Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter

Sang-Hyoun Kim, Sun Kee Han, Hang Sik Shin

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

The effect of substrate concentration on hydrogen production was investigated using a continuous-flow stirred-tank reactor (CSTR). Sucrose was used as a model substrate. The CSTR was started at a sucrose concentration of 30 g COD/L and exhibited stable H2 production for 271 days at inlet sucrose concentrations of 10-60 g COD/L. Hydrogen production depended on the substrate concentration such that the highest values of 1.09 mol H 2/mol hexoseadded, 1.22 mol H2/mol hexose consumed, 7.65 L H2/L/d, and 3.80 L H2/g VSS/d were recorded at a sucrose concentration of 30 g COD/L. All bacterial species detected by polymerase chain reaction-denaturing gradient gel electrophoresis analysis were H2-producing Clostridium spp. At inlet sucrose concentrations below 20 g COD/L, the H2 yield per hexose consumed decreased along with a significant decrease in the n-butyrate/acetate ratio. At the same range of sucrose concentrations, Clostridium scatologenes (an H2-consuming acetogen) was found in the sludge. At inlet sucrose concentrations over 35 g COD/L, substrate overload occurred and caused a decrease in the carbohydrate degradation efficiency and H2 yield per hexoseadded.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalProcess Biochemistry
Volume41
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

Fermenters
Sugar (sucrose)
Hydrogen production
Ribosomal DNA
Sucrose
Hydrogen
Substrates
Clostridium
Hexoses
Denaturing Gradient Gel Electrophoresis
Butyrates
Polymerase chain reaction
Carbohydrates
Sewage
Electrophoresis
Acetates
Gels
Degradation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Engineering (miscellaneous)
  • Industrial and Manufacturing Engineering

Cite this

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abstract = "The effect of substrate concentration on hydrogen production was investigated using a continuous-flow stirred-tank reactor (CSTR). Sucrose was used as a model substrate. The CSTR was started at a sucrose concentration of 30 g COD/L and exhibited stable H2 production for 271 days at inlet sucrose concentrations of 10-60 g COD/L. Hydrogen production depended on the substrate concentration such that the highest values of 1.09 mol H 2/mol hexoseadded, 1.22 mol H2/mol hexose consumed, 7.65 L H2/L/d, and 3.80 L H2/g VSS/d were recorded at a sucrose concentration of 30 g COD/L. All bacterial species detected by polymerase chain reaction-denaturing gradient gel electrophoresis analysis were H2-producing Clostridium spp. At inlet sucrose concentrations below 20 g COD/L, the H2 yield per hexose consumed decreased along with a significant decrease in the n-butyrate/acetate ratio. At the same range of sucrose concentrations, Clostridium scatologenes (an H2-consuming acetogen) was found in the sludge. At inlet sucrose concentrations over 35 g COD/L, substrate overload occurred and caused a decrease in the carbohydrate degradation efficiency and H2 yield per hexoseadded.",
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Effect of substrate concentration on hydrogen production and 16S rDNA-based analysis of the microbial community in a continuous fermenter. / Kim, Sang-Hyoun; Han, Sun Kee; Shin, Hang Sik.

In: Process Biochemistry, Vol. 41, No. 1, 01.01.2006, p. 199-207.

Research output: Contribution to journalArticle

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