Effect of nucleus pulposus cells having different phenotypes on chondrogenic differentiation of adipose-derived stromal cells in a coculture system using porous membranes

Eun Hee Choi, Hansoo Park, Kwang Sook Park, Kyung Soon Park, Byung Soo Kim, In Bo Han, Dong Ah Shin, Soo Hong Lee

Research output: Contribution to journalArticle

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Abstract

In this study, adipose-derived stem cells (ASCs) were cocultured with nucleus pulposus (NP) cells using a porous membrane to investigate the effect of NP cell phenotype on ASC chondrogenic differentiation. Human NP cells were collected from 14 patients and classified into two groups (normal vs. degenerative) depending on the level of type II collagen, aggrecan (AGG), type I collagen, and bax gene expression. Human ASCs were then cocultured with each group of NP cells on porous membranes in the absence of chondrogenic supplements. After 2 weeks, real-time-polymerase chain reaction results showed that ASCs cocultured with normal NP cells had much higher type II collagen and AGG gene expression than ASCs cocultured with degenerative NP cells. The production of AGG was also observed only in the group cocultured with normal NP cells. Additionally, coculture of ASC pellets with normal NP cells promoted the production of AGG as compared to coculture of ASC monolayer with normal NP cells. These data demonstrate that a coculture system using porous membranes can induce ASC differentiation into NP cells without chondrogenic supplements. Further, the phenotype of cocultured NP cells significantly influences the extent of ASC differentiation.

Original languageEnglish
Pages (from-to)2445-2451
Number of pages7
JournalTissue Engineering - Part A
Volume17
Issue number19-20
DOIs
Publication statusPublished - 2011 Oct 1

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Stromal Cells
Coculture Techniques
Stem cells
Membranes
Phenotype
Stem Cells
Aggrecans
Collagen
Collagen Type II
Cell Differentiation
Gene expression
Nucleus Pulposus
Polymerase chain reaction
Gene Expression
Collagen Type I
Monolayers
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Choi, Eun Hee ; Park, Hansoo ; Park, Kwang Sook ; Park, Kyung Soon ; Kim, Byung Soo ; Han, In Bo ; Shin, Dong Ah ; Lee, Soo Hong. / Effect of nucleus pulposus cells having different phenotypes on chondrogenic differentiation of adipose-derived stromal cells in a coculture system using porous membranes. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 19-20. pp. 2445-2451.
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Effect of nucleus pulposus cells having different phenotypes on chondrogenic differentiation of adipose-derived stromal cells in a coculture system using porous membranes. / Choi, Eun Hee; Park, Hansoo; Park, Kwang Sook; Park, Kyung Soon; Kim, Byung Soo; Han, In Bo; Shin, Dong Ah; Lee, Soo Hong.

In: Tissue Engineering - Part A, Vol. 17, No. 19-20, 01.10.2011, p. 2445-2451.

Research output: Contribution to journalArticle

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N2 - In this study, adipose-derived stem cells (ASCs) were cocultured with nucleus pulposus (NP) cells using a porous membrane to investigate the effect of NP cell phenotype on ASC chondrogenic differentiation. Human NP cells were collected from 14 patients and classified into two groups (normal vs. degenerative) depending on the level of type II collagen, aggrecan (AGG), type I collagen, and bax gene expression. Human ASCs were then cocultured with each group of NP cells on porous membranes in the absence of chondrogenic supplements. After 2 weeks, real-time-polymerase chain reaction results showed that ASCs cocultured with normal NP cells had much higher type II collagen and AGG gene expression than ASCs cocultured with degenerative NP cells. The production of AGG was also observed only in the group cocultured with normal NP cells. Additionally, coculture of ASC pellets with normal NP cells promoted the production of AGG as compared to coculture of ASC monolayer with normal NP cells. These data demonstrate that a coculture system using porous membranes can induce ASC differentiation into NP cells without chondrogenic supplements. Further, the phenotype of cocultured NP cells significantly influences the extent of ASC differentiation.

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