Effect of propofol post-treatment on blood-brain barrier integrity and cerebral edema after transient cerebral ischemia in rats

Jae Hoon Lee, Hui Song Cui, Seo Kyung Shin, Jeong Min Kim, So Yeon Kim, Jongeun Lee, Bon Nyeo Koo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Although propofol has been reported to offer neuroprotection against cerebral ischemia injury, its impact on cerebral edema following ischemia is not clear. The objective of this investigation is to evaluate the effects of propofol post-treatment on blood-brain barrier (BBB) integrity and cerebral edema after transient cerebral ischemia and its mechanism of action, focusing on modulation of aquaporins (AQPs), matrix metalloproteinases (MMPs), and hypoxia inducible factor (HIF)-1α. Cerebral ischemia was induced in male Sprague-Dawley rats (n = 78) by occlusion of the right middle cerebral artery for 1 h. For post-treatment with propofol, 1 mg kg-1 min-1 of propofol was administered for 1 h from the start of reperfusion. Nineteen rats undergoing sham surgery were also included in the investigation. Edema and BBB integrity were assessed by quantification of cerebral water content and extravasation of Evans blue, respectively, following 24 h of reperfusion. In addition, the expression of AQP-1, AQP-4, MMP-2, and MMP-9 was determined 24 h after reperfusion and the expression of HIF-1α was determined 8 h after reperfusion. Propofol post-treatment significantly reduced cerebral edema (P < 0.05) and BBB disruption (P < 0.05) compared with the saline-treated control. The expression of AQP-1, AQP-4, MMP-2, and MMP-9 at 24 h and of HIF-1α at 8 h following ischemia/reperfusion was significantly suppressed in the propofol post-treatment group (P < 0.05). Propofol post-treatment attenuated cerebral edema after transient cerebral ischemia, in association with reduced expression of AQP-1, AQP-4, MMP-2, and MMP-9. The decreased expression of AQPs and MMPs after propofol post-treatment might result from suppression of HIF-1α expression.

Original languageEnglish
Pages (from-to)2276-2286
Number of pages11
JournalNeurochemical Research
Volume38
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Transient Ischemic Attack
Brain Edema
Propofol
Blood-Brain Barrier
Rats
Hypoxia-Inducible Factor 1
Reperfusion
Aquaporin 1
Aquaporin 4
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Aquaporins
Brain Ischemia
Matrix Metalloproteinases
Ischemia
Evans Blue
Middle Cerebral Artery Infarction
Surgery
Water content
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Lee, Jae Hoon ; Cui, Hui Song ; Shin, Seo Kyung ; Kim, Jeong Min ; Kim, So Yeon ; Lee, Jongeun ; Koo, Bon Nyeo. / Effect of propofol post-treatment on blood-brain barrier integrity and cerebral edema after transient cerebral ischemia in rats. In: Neurochemical Research. 2013 ; Vol. 38, No. 11. pp. 2276-2286.
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Effect of propofol post-treatment on blood-brain barrier integrity and cerebral edema after transient cerebral ischemia in rats. / Lee, Jae Hoon; Cui, Hui Song; Shin, Seo Kyung; Kim, Jeong Min; Kim, So Yeon; Lee, Jongeun; Koo, Bon Nyeo.

In: Neurochemical Research, Vol. 38, No. 11, 01.11.2013, p. 2276-2286.

Research output: Contribution to journalArticle

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T1 - Effect of propofol post-treatment on blood-brain barrier integrity and cerebral edema after transient cerebral ischemia in rats

AU - Lee, Jae Hoon

AU - Cui, Hui Song

AU - Shin, Seo Kyung

AU - Kim, Jeong Min

AU - Kim, So Yeon

AU - Lee, Jongeun

AU - Koo, Bon Nyeo

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AB - Although propofol has been reported to offer neuroprotection against cerebral ischemia injury, its impact on cerebral edema following ischemia is not clear. The objective of this investigation is to evaluate the effects of propofol post-treatment on blood-brain barrier (BBB) integrity and cerebral edema after transient cerebral ischemia and its mechanism of action, focusing on modulation of aquaporins (AQPs), matrix metalloproteinases (MMPs), and hypoxia inducible factor (HIF)-1α. Cerebral ischemia was induced in male Sprague-Dawley rats (n = 78) by occlusion of the right middle cerebral artery for 1 h. For post-treatment with propofol, 1 mg kg-1 min-1 of propofol was administered for 1 h from the start of reperfusion. Nineteen rats undergoing sham surgery were also included in the investigation. Edema and BBB integrity were assessed by quantification of cerebral water content and extravasation of Evans blue, respectively, following 24 h of reperfusion. In addition, the expression of AQP-1, AQP-4, MMP-2, and MMP-9 was determined 24 h after reperfusion and the expression of HIF-1α was determined 8 h after reperfusion. Propofol post-treatment significantly reduced cerebral edema (P < 0.05) and BBB disruption (P < 0.05) compared with the saline-treated control. The expression of AQP-1, AQP-4, MMP-2, and MMP-9 at 24 h and of HIF-1α at 8 h following ischemia/reperfusion was significantly suppressed in the propofol post-treatment group (P < 0.05). Propofol post-treatment attenuated cerebral edema after transient cerebral ischemia, in association with reduced expression of AQP-1, AQP-4, MMP-2, and MMP-9. The decreased expression of AQPs and MMPs after propofol post-treatment might result from suppression of HIF-1α expression.

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