TY - JOUR
T1 - Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors
AU - Cha, Kyoung Je
AU - Park, Kwang Sook
AU - Kang, Sun Woong
AU - Cha, Byung Hyun
AU - Lee, Bong Kee
AU - Han, In Bo
AU - Shin, Dong Ah
AU - Kim, Dong Sung
AU - Lee, Soo Hong
PY - 2011/10/10
Y1 - 2011/10/10
N2 - We fabricated polystyrene substrates with lotus leaf surface structure (LLSS) and investigated cell behaviors, including attachment, morphology, proliferation, and differentiation of adipose-derived stem cells (ASCs) on them. Compared to the flat substrate, the LLSS substrate induced higher cell attachment rate, but did not significantly change the cell proliferation rate. In addition, ASCs on the LLSS substrate exhibited relatively narrower spreading morphology and less organized cytoskeleton, there by resulting in smaller sizes of cells than those on the flat substrate. According to histochemical staining and RT-PCR analysis, the LLSS substrate induced higher adipogenic differentiation of ASCs than the flat substrate, while chondrogenic and osteogenic differentiation were decreased.
AB - We fabricated polystyrene substrates with lotus leaf surface structure (LLSS) and investigated cell behaviors, including attachment, morphology, proliferation, and differentiation of adipose-derived stem cells (ASCs) on them. Compared to the flat substrate, the LLSS substrate induced higher cell attachment rate, but did not significantly change the cell proliferation rate. In addition, ASCs on the LLSS substrate exhibited relatively narrower spreading morphology and less organized cytoskeleton, there by resulting in smaller sizes of cells than those on the flat substrate. According to histochemical staining and RT-PCR analysis, the LLSS substrate induced higher adipogenic differentiation of ASCs than the flat substrate, while chondrogenic and osteogenic differentiation were decreased.
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U2 - 10.1002/mabi.201100134
DO - 10.1002/mabi.201100134
M3 - Article
C2 - 21793216
AN - SCOPUS:80053917010
SN - 1616-5187
VL - 11
SP - 1357
EP - 1363
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 10
ER -