Effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia

Ji Hye Kim, Dong Eun Lee, K. S.Niluka Darshani Gunawardhana, Seongho Choi, Gye Hyeong Woo, JeongHeon Cha, Eun Jung Bak, Yun Jung Yoo

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6 Citations (Scopus)

Abstract

Objective. This study examined the effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia in animal models. Materials and methods. Rats were divided into normal, periodontitis, diabetic and diabetic with periodontitis groups. After injection of streptozotocin to induce diabetes, periodontitis was induced by ligation of both lower-side first molars for 30 days. Alveolar bone loss and trabecular bone volume fraction (BVF) of the mandibular condyle and tibia were estimated via hematoxylin and eosin staining and micro-computed tomography, respectively. Osteoclastogenesis of bone marrow cells isolated from tibia and femur was assayed using tartrate-resistant acid phosphatase staining. Results. The cemento-enamel junction to the alveolar bone crest distance and ratio of periodontal ligament area in the diabetic with periodontitis group were significantly increased compared to those of the periodontitis group. Mandibular condyle BVF did not differ among groups. The BVF of tibia in the diabetic and diabetic with periodontitis groups was lower than that of the normal and periodontitis groups. Osteoclastogenesis of bone marrow cells in the diabetic groups was higher than that in the non-diabetic groups. However, the BVF of tibia and osteoclastogenesis in the diabetic with periodontitis group were not significantly different than those in the diabetic group. Conclusions. Type 1 diabetes mellitus aggravates alveolar bone loss induced by periodontitis, but periodontitis does not alter the mandibular condyle and tibia bone loss induced by diabetes. Alveolar bone, mandibular condyle and tibia may have different responses to bone loss stimuli in the diabetic environment.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalActa odontologica Scandinavica
Volume72
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Mandibular Condyle
Periodontitis
Tibia
Type 1 Diabetes Mellitus
Bone and Bones
Osteogenesis
Alveolar Bone Loss
Bone Marrow Cells
Staining and Labeling
Periodontal Ligament
Hematoxylin
Eosine Yellowish-(YS)
Dental Enamel
Streptozocin
Femur
Ligation
Animal Models

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Kim, Ji Hye ; Lee, Dong Eun ; Gunawardhana, K. S.Niluka Darshani ; Choi, Seongho ; Woo, Gye Hyeong ; Cha, JeongHeon ; Bak, Eun Jung ; Yoo, Yun Jung. / Effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia. In: Acta odontologica Scandinavica. 2014 ; Vol. 72, No. 4. pp. 265-273.
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Effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia. / Kim, Ji Hye; Lee, Dong Eun; Gunawardhana, K. S.Niluka Darshani; Choi, Seongho; Woo, Gye Hyeong; Cha, JeongHeon; Bak, Eun Jung; Yoo, Yun Jung.

In: Acta odontologica Scandinavica, Vol. 72, No. 4, 01.01.2014, p. 265-273.

Research output: Contribution to journalArticle

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AU - Kim, Ji Hye

AU - Lee, Dong Eun

AU - Gunawardhana, K. S.Niluka Darshani

AU - Choi, Seongho

AU - Woo, Gye Hyeong

AU - Cha, JeongHeon

AU - Bak, Eun Jung

AU - Yoo, Yun Jung

PY - 2014/1/1

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N2 - Objective. This study examined the effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia in animal models. Materials and methods. Rats were divided into normal, periodontitis, diabetic and diabetic with periodontitis groups. After injection of streptozotocin to induce diabetes, periodontitis was induced by ligation of both lower-side first molars for 30 days. Alveolar bone loss and trabecular bone volume fraction (BVF) of the mandibular condyle and tibia were estimated via hematoxylin and eosin staining and micro-computed tomography, respectively. Osteoclastogenesis of bone marrow cells isolated from tibia and femur was assayed using tartrate-resistant acid phosphatase staining. Results. The cemento-enamel junction to the alveolar bone crest distance and ratio of periodontal ligament area in the diabetic with periodontitis group were significantly increased compared to those of the periodontitis group. Mandibular condyle BVF did not differ among groups. The BVF of tibia in the diabetic and diabetic with periodontitis groups was lower than that of the normal and periodontitis groups. Osteoclastogenesis of bone marrow cells in the diabetic groups was higher than that in the non-diabetic groups. However, the BVF of tibia and osteoclastogenesis in the diabetic with periodontitis group were not significantly different than those in the diabetic group. Conclusions. Type 1 diabetes mellitus aggravates alveolar bone loss induced by periodontitis, but periodontitis does not alter the mandibular condyle and tibia bone loss induced by diabetes. Alveolar bone, mandibular condyle and tibia may have different responses to bone loss stimuli in the diabetic environment.

AB - Objective. This study examined the effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia in animal models. Materials and methods. Rats were divided into normal, periodontitis, diabetic and diabetic with periodontitis groups. After injection of streptozotocin to induce diabetes, periodontitis was induced by ligation of both lower-side first molars for 30 days. Alveolar bone loss and trabecular bone volume fraction (BVF) of the mandibular condyle and tibia were estimated via hematoxylin and eosin staining and micro-computed tomography, respectively. Osteoclastogenesis of bone marrow cells isolated from tibia and femur was assayed using tartrate-resistant acid phosphatase staining. Results. The cemento-enamel junction to the alveolar bone crest distance and ratio of periodontal ligament area in the diabetic with periodontitis group were significantly increased compared to those of the periodontitis group. Mandibular condyle BVF did not differ among groups. The BVF of tibia in the diabetic and diabetic with periodontitis groups was lower than that of the normal and periodontitis groups. Osteoclastogenesis of bone marrow cells in the diabetic groups was higher than that in the non-diabetic groups. However, the BVF of tibia and osteoclastogenesis in the diabetic with periodontitis group were not significantly different than those in the diabetic group. Conclusions. Type 1 diabetes mellitus aggravates alveolar bone loss induced by periodontitis, but periodontitis does not alter the mandibular condyle and tibia bone loss induced by diabetes. Alveolar bone, mandibular condyle and tibia may have different responses to bone loss stimuli in the diabetic environment.

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