Effect of polyamines on cellular differentiation of N. gruberi: Inhibition of translation of tubulin mRNA

Jin Uk Yoo, Kyung Soon Kwon, Hyun Il Cho, Daemyung Kim, In Kwon Chung, Young Min Kim, Tae Ho Lee, Joo Hun Lee

Research output: Contribution to journalArticle

Abstract

The effects of a polyamine, spermine, on the differentiation of Naegleria gruberi amebas into flagellates were tested. Addition of spermine at early stages of differentiation (until 40 min after the initiation of differentiation) completely inhibited the differentiation. To understand the inhibition mechanism, we examined the effect of spermine treatment on the transcription and translation of differentiation-specific genes during differentiation. Addition of spermine at early stages did not inhibit the accumulation of two differentiation-specific mRNAs, α-tubulin and Class I mRNA, significantly, but rather prevented the rapid degradation of the mRNAs in later stages of differentiation. Addition of spermine at the beginning of differentiation inhibited overall protein synthesis partially and gradually. However, translation of the α-tubulin mRNA was completely inhibited. These data suggest that the inhibition of differentiation of N. gruberi by spermine treatment did not result from the inhibition of transcription of differentiation-specific genes but from the specific inhibition of translation of the mRNAs during the differentiation.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalJournal of Microbiology
Volume35
Issue number4
Publication statusPublished - 1997 Dec 1

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Spermine
Polyamines
Protein Biosynthesis
Tubulin
Naegleria
Messenger RNA
Amoeba
RNA Stability
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Yoo, Jin Uk ; Kwon, Kyung Soon ; Cho, Hyun Il ; Kim, Daemyung ; Chung, In Kwon ; Kim, Young Min ; Lee, Tae Ho ; Lee, Joo Hun. / Effect of polyamines on cellular differentiation of N. gruberi : Inhibition of translation of tubulin mRNA. In: Journal of Microbiology. 1997 ; Vol. 35, No. 4. pp. 315-322.
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abstract = "The effects of a polyamine, spermine, on the differentiation of Naegleria gruberi amebas into flagellates were tested. Addition of spermine at early stages of differentiation (until 40 min after the initiation of differentiation) completely inhibited the differentiation. To understand the inhibition mechanism, we examined the effect of spermine treatment on the transcription and translation of differentiation-specific genes during differentiation. Addition of spermine at early stages did not inhibit the accumulation of two differentiation-specific mRNAs, α-tubulin and Class I mRNA, significantly, but rather prevented the rapid degradation of the mRNAs in later stages of differentiation. Addition of spermine at the beginning of differentiation inhibited overall protein synthesis partially and gradually. However, translation of the α-tubulin mRNA was completely inhibited. These data suggest that the inhibition of differentiation of N. gruberi by spermine treatment did not result from the inhibition of transcription of differentiation-specific genes but from the specific inhibition of translation of the mRNAs during the differentiation.",
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Effect of polyamines on cellular differentiation of N. gruberi : Inhibition of translation of tubulin mRNA. / Yoo, Jin Uk; Kwon, Kyung Soon; Cho, Hyun Il; Kim, Daemyung; Chung, In Kwon; Kim, Young Min; Lee, Tae Ho; Lee, Joo Hun.

In: Journal of Microbiology, Vol. 35, No. 4, 01.12.1997, p. 315-322.

Research output: Contribution to journalArticle

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