Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects

Seong Hee Ahn, Chang Sung Kim, Hun Joo Suk, Yong Jun Lee, Seong Ho Choi, Jung Kiu Chai, Chong Kwan Kim, Soo Boo Han, Kyoo Sung Cho

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45 Citations (Scopus)

Abstract

Background: Bone morphogenetic proteins (BMPs) are being evaluated as candidates for periodontal and bone regenerative therapy. However, the research on recombinant human bone morphogenetic protein-4 (rhBMP-4) has been insufficient to evaluate its capacity to enhance bone formation and its carrier system. The purpose of this study was to evaluate the bone regenerative effect of rhBMP-4 delivered with an absorbable collagen sponge (ACS) or β-tricalcium phosphate (β-TCP). We also compared the potential of β-TCP to that of ACS as a carrier system for rhBMP-4. Methods: Eight-mm calvarial critical-sized defects were created in 100 male Sprague-Dawley rats. The animals were divided into 5 groups of 20 animals each. The defects were treated with rhBMP-4/ACS (rhBMP-4 at 0.05 mg/ml), rhBMP-4/β-TCP (rhBMP-4 at 0.05 mg/ml), ACS alone, β-TCP alone, or left untreated for surgical control. The rats were sacrificed at 2 or 8 weeks postsurgery, and the results were evaluated radiodensitometrically, histologically, and histomorphometrically. Results: The results of radiodensitometric analysis were as follows: the rhBMP-4/ACS and the rhBMP-4/β-TCP groups were more radiopaque than other groups at both 2 and 8 weeks (P <0.01). The histologic observations were as follows: in the rhBMP-4/ACS and the rhBMP-4/β-TCP groups, new bone was evident at the defect sites at 2 weeks and 8 weeks. The results of histomorphometric analysis were as follows: the rhBMP-4/ACS and the rhBMP-4/β-TCP groups had more bone (%) than other groups at both 2 and 8 weeks (P <0.01). Conclusions: Surgical implantation of rhBMP-4/ACS may be used to support bone regeneration in the rat calvarial critical-sized defect, and rhBMP-4/β-TCP may be able to regenerate bone in the rat calvarial critical-sized defect without complication. In addition, both ACS and β-TCP may be considered as available carriers for rhBMP-4.

Original languageEnglish
Pages (from-to)787-797
Number of pages11
JournalJournal of Periodontology
Volume74
Issue number6
DOIs
Publication statusPublished - 2003 Jun 1

All Science Journal Classification (ASJC) codes

  • Periodontics

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