Effect of recombinant cagl immunization on the gastric diseases induced by helicobacter pylori in mongolian gerbils

Eun Jung Bak, Sungil Jang, Yun Hui Choi, Jin Moon Kim, Aeryun Kim, Ji Hye Kim, Gye Hyeong Woo, Yun Jung Yoo, Sung Haeng Lee, Jeong Heon Cha

Research output: Contribution to journalArticle

Abstract

Helicobacter pylori is an important factor of chronic gastritis, digestive ulcer, and stomach cancer. CagL, a virulence factor of H. pylori, is well-known as a pilus protein which acts as adhesion to host cell and a component of Type 4 secretion system. In this study, we evaluated the protective response of recombinant CagL protein (rCagL) using Mongolian gerbil animal model for H. pylori infection. The cagL gene was cloned from 26695 H. pylori followed by over-expression and purification of the protein in E. coli. Mongolian gerbils were immunized with rCagL protein mixed with aluminum adjuvant via intramuscular injections once a week during 4 weeks. At a week after the last immunization, the Mongolian gerbils were administrated with H. pylori 7.13 strain into the stomach and sacrificed to measure antibody titer on rCagL by ELISA and bacterial colonization in the stomach, and to examine the histopathological changes and cytokine expression at 6 week after challenge. Antibody titers on recombinant protein were significantly increased from a week after the first immunization. There was no significant change of the number of bacterial colony between control group and immunized group. The relative stomach weight was significantly decreased in immunized group, but the significant change of histopathological assessment was not observed in the stomach. Cytokine expression such as IL-1β and KC also was not significantly different between control and immunized groups. These results indicate that rCagL could effectively induce the formation of the specific IgG antibodies. However, bacterial colonization and histopathological lesions could not be inhibited by the immunization in the stomach, indicating not enough protection against H. pylori infection. We consider that along with CagL other adequate antigens could be needed stimulating immune response and inducing protective effects against gastric disease, and also a better adjuvant could be considered.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalKorean Journal of Microbiology
Volume48
Issue number2
DOIs
Publication statusPublished - 2012 Dec 27

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Stomach Diseases
Gerbillinae
Recombinant Proteins
Helicobacter pylori
Immunization
Stomach
Helicobacter Infections
Antibodies
Cytokines
Complement Factor H
Control Groups
Escherichia coli Proteins
Intramuscular Injections
Pylorus
Virulence Factors
Gastritis
Cellular Structures
Aluminum
Interleukin-1
Ulcer

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Bak, Eun Jung ; Jang, Sungil ; Choi, Yun Hui ; Kim, Jin Moon ; Kim, Aeryun ; Kim, Ji Hye ; Woo, Gye Hyeong ; Yoo, Yun Jung ; Lee, Sung Haeng ; Cha, Jeong Heon. / Effect of recombinant cagl immunization on the gastric diseases induced by helicobacter pylori in mongolian gerbils. In: Korean Journal of Microbiology. 2012 ; Vol. 48, No. 2. pp. 109-115.
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Effect of recombinant cagl immunization on the gastric diseases induced by helicobacter pylori in mongolian gerbils. / Bak, Eun Jung; Jang, Sungil; Choi, Yun Hui; Kim, Jin Moon; Kim, Aeryun; Kim, Ji Hye; Woo, Gye Hyeong; Yoo, Yun Jung; Lee, Sung Haeng; Cha, Jeong Heon.

In: Korean Journal of Microbiology, Vol. 48, No. 2, 27.12.2012, p. 109-115.

Research output: Contribution to journalArticle

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T1 - Effect of recombinant cagl immunization on the gastric diseases induced by helicobacter pylori in mongolian gerbils

AU - Bak, Eun Jung

AU - Jang, Sungil

AU - Choi, Yun Hui

AU - Kim, Jin Moon

AU - Kim, Aeryun

AU - Kim, Ji Hye

AU - Woo, Gye Hyeong

AU - Yoo, Yun Jung

AU - Lee, Sung Haeng

AU - Cha, Jeong Heon

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