Abstract
Type I keratins K18 and K19 undergo caspase-mediated degradation during apoptosis. Two known K18 caspase cleavage sites are aspartates in the consensus sequences VEVDA and DALDS, located within the rod domain and tail domain, respectively. Several K14 (another type I keratin) mutations within the caspase cleavage motif have been described in patients with epidermolysis bullosa simplex. Here we use extensive mutational analysis to show that K19 and K14 are caspase substrates and that the ability to undergo caspase-mediated digestion of K18, K19, or K14 is highly dependent on the location and nature of the mutation within the caspase cleavage motif. Caspase cleavage of K14 occurs at the aspartate of VEMDA, a consensus sequence found in type I keratins K12-17 with similar but not identical sequences in K18 and K19. For K18, apoptosis-induced cleavage occurs sequentially, first at 393DALD and then at 234VEVD. Hyperphosphorylation of K18 protects from caspase-3 in vitro digestion at 234VEVD but not at 393DALD. Hence, keratins K12-17 are likely caspase substrates during apoptosis. Keratin hyperphosphorylation, which occurs early in apoptosis, protects from caspase-mediated K18 digestion in a cleavage site-specific manner. Mutations in epidermolysis bullosa simplex patients could interfere with K14 degradation during apoptosis, depending on their location.
| Original language | English |
|---|---|
| Pages (from-to) | 26792-26798 |
| Number of pages | 7 |
| Journal | Journal of Biological Chemistry |
| Volume | 276 |
| Issue number | 29 |
| DOIs | |
| Publication status | Published - 2001 Jul 20 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
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Effect of Mutation and Phosphorylation of Type I Keratins on Their Caspase-mediated Degradation. / Ku, Nam-on; Omary, M. Bishr.
In: Journal of Biological Chemistry, Vol. 276, No. 29, 20.07.2001, p. 26792-26798.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of Mutation and Phosphorylation of Type I Keratins on Their Caspase-mediated Degradation
AU - Ku, Nam-on
AU - Omary, M. Bishr
PY - 2001/7/20
Y1 - 2001/7/20
N2 - Type I keratins K18 and K19 undergo caspase-mediated degradation during apoptosis. Two known K18 caspase cleavage sites are aspartates in the consensus sequences VEVDA and DALDS, located within the rod domain and tail domain, respectively. Several K14 (another type I keratin) mutations within the caspase cleavage motif have been described in patients with epidermolysis bullosa simplex. Here we use extensive mutational analysis to show that K19 and K14 are caspase substrates and that the ability to undergo caspase-mediated digestion of K18, K19, or K14 is highly dependent on the location and nature of the mutation within the caspase cleavage motif. Caspase cleavage of K14 occurs at the aspartate of VEMDA, a consensus sequence found in type I keratins K12-17 with similar but not identical sequences in K18 and K19. For K18, apoptosis-induced cleavage occurs sequentially, first at 393DALD and then at 234VEVD. Hyperphosphorylation of K18 protects from caspase-3 in vitro digestion at 234VEVD but not at 393DALD. Hence, keratins K12-17 are likely caspase substrates during apoptosis. Keratin hyperphosphorylation, which occurs early in apoptosis, protects from caspase-mediated K18 digestion in a cleavage site-specific manner. Mutations in epidermolysis bullosa simplex patients could interfere with K14 degradation during apoptosis, depending on their location.
AB - Type I keratins K18 and K19 undergo caspase-mediated degradation during apoptosis. Two known K18 caspase cleavage sites are aspartates in the consensus sequences VEVDA and DALDS, located within the rod domain and tail domain, respectively. Several K14 (another type I keratin) mutations within the caspase cleavage motif have been described in patients with epidermolysis bullosa simplex. Here we use extensive mutational analysis to show that K19 and K14 are caspase substrates and that the ability to undergo caspase-mediated digestion of K18, K19, or K14 is highly dependent on the location and nature of the mutation within the caspase cleavage motif. Caspase cleavage of K14 occurs at the aspartate of VEMDA, a consensus sequence found in type I keratins K12-17 with similar but not identical sequences in K18 and K19. For K18, apoptosis-induced cleavage occurs sequentially, first at 393DALD and then at 234VEVD. Hyperphosphorylation of K18 protects from caspase-3 in vitro digestion at 234VEVD but not at 393DALD. Hence, keratins K12-17 are likely caspase substrates during apoptosis. Keratin hyperphosphorylation, which occurs early in apoptosis, protects from caspase-mediated K18 digestion in a cleavage site-specific manner. Mutations in epidermolysis bullosa simplex patients could interfere with K14 degradation during apoptosis, depending on their location.
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U2 - 10.1074/jbc.M103315200
DO - 10.1074/jbc.M103315200
M3 - Article
C2 - 11356849
AN - SCOPUS:0035920194
VL - 276
SP - 26792
EP - 26798
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 29
ER -