Effect of the ultraviolet light treatment and storage methods on the biological activity of a titanium implant surface

Sung Hwan Choi, Won Seok Jeong, Jung Yul Cha, Jae Hoon Lee, Kee Joon Lee, Hyung Seog Yu, Eun Ha Choi, Kwang Mahn Kim, Chung Ju Hwang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution, in low vacuum, and under ambient conditions after ultraviolet light (UV) treatment is comparable to that of the surface immediately after UV treatment for 15 min and that after dielectric barrier discharge (DBD) plasma treatment for 15 min. Methods Grade IV titanium discs with machined surfaces were irradiated with UV and their surface properties were evaluated immediately and after storage for 28 days in distilled H 2 O (dH 2 O), a vacuum desiccator (31.325 kPa), and a sealed container under air. Their surface characteristics were evaluated by atomic force microscopy, X-ray diffraction, contact angle analysis, and X-ray photoelectron spectroscopy. Biological activities were determined by analyzing the albumin adsorption, MC3T3-E1 cell adhesion, and cytoskeleton development. Results Hydrophilicity of titanium surfaces stored in dH 2 O was comparable to that immediately after UV treatment and higher than that immediately after DBD plasma treatment (P < 0.001). Storage in dH 2 O and in low vacuum immediately after UV treatment prevented hydrocarbon contamination and maintained elevated amounts of titanium and oxygen. After 28 days, protein adsorption, cellular adhesion, and cytoskeletal development of MC3T3-E1 cells on the titanium surfaces stored in dH 2 O were significantly enhanced compared to those stored in low vacuum and under ambient conditions while being comparable to those immediately after UV and DBD plasma treatments. Significance UV treatment of the titanium implants followed by wet storage is useful for maintaining enhanced biological activity and overcoming biological aging during shelf storage.

Original languageEnglish
Pages (from-to)1426-1435
Number of pages10
JournalDental Materials
Volume33
Issue number12
DOIs
Publication statusPublished - 2017 Dec

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Ultraviolet Rays
Bioactivity
Titanium
Vacuum
Plasmas
Adsorption
Photoelectron Spectroscopy
Surface Properties
Atomic Force Microscopy
Cell adhesion
Hydrophilicity
Hydrocarbons
Cytoskeleton
Hydrophobic and Hydrophilic Interactions
Cell Adhesion
X-Ray Diffraction
Contact angle
Surface properties
Containers
Albumins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Choi, Sung Hwan ; Jeong, Won Seok ; Cha, Jung Yul ; Lee, Jae Hoon ; Lee, Kee Joon ; Yu, Hyung Seog ; Choi, Eun Ha ; Kim, Kwang Mahn ; Hwang, Chung Ju. / Effect of the ultraviolet light treatment and storage methods on the biological activity of a titanium implant surface. In: Dental Materials. 2017 ; Vol. 33, No. 12. pp. 1426-1435.
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abstract = "Objective We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution, in low vacuum, and under ambient conditions after ultraviolet light (UV) treatment is comparable to that of the surface immediately after UV treatment for 15 min and that after dielectric barrier discharge (DBD) plasma treatment for 15 min. Methods Grade IV titanium discs with machined surfaces were irradiated with UV and their surface properties were evaluated immediately and after storage for 28 days in distilled H 2 O (dH 2 O), a vacuum desiccator (31.325 kPa), and a sealed container under air. Their surface characteristics were evaluated by atomic force microscopy, X-ray diffraction, contact angle analysis, and X-ray photoelectron spectroscopy. Biological activities were determined by analyzing the albumin adsorption, MC3T3-E1 cell adhesion, and cytoskeleton development. Results Hydrophilicity of titanium surfaces stored in dH 2 O was comparable to that immediately after UV treatment and higher than that immediately after DBD plasma treatment (P < 0.001). Storage in dH 2 O and in low vacuum immediately after UV treatment prevented hydrocarbon contamination and maintained elevated amounts of titanium and oxygen. After 28 days, protein adsorption, cellular adhesion, and cytoskeletal development of MC3T3-E1 cells on the titanium surfaces stored in dH 2 O were significantly enhanced compared to those stored in low vacuum and under ambient conditions while being comparable to those immediately after UV and DBD plasma treatments. Significance UV treatment of the titanium implants followed by wet storage is useful for maintaining enhanced biological activity and overcoming biological aging during shelf storage.",
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Effect of the ultraviolet light treatment and storage methods on the biological activity of a titanium implant surface. / Choi, Sung Hwan; Jeong, Won Seok; Cha, Jung Yul; Lee, Jae Hoon; Lee, Kee Joon; Yu, Hyung Seog; Choi, Eun Ha; Kim, Kwang Mahn; Hwang, Chung Ju.

In: Dental Materials, Vol. 33, No. 12, 12.2017, p. 1426-1435.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of the ultraviolet light treatment and storage methods on the biological activity of a titanium implant surface

AU - Choi, Sung Hwan

AU - Jeong, Won Seok

AU - Cha, Jung Yul

AU - Lee, Jae Hoon

AU - Lee, Kee Joon

AU - Yu, Hyung Seog

AU - Choi, Eun Ha

AU - Kim, Kwang Mahn

AU - Hwang, Chung Ju

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N2 - Objective We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution, in low vacuum, and under ambient conditions after ultraviolet light (UV) treatment is comparable to that of the surface immediately after UV treatment for 15 min and that after dielectric barrier discharge (DBD) plasma treatment for 15 min. Methods Grade IV titanium discs with machined surfaces were irradiated with UV and their surface properties were evaluated immediately and after storage for 28 days in distilled H 2 O (dH 2 O), a vacuum desiccator (31.325 kPa), and a sealed container under air. Their surface characteristics were evaluated by atomic force microscopy, X-ray diffraction, contact angle analysis, and X-ray photoelectron spectroscopy. Biological activities were determined by analyzing the albumin adsorption, MC3T3-E1 cell adhesion, and cytoskeleton development. Results Hydrophilicity of titanium surfaces stored in dH 2 O was comparable to that immediately after UV treatment and higher than that immediately after DBD plasma treatment (P < 0.001). Storage in dH 2 O and in low vacuum immediately after UV treatment prevented hydrocarbon contamination and maintained elevated amounts of titanium and oxygen. After 28 days, protein adsorption, cellular adhesion, and cytoskeletal development of MC3T3-E1 cells on the titanium surfaces stored in dH 2 O were significantly enhanced compared to those stored in low vacuum and under ambient conditions while being comparable to those immediately after UV and DBD plasma treatments. Significance UV treatment of the titanium implants followed by wet storage is useful for maintaining enhanced biological activity and overcoming biological aging during shelf storage.

AB - Objective We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution, in low vacuum, and under ambient conditions after ultraviolet light (UV) treatment is comparable to that of the surface immediately after UV treatment for 15 min and that after dielectric barrier discharge (DBD) plasma treatment for 15 min. Methods Grade IV titanium discs with machined surfaces were irradiated with UV and their surface properties were evaluated immediately and after storage for 28 days in distilled H 2 O (dH 2 O), a vacuum desiccator (31.325 kPa), and a sealed container under air. Their surface characteristics were evaluated by atomic force microscopy, X-ray diffraction, contact angle analysis, and X-ray photoelectron spectroscopy. Biological activities were determined by analyzing the albumin adsorption, MC3T3-E1 cell adhesion, and cytoskeleton development. Results Hydrophilicity of titanium surfaces stored in dH 2 O was comparable to that immediately after UV treatment and higher than that immediately after DBD plasma treatment (P < 0.001). Storage in dH 2 O and in low vacuum immediately after UV treatment prevented hydrocarbon contamination and maintained elevated amounts of titanium and oxygen. After 28 days, protein adsorption, cellular adhesion, and cytoskeletal development of MC3T3-E1 cells on the titanium surfaces stored in dH 2 O were significantly enhanced compared to those stored in low vacuum and under ambient conditions while being comparable to those immediately after UV and DBD plasma treatments. Significance UV treatment of the titanium implants followed by wet storage is useful for maintaining enhanced biological activity and overcoming biological aging during shelf storage.

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