Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production

Jeong Hoon Park, Do Hyung Kim, Sang-Hyoun Kim, Jeong Jun Yoon, Hee Deung Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2–5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.

Original languageEnglish
Pages (from-to)11460-11469
Number of pages10
JournalInternational Journal of Hydrogen Energy
Volume43
Issue number25
DOIs
Publication statusPublished - 2018 Jun 21

Fingerprint

Clostridium
glucose
Glucose
bacteria
Bacteria
Substrates
galactose
lactic acid
Lactic acid
Hydrogen
hydrogen
inoculum
fermentation
Fermentation
low concentrations
trends
acids
Carbon
carbon

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Park, Jeong Hoon ; Kim, Do Hyung ; Kim, Sang-Hyoun ; Yoon, Jeong Jun ; Park, Hee Deung. / Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production. In: International Journal of Hydrogen Energy. 2018 ; Vol. 43, No. 25. pp. 11460-11469.
@article{cf43173fb8dd46dcb8e037c737e8c997,
title = "Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production",
abstract = "To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2–5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.",
author = "Park, {Jeong Hoon} and Kim, {Do Hyung} and Sang-Hyoun Kim and Yoon, {Jeong Jun} and Park, {Hee Deung}",
year = "2018",
month = "6",
day = "21",
doi = "10.1016/j.ijhydene.2017.08.150",
language = "English",
volume = "43",
pages = "11460--11469",
journal = "International Journal of Hydrogen Energy",
issn = "0360-3199",
publisher = "Elsevier Limited",
number = "25",

}

Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production. / Park, Jeong Hoon; Kim, Do Hyung; Kim, Sang-Hyoun; Yoon, Jeong Jun; Park, Hee Deung.

In: International Journal of Hydrogen Energy, Vol. 43, No. 25, 21.06.2018, p. 11460-11469.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production

AU - Park, Jeong Hoon

AU - Kim, Do Hyung

AU - Kim, Sang-Hyoun

AU - Yoon, Jeong Jun

AU - Park, Hee Deung

PY - 2018/6/21

Y1 - 2018/6/21

N2 - To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2–5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.

AB - To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2–5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.

UR - http://www.scopus.com/inward/record.url?scp=85029428772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029428772&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2017.08.150

DO - 10.1016/j.ijhydene.2017.08.150

M3 - Article

AN - SCOPUS:85029428772

VL - 43

SP - 11460

EP - 11469

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

IS - 25

ER -