Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

Young Woo Eom, Jong Eun Lee, Mal Sook Yang, In Keun Jang, Hyo Eun Kim, Doo Hoon Lee, Young Jin Kim, Won Jin Park, Jee Hyun Kong, Kwang Yong Shim, Jong In Lee, Hyun Soo Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle fibers. However, the differentiation steps that are the most critical for this process are not clear. We investigated the myogenic phases of human adipose tissue-derived stem cells (hASCs) step by step and the capability of myotube formation according to the differentiation phase by cellular fusion with mouse myoblast C2C12 cells. In hASCs treated with 5-azacytidine and fibroblast growth factor-2 (FGF-2) for 1. day, the early differentiation step to express MyoD and myogenin was induced by FGF-2 treatment for 6. days. Dystrophin and myosin heavy chain (MyHC) expression was induced by hASC conditioned medium in the late differentiation step. Myotubes were observed only in hASCs undergoing the late differentiation step by cellular fusion with C2C12 cells. In contrast, hASCs that were normal or in the early stage were not involved in myotube formation. Our results indicate that stem cells expressing dystrophin and MyHC are more suitable for myotube formation by co-culture with myoblasts than normal or early differentiated stem cells expressing MyoD and myogenin.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume408
Issue number1
DOIs
Publication statusPublished - 2011 Apr 29

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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