Effect of organic solvent on tris(2,2'-bipyridyl)ruthenium(III) chemiluminescent reactions in flowing streams

Won Yong Lee, Timothy A. Nieman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of organic solvent on Ru(bpy)33+ chemiluminescent (CL) reactions is investigated in a flow injection system with oxalate and proline as model analytes. The organic solvents chosen for this study are acetonitrile, methanol, 2-propanol and acetone. It is found that all the four organic solvents tested can be used as organic modifiers in HPLC systems, which employ Ru(bpy)33+ chemiluminescence detection. Increasing the concentrations of acetonitrile, methanol and acetone in the phosphate carrier streams from 0 to 30% (v/v) results in increased CL intensities without significant increases in the background signals. In contrast, use of 2-propanol in the carrier streams from 0 to 30% (v/v) results in lower CL intensities, along with dramatic increase in the background signals. Stopped-flow experiments are performed to compare the quantum efficiency of Ru(bpy)33+ CL in 100% aqueous phosphate buffer and in 30% acetonitrile modified buffer. Increased quantum efficiency of Ru(bpy)33+ CL is observed in the acetonitrile modified buffer than that in the 100% aqueous phosphate buffer. Further studies by absorbance and fluorescence experiments reveal that the increased CL intensity in the acetonitrile modified buffer is attributed primarily to the increased excitation quantum efficiency of the Ru(bpy)33+ CL.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalAnalytica Chimica Acta
Volume334
Issue number1-2
DOIs
Publication statusPublished - 1996 Nov 20

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ruthenium
Organic solvents
Buffers
phosphate
acetone
Quantum efficiency
methanol
2-Propanol
Acetone
Methanol
oxalate
absorbance
Phosphates
Phosphate Transport Proteins
fluorescence
experiment
Chemiluminescence
Oxalates
Luminescence
Proline

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

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title = "Effect of organic solvent on tris(2,2'-bipyridyl)ruthenium(III) chemiluminescent reactions in flowing streams",
abstract = "The effect of organic solvent on Ru(bpy)33+ chemiluminescent (CL) reactions is investigated in a flow injection system with oxalate and proline as model analytes. The organic solvents chosen for this study are acetonitrile, methanol, 2-propanol and acetone. It is found that all the four organic solvents tested can be used as organic modifiers in HPLC systems, which employ Ru(bpy)33+ chemiluminescence detection. Increasing the concentrations of acetonitrile, methanol and acetone in the phosphate carrier streams from 0 to 30{\%} (v/v) results in increased CL intensities without significant increases in the background signals. In contrast, use of 2-propanol in the carrier streams from 0 to 30{\%} (v/v) results in lower CL intensities, along with dramatic increase in the background signals. Stopped-flow experiments are performed to compare the quantum efficiency of Ru(bpy)33+ CL in 100{\%} aqueous phosphate buffer and in 30{\%} acetonitrile modified buffer. Increased quantum efficiency of Ru(bpy)33+ CL is observed in the acetonitrile modified buffer than that in the 100{\%} aqueous phosphate buffer. Further studies by absorbance and fluorescence experiments reveal that the increased CL intensity in the acetonitrile modified buffer is attributed primarily to the increased excitation quantum efficiency of the Ru(bpy)33+ CL.",
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Effect of organic solvent on tris(2,2'-bipyridyl)ruthenium(III) chemiluminescent reactions in flowing streams. / Lee, Won Yong; Nieman, Timothy A.

In: Analytica Chimica Acta, Vol. 334, No. 1-2, 20.11.1996, p. 183-191.

Research output: Contribution to journalArticle

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AB - The effect of organic solvent on Ru(bpy)33+ chemiluminescent (CL) reactions is investigated in a flow injection system with oxalate and proline as model analytes. The organic solvents chosen for this study are acetonitrile, methanol, 2-propanol and acetone. It is found that all the four organic solvents tested can be used as organic modifiers in HPLC systems, which employ Ru(bpy)33+ chemiluminescence detection. Increasing the concentrations of acetonitrile, methanol and acetone in the phosphate carrier streams from 0 to 30% (v/v) results in increased CL intensities without significant increases in the background signals. In contrast, use of 2-propanol in the carrier streams from 0 to 30% (v/v) results in lower CL intensities, along with dramatic increase in the background signals. Stopped-flow experiments are performed to compare the quantum efficiency of Ru(bpy)33+ CL in 100% aqueous phosphate buffer and in 30% acetonitrile modified buffer. Increased quantum efficiency of Ru(bpy)33+ CL is observed in the acetonitrile modified buffer than that in the 100% aqueous phosphate buffer. Further studies by absorbance and fluorescence experiments reveal that the increased CL intensity in the acetonitrile modified buffer is attributed primarily to the increased excitation quantum efficiency of the Ru(bpy)33+ CL.

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