graft versus host disease (aGVHD). DNA preparations collected from a total of 81 recipient and donor pairs were used for PCR-based C4 subtyping and/or MICA sequence-based typing. Incidences of aGVHD were compared according to C4 and MICA matching. The six most common MICA alleles were MICA*008:01, *010:01, *002:01, *004, *009:01/049, and *012:01. Among the 59 unrelated pairs, HLA alleles were matched in 34 (57.6%). C4 subtypes were identical between the recipient and donor in 28 (82.4%) HLA-matched unrelated pairs, while MICA genotypes were matched in all HLA-matched unrelated pairs. In the 22 HLA-matched related pairs, all recipients showed identical C4 subtypes with their respective donors. In multivariate analysis, C4 mismatch was a significant risk factor associated with the development of aGVHD in unrelated HSCT (hazard ratio = 3.24, P = 0.006). PCR-based C4 subtyping is a simple method for assessing the genetic identity of the HLA region between a recipient and unrelated donor. This test would be also useful for prediction of aGVHD in HSCT.

UR - http://www.scopus.com/inward/record.url?scp=84958124323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958124323&partnerID=8YFLogxK

U2 - 10.1016/j.humimm.2015.11.015

DO - 10.1016/j.humimm.2015.11.015

M3 - Article

C2 - 26602146

AN - SCOPUS:84958124323

VL - 77

SP - 176

EP - 183

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 2

ER -