Effect of nitric oxide on hydrogen peroxide-induced damage in isolated rabbit gastric glands

Hyeyoung Kim, Kyung Hwan Kim

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The present study aims at investigating the effects of nitric oxide (NO) on hydrogen peroxide (H2O2)-induced damage in isolated rabbit gastric glands. NO synthesis modulators such as L-arginine and N(G)-nitro-L-arginine methyl ester (L-NAME) and an NO donor, sodium nitroprusside, were added to isolated rabbit gastric glands exposed to H2O2, generated by glucose oxidase acting on β-D-glucose. As a result, glucose/glucose oxidase caused an increase in lipid peroxide production and decreases in reduced glutathione (GSH) content, GSH peroxidase activity, nitrite release, and mucus secretion in gastric glands. The alterations in lipid peroxide production, GSH content, and mucus secretion were prevented by pretreatment with L-arginine, a substrate for NO synthase and sodium nitroprusside, but not by L-NAME. In conclusion, NO protects gastric glands from H2O2 by inhibiting lipid peroxidation and maintaining cellular GSH content and mucus secretion.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalPharmacology
Volume57
Issue number6
DOIs
Publication statusPublished - 1998 Dec

All Science Journal Classification (ASJC) codes

  • Pharmacology

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