Abstract
Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 μg/μL cDNA and 25 μL effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non-diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules. These studies support the usefulness of VEGF-transfected islet delivery using a cationic lipid reagent to achieve euglycemia using a minimal number of islets.
| Original language | English |
|---|---|
| Pages (from-to) | 513-523 |
| Number of pages | 11 |
| Journal | Experimental and Molecular Medicine |
| Volume | 37 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2005 Dec 31 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Clinical Biochemistry
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Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice. / Chae, Hee Young; lee, byungwan; Oh, Seung Hoon; Ahn, You Ran; Chung, Jae Hoon; Min, Yong Ki; Lee, Myung Shik; Lee, Moon Kyu; Kim, Kwang Won.
In: Experimental and Molecular Medicine, Vol. 37, No. 6, 31.12.2005, p. 513-523.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice
AU - Chae, Hee Young
AU - lee, byungwan
AU - Oh, Seung Hoon
AU - Ahn, You Ran
AU - Chung, Jae Hoon
AU - Min, Yong Ki
AU - Lee, Myung Shik
AU - Lee, Moon Kyu
AU - Kim, Kwang Won
PY - 2005/12/31
Y1 - 2005/12/31
N2 - Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 μg/μL cDNA and 25 μL effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non-diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules. These studies support the usefulness of VEGF-transfected islet delivery using a cationic lipid reagent to achieve euglycemia using a minimal number of islets.
AB - Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 μg/μL cDNA and 25 μL effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non-diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules. These studies support the usefulness of VEGF-transfected islet delivery using a cationic lipid reagent to achieve euglycemia using a minimal number of islets.
UR - http://www.scopus.com/inward/record.url?scp=31344434146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31344434146&partnerID=8YFLogxK
U2 - 10.1038/emm.2005.64
DO - 10.1038/emm.2005.64
M3 - Article
C2 - 16391512
AN - SCOPUS:31344434146
VL - 37
SP - 513
EP - 523
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
SN - 1226-3613
IS - 6
ER -