Effect of radiopaque Portland cement on mineralization in human dental pulp cells

Kyung San Min, Sang Im Lee, Yoon Lee, Eun Cheol Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

Original languageEnglish
Pages (from-to)e82-e86
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume108
Issue number4
DOIs
Publication statusPublished - 2009 Oct 1

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Dental Pulp
Alkaline Phosphatase
Dental Pulp Capping
Electrons
Control Groups
Messenger RNA
Real-Time Polymerase Chain Reaction
Coloring Agents
Staining and Labeling
Proteins
alizarin
Alizarin Red S
dentin sialophosphoprotein

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

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Effect of radiopaque Portland cement on mineralization in human dental pulp cells. / Min, Kyung San; Lee, Sang Im; Lee, Yoon; Kim, Eun Cheol.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, Vol. 108, No. 4, 01.10.2009, p. e82-e86.

Research output: Contribution to journalArticle

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AB - Objective: The aim of this study was to investigate whether radiopaque Portland cement (RPC) facilitates the mineralization process in human dental pulp cells (HDPCs) compared with pure Portland cement (PC). Study design: Under a scanning electron microscope (SEM), cellular morphology was evaluated. Alkaline phosphatase (ALP) activity was analyzed, and nodule formation was assessed by performing Alizarin Red S staining. In addition, the mRNA expressions of mineralization-related proteins were evaluated by performing a real-time polymerase chain reaction. Results: On SEM evaluation, healthy HDPCs were found adhering to the surfaces of PC and RPC. The ALP activity increased in the PC and RPC groups compared with the control group at 1 day. Alizarin Red stain increased in the PC and RPC groups compared with the control group at 2 and 3 weeks. The mRNA expression of dentin sialophosphoprotein increased at 14 days in the PC and RPC groups. Conclusions: These results show that PC and RPC have similar effects in terms of mineralization and suggest that RPC also has the potential to be used as a clinically suitable pulp-capping material.

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