Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold

H. J. Seo, S. M. Yu, S. H. Lee, J. B. Choi, J. C. Park, J. K. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Potential application of the PLGA nanofiber/ film composite for vascular tissue regeneration was examined. The composite was prepared by solvent-casting and electro-spinning method. The PLGA nano-fiber was coated on the surface of the prepared PLGA film. The surface of the composite film was characterized by contact angle measurement and scanning electron microscope (SEM). The film made by only nano-fiber showed more hydrophobic than control (PLGA film made by solvent-casting); however, optimal amount of nano fibers coating could assist cell adhesion and spreading on the film. For human umbilical vein endothelial cells (HUVECs) proliferation on 6-day culture, the NF-30 (30min. nano-fiber spread specimen) composite was 1.5 times more cells proliferated than that of the NFO-1h (nano-fiber only specimen). The SEM images showed that the HUVECs on the NF-30 specimen spread widely than the other groups. The result indicates that the nano-fibers on films have an effect on cell adhesion and spreading because the adequate amount of nano-fibers on the surface of the films provide similar structure to natural ECM morphology. Conclusively, nanofiber/ film composite provided an adequate environment for cell proliferation and showed the potential application for vascular tissue regeneration.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages2147-2150
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: 2008 Dec 32008 Dec 6

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Other

Other13th International Conference on Biomedical Engineering, ICBME 2008
CountrySingapore
Period08/12/308/12/6

Fingerprint

Endothelial cells
Composite films
Grafts
Scaffolds
Fibers
Tissue regeneration
Cell adhesion
Cell proliferation
Nanofibers
Casting
Electron microscopes
Scanning
Military electronic countermeasures
Composite materials
Angle measurement
polylactic acid-polyglycolic acid copolymer
Cell culture
Contact angle
Coatings

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Seo, H. J., Yu, S. M., Lee, S. H., Choi, J. B., Park, J. C., & Kim, J. K. (2009). Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold. In 13th International Conference on Biomedical Engineering - ICBME 2008 (pp. 2147-2150). (IFMBE Proceedings; Vol. 23). https://doi.org/10.1007/978-3-540-92841-6_536
Seo, H. J. ; Yu, S. M. ; Lee, S. H. ; Choi, J. B. ; Park, J. C. ; Kim, J. K. / Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold. 13th International Conference on Biomedical Engineering - ICBME 2008. 2009. pp. 2147-2150 (IFMBE Proceedings).
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abstract = "Potential application of the PLGA nanofiber/ film composite for vascular tissue regeneration was examined. The composite was prepared by solvent-casting and electro-spinning method. The PLGA nano-fiber was coated on the surface of the prepared PLGA film. The surface of the composite film was characterized by contact angle measurement and scanning electron microscope (SEM). The film made by only nano-fiber showed more hydrophobic than control (PLGA film made by solvent-casting); however, optimal amount of nano fibers coating could assist cell adhesion and spreading on the film. For human umbilical vein endothelial cells (HUVECs) proliferation on 6-day culture, the NF-30 (30min. nano-fiber spread specimen) composite was 1.5 times more cells proliferated than that of the NFO-1h (nano-fiber only specimen). The SEM images showed that the HUVECs on the NF-30 specimen spread widely than the other groups. The result indicates that the nano-fibers on films have an effect on cell adhesion and spreading because the adequate amount of nano-fibers on the surface of the films provide similar structure to natural ECM morphology. Conclusively, nanofiber/ film composite provided an adequate environment for cell proliferation and showed the potential application for vascular tissue regeneration.",
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Seo, HJ, Yu, SM, Lee, SH, Choi, JB, Park, JC & Kim, JK 2009, Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold. in 13th International Conference on Biomedical Engineering - ICBME 2008. IFMBE Proceedings, vol. 23, pp. 2147-2150, 13th International Conference on Biomedical Engineering, ICBME 2008, Singapore, 08/12/3. https://doi.org/10.1007/978-3-540-92841-6_536

Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold. / Seo, H. J.; Yu, S. M.; Lee, S. H.; Choi, J. B.; Park, J. C.; Kim, J. K.

13th International Conference on Biomedical Engineering - ICBME 2008. 2009. p. 2147-2150 (IFMBE Proceedings; Vol. 23).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Potential application of the PLGA nanofiber/ film composite for vascular tissue regeneration was examined. The composite was prepared by solvent-casting and electro-spinning method. The PLGA nano-fiber was coated on the surface of the prepared PLGA film. The surface of the composite film was characterized by contact angle measurement and scanning electron microscope (SEM). The film made by only nano-fiber showed more hydrophobic than control (PLGA film made by solvent-casting); however, optimal amount of nano fibers coating could assist cell adhesion and spreading on the film. For human umbilical vein endothelial cells (HUVECs) proliferation on 6-day culture, the NF-30 (30min. nano-fiber spread specimen) composite was 1.5 times more cells proliferated than that of the NFO-1h (nano-fiber only specimen). The SEM images showed that the HUVECs on the NF-30 specimen spread widely than the other groups. The result indicates that the nano-fibers on films have an effect on cell adhesion and spreading because the adequate amount of nano-fibers on the surface of the films provide similar structure to natural ECM morphology. Conclusively, nanofiber/ film composite provided an adequate environment for cell proliferation and showed the potential application for vascular tissue regeneration.

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Seo HJ, Yu SM, Lee SH, Choi JB, Park JC, Kim JK. Effect of PLGA Nano-Fiber/Film Composite on HUVECs for Vascular Graft Scaffold. In 13th International Conference on Biomedical Engineering - ICBME 2008. 2009. p. 2147-2150. (IFMBE Proceedings). https://doi.org/10.1007/978-3-540-92841-6_536