Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors

Kyoung Je Cha, Kwang Sook Park, Sun Woong Kang, Byung Hyun Cha, Bong Kee Lee, In Bo Han, DongAh Shin, Dong Sung Kim, Soo Hong Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We fabricated polystyrene substrates with lotus leaf surface structure (LLSS) and investigated cell behaviors, including attachment, morphology, proliferation, and differentiation of adipose-derived stem cells (ASCs) on them. Compared to the flat substrate, the LLSS substrate induced higher cell attachment rate, but did not significantly change the cell proliferation rate. In addition, ASCs on the LLSS substrate exhibited relatively narrower spreading morphology and less organized cytoskeleton, there by resulting in smaller sizes of cells than those on the flat substrate. According to histochemical staining and RT-PCR analysis, the LLSS substrate induced higher adipogenic differentiation of ASCs than the flat substrate, while chondrogenic and osteogenic differentiation were decreased.

Original languageEnglish
Pages (from-to)1357-1363
Number of pages7
JournalMacromolecular Bioscience
Volume11
Issue number10
DOIs
Publication statusPublished - 2011 Oct 10

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Stem cells
Surface structure
Stem Cells
Substrates
Polystyrenes
Cytoskeleton
Cell Size
Cell Proliferation
Staining and Labeling
Polymerase Chain Reaction
Cell proliferation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Cha, K. J., Park, K. S., Kang, S. W., Cha, B. H., Lee, B. K., Han, I. B., ... Lee, S. H. (2011). Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors. Macromolecular Bioscience, 11(10), 1357-1363. https://doi.org/10.1002/mabi.201100134
Cha, Kyoung Je ; Park, Kwang Sook ; Kang, Sun Woong ; Cha, Byung Hyun ; Lee, Bong Kee ; Han, In Bo ; Shin, DongAh ; Kim, Dong Sung ; Lee, Soo Hong. / Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors. In: Macromolecular Bioscience. 2011 ; Vol. 11, No. 10. pp. 1357-1363.
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Effect of replicated polymeric substrate with lotus surface structure on adipose-derived stem cell behaviors. / Cha, Kyoung Je; Park, Kwang Sook; Kang, Sun Woong; Cha, Byung Hyun; Lee, Bong Kee; Han, In Bo; Shin, DongAh; Kim, Dong Sung; Lee, Soo Hong.

In: Macromolecular Bioscience, Vol. 11, No. 10, 10.10.2011, p. 1357-1363.

Research output: Contribution to journalArticle

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AU - Cha, Kyoung Je

AU - Park, Kwang Sook

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AU - Cha, Byung Hyun

AU - Lee, Bong Kee

AU - Han, In Bo

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