Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection

Jung Yeon Lee, Hee Jin Choi, Sangnae Cho, I. Nae Park, Yeon Mok Oh, Sang Do Lee, Woo Sung Kim, Dong Soon Kim, Won Dong Kim, Tae Sun Shim

Research output: Contribution to journalArticle

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Abstract

Background: Recently, two commercialized whole-blood assays, QuantiFERON®-TB Gold (QFT) and T SPOT-TB® (SPOT), which measure the IFN-γ released in the whole blood after being incubation with mycobacterial antigens, were approved for the diagnosis of a latent tuberculosis infection (LTBI). However, there is data on whether or not the previously used PPD skin tests (TST) have any influence on the diagnostic ability of these ex-vivo IFN-γ assays. Methods: Forty-six 15 year-old students who did not appear to be infected with Mycobacterium tuberculosis were enrolled in this study. The peripheral blood was collected and used for two IFN-γ assays. The IFN-γ assays and TST were performed at the baseline (1 st ). The TST was repeated two months later (2 nd ), and the IFN-γ assays were repeated two (2 nd ) and four months (3 rd ) later only in those subjects who had negative results at the baseline in both the IFN-γ assays and TST. An induration size > 10 mm was considered to be positive in the TST. Results: The mean TST value was 3.1 ± 5.4 mm (range: 0-20). Of the 46 subjects examined, 13 subjects (28.3%) showed positive results in the two-step TST. Nine (19.6%) were SPOT-positive and only one (2.2%) was QFT-positive. The 2 nd and 3 rd QFT were carried out in 23 and 25 all-negative subjects, respectively, and all showed negative results. The 2 nd SPOT was performed in 23 subjects and only one (4.3%) showed a weak-positive result. Conclusion: Even though there were some discrepancies in the results of the two ex-vivo IFN-γ assays, it appears that their results were not influenced by a previous TST carried out in two or four months earlier.

Original languageEnglish
Pages (from-to)406-412
Number of pages7
JournalTuberculosis and Respiratory Diseases
Volume59
Issue number4
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

Latent Tuberculosis
Tuberculin Test
Skin Tests
Gold
Interferon-gamma
Tuberculin
Exercise Test
Mycobacterium tuberculosis
Students
Antigens

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Lee, Jung Yeon ; Choi, Hee Jin ; Cho, Sangnae ; Park, I. Nae ; Oh, Yeon Mok ; Lee, Sang Do ; Kim, Woo Sung ; Kim, Dong Soon ; Kim, Won Dong ; Shim, Tae Sun. / Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection. In: Tuberculosis and Respiratory Diseases. 2005 ; Vol. 59, No. 4. pp. 406-412.
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title = "Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection",
abstract = "Background: Recently, two commercialized whole-blood assays, QuantiFERON{\circledR}-TB Gold (QFT) and T SPOT-TB{\circledR} (SPOT), which measure the IFN-γ released in the whole blood after being incubation with mycobacterial antigens, were approved for the diagnosis of a latent tuberculosis infection (LTBI). However, there is data on whether or not the previously used PPD skin tests (TST) have any influence on the diagnostic ability of these ex-vivo IFN-γ assays. Methods: Forty-six 15 year-old students who did not appear to be infected with Mycobacterium tuberculosis were enrolled in this study. The peripheral blood was collected and used for two IFN-γ assays. The IFN-γ assays and TST were performed at the baseline (1 st ). The TST was repeated two months later (2 nd ), and the IFN-γ assays were repeated two (2 nd ) and four months (3 rd ) later only in those subjects who had negative results at the baseline in both the IFN-γ assays and TST. An induration size > 10 mm was considered to be positive in the TST. Results: The mean TST value was 3.1 ± 5.4 mm (range: 0-20). Of the 46 subjects examined, 13 subjects (28.3{\%}) showed positive results in the two-step TST. Nine (19.6{\%}) were SPOT-positive and only one (2.2{\%}) was QFT-positive. The 2 nd and 3 rd QFT were carried out in 23 and 25 all-negative subjects, respectively, and all showed negative results. The 2 nd SPOT was performed in 23 subjects and only one (4.3{\%}) showed a weak-positive result. Conclusion: Even though there were some discrepancies in the results of the two ex-vivo IFN-γ assays, it appears that their results were not influenced by a previous TST carried out in two or four months earlier.",
author = "Lee, {Jung Yeon} and Choi, {Hee Jin} and Sangnae Cho and Park, {I. Nae} and Oh, {Yeon Mok} and Lee, {Sang Do} and Kim, {Woo Sung} and Kim, {Dong Soon} and Kim, {Won Dong} and Shim, {Tae Sun}",
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Lee, JY, Choi, HJ, Cho, S, Park, IN, Oh, YM, Lee, SD, Kim, WS, Kim, DS, Kim, WD & Shim, TS 2005, 'Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection', Tuberculosis and Respiratory Diseases, vol. 59, no. 4, pp. 406-412. https://doi.org/10.4046/trd.2005.59.4.406

Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection. / Lee, Jung Yeon; Choi, Hee Jin; Cho, Sangnae; Park, I. Nae; Oh, Yeon Mok; Lee, Sang Do; Kim, Woo Sung; Kim, Dong Soon; Kim, Won Dong; Shim, Tae Sun.

In: Tuberculosis and Respiratory Diseases, Vol. 59, No. 4, 01.01.2005, p. 406-412.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of tuberculin skin test on ex-vivo interferon-gamma assay for latent tuberculosis infection

AU - Lee, Jung Yeon

AU - Choi, Hee Jin

AU - Cho, Sangnae

AU - Park, I. Nae

AU - Oh, Yeon Mok

AU - Lee, Sang Do

AU - Kim, Woo Sung

AU - Kim, Dong Soon

AU - Kim, Won Dong

AU - Shim, Tae Sun

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Background: Recently, two commercialized whole-blood assays, QuantiFERON®-TB Gold (QFT) and T SPOT-TB® (SPOT), which measure the IFN-γ released in the whole blood after being incubation with mycobacterial antigens, were approved for the diagnosis of a latent tuberculosis infection (LTBI). However, there is data on whether or not the previously used PPD skin tests (TST) have any influence on the diagnostic ability of these ex-vivo IFN-γ assays. Methods: Forty-six 15 year-old students who did not appear to be infected with Mycobacterium tuberculosis were enrolled in this study. The peripheral blood was collected and used for two IFN-γ assays. The IFN-γ assays and TST were performed at the baseline (1 st ). The TST was repeated two months later (2 nd ), and the IFN-γ assays were repeated two (2 nd ) and four months (3 rd ) later only in those subjects who had negative results at the baseline in both the IFN-γ assays and TST. An induration size > 10 mm was considered to be positive in the TST. Results: The mean TST value was 3.1 ± 5.4 mm (range: 0-20). Of the 46 subjects examined, 13 subjects (28.3%) showed positive results in the two-step TST. Nine (19.6%) were SPOT-positive and only one (2.2%) was QFT-positive. The 2 nd and 3 rd QFT were carried out in 23 and 25 all-negative subjects, respectively, and all showed negative results. The 2 nd SPOT was performed in 23 subjects and only one (4.3%) showed a weak-positive result. Conclusion: Even though there were some discrepancies in the results of the two ex-vivo IFN-γ assays, it appears that their results were not influenced by a previous TST carried out in two or four months earlier.

AB - Background: Recently, two commercialized whole-blood assays, QuantiFERON®-TB Gold (QFT) and T SPOT-TB® (SPOT), which measure the IFN-γ released in the whole blood after being incubation with mycobacterial antigens, were approved for the diagnosis of a latent tuberculosis infection (LTBI). However, there is data on whether or not the previously used PPD skin tests (TST) have any influence on the diagnostic ability of these ex-vivo IFN-γ assays. Methods: Forty-six 15 year-old students who did not appear to be infected with Mycobacterium tuberculosis were enrolled in this study. The peripheral blood was collected and used for two IFN-γ assays. The IFN-γ assays and TST were performed at the baseline (1 st ). The TST was repeated two months later (2 nd ), and the IFN-γ assays were repeated two (2 nd ) and four months (3 rd ) later only in those subjects who had negative results at the baseline in both the IFN-γ assays and TST. An induration size > 10 mm was considered to be positive in the TST. Results: The mean TST value was 3.1 ± 5.4 mm (range: 0-20). Of the 46 subjects examined, 13 subjects (28.3%) showed positive results in the two-step TST. Nine (19.6%) were SPOT-positive and only one (2.2%) was QFT-positive. The 2 nd and 3 rd QFT were carried out in 23 and 25 all-negative subjects, respectively, and all showed negative results. The 2 nd SPOT was performed in 23 subjects and only one (4.3%) showed a weak-positive result. Conclusion: Even though there were some discrepancies in the results of the two ex-vivo IFN-γ assays, it appears that their results were not influenced by a previous TST carried out in two or four months earlier.

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