Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

Young Woo Eom; Jong Eun Lee; Mal Sook Yang; In Keun Jang; Hyo Eun Kim; Doo Hoon Lee; Young Jin Kim; Won Jin Park; Jee Hyun Kong; Kwang Yong Shim; Jong In Lee; Hyun Soo Kim

doi:10.1016/j.bbrc.2011.04.002

Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

Young Woo Eom, Jong Eun Lee, Mal Sook Yang, In Keun Jang, Hyo Eun Kim, Doo Hoon Lee, Young Jin Kim, Won Jin Park, Jee Hyun Kong, Kwang Yong Shim, Jong In Lee, Hyun Soo Kim

Internal Medicine

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle fibers. However, the differentiation steps that are the most critical for this process are not clear. We investigated the myogenic phases of human adipose tissue-derived stem cells (hASCs) step by step and the capability of myotube formation according to the differentiation phase by cellular fusion with mouse myoblast C2C12 cells. In hASCs treated with 5-azacytidine and fibroblast growth factor-2 (FGF-2) for 1. day, the early differentiation step to express MyoD and myogenin was induced by FGF-2 treatment for 6. days. Dystrophin and myosin heavy chain (MyHC) expression was induced by hASC conditioned medium in the late differentiation step. Myotubes were observed only in hASCs undergoing the late differentiation step by cellular fusion with C2C12 cells. In contrast, hASCs that were normal or in the early stage were not involved in myotube formation. Our results indicate that stem cells expressing dystrophin and MyHC are more suitable for myotube formation by co-culture with myoblasts than normal or early differentiated stem cells expressing MyoD and myogenin.

Original language	English
Pages (from-to)	167-173
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	408
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2011.04.002
Publication status	Published - 2011 Apr 29

Bibliographical note

Funding Information:
This work was supported by a Grant from Lifeliver Co., Ltd.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2011.04.002

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Eom, Y. W., Lee, J. E., Yang, M. S., Jang, I. K., Kim, H. E., Lee, D. H., Kim, Y. J., Park, W. J., Kong, J. H., Shim, K. Y., Lee, J. I., & Kim, H. S. (2011). Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts. Biochemical and Biophysical Research Communications, 408(1), 167-173. https://doi.org/10.1016/j.bbrc.2011.04.002

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title = "Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts",

abstract = "Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle fibers. However, the differentiation steps that are the most critical for this process are not clear. We investigated the myogenic phases of human adipose tissue-derived stem cells (hASCs) step by step and the capability of myotube formation according to the differentiation phase by cellular fusion with mouse myoblast C2C12 cells. In hASCs treated with 5-azacytidine and fibroblast growth factor-2 (FGF-2) for 1. day, the early differentiation step to express MyoD and myogenin was induced by FGF-2 treatment for 6. days. Dystrophin and myosin heavy chain (MyHC) expression was induced by hASC conditioned medium in the late differentiation step. Myotubes were observed only in hASCs undergoing the late differentiation step by cellular fusion with C2C12 cells. In contrast, hASCs that were normal or in the early stage were not involved in myotube formation. Our results indicate that stem cells expressing dystrophin and MyHC are more suitable for myotube formation by co-culture with myoblasts than normal or early differentiated stem cells expressing MyoD and myogenin.",

author = "Eom, {Young Woo} and Lee, {Jong Eun} and Yang, {Mal Sook} and Jang, {In Keun} and Kim, {Hyo Eun} and Lee, {Doo Hoon} and Kim, {Young Jin} and Park, {Won Jin} and Kong, {Jee Hyun} and Shim, {Kwang Yong} and Lee, {Jong In} and Kim, {Hyun Soo}",

note = "Funding Information: This work was supported by a Grant from Lifeliver Co., Ltd.",

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day = "29",

doi = "10.1016/j.bbrc.2011.04.002",

language = "English",

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Eom, YW, Lee, JE, Yang, MS, Jang, IK, Kim, HE, Lee, DH, Kim, YJ, Park, WJ, Kong, JH, Shim, KY, Lee, JI & Kim, HS 2011, 'Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts', Biochemical and Biophysical Research Communications, vol. 408, no. 1, pp. 167-173. https://doi.org/10.1016/j.bbrc.2011.04.002

Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts. / Eom, Young Woo; Lee, Jong Eun; Yang, Mal Sook et al.

In: Biochemical and Biophysical Research Communications, Vol. 408, No. 1, 29.04.2011, p. 167-173.

Research output: Contribution to journal › Article › peer-review

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T1 - Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

AU - Eom, Young Woo

AU - Lee, Jong Eun

AU - Yang, Mal Sook

AU - Jang, In Keun

AU - Kim, Hyo Eun

AU - Lee, Doo Hoon

AU - Kim, Young Jin

AU - Park, Won Jin

AU - Kong, Jee Hyun

AU - Shim, Kwang Yong

AU - Lee, Jong In

AU - Kim, Hyun Soo

N1 - Funding Information: This work was supported by a Grant from Lifeliver Co., Ltd.

PY - 2011/4/29

Y1 - 2011/4/29

N2 - Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle fibers. However, the differentiation steps that are the most critical for this process are not clear. We investigated the myogenic phases of human adipose tissue-derived stem cells (hASCs) step by step and the capability of myotube formation according to the differentiation phase by cellular fusion with mouse myoblast C2C12 cells. In hASCs treated with 5-azacytidine and fibroblast growth factor-2 (FGF-2) for 1. day, the early differentiation step to express MyoD and myogenin was induced by FGF-2 treatment for 6. days. Dystrophin and myosin heavy chain (MyHC) expression was induced by hASC conditioned medium in the late differentiation step. Myotubes were observed only in hASCs undergoing the late differentiation step by cellular fusion with C2C12 cells. In contrast, hASCs that were normal or in the early stage were not involved in myotube formation. Our results indicate that stem cells expressing dystrophin and MyHC are more suitable for myotube formation by co-culture with myoblasts than normal or early differentiated stem cells expressing MyoD and myogenin.

AB - Stem cell therapy for muscular dystrophies requires stem cells that are able to participate in the formation of new muscle fibers. However, the differentiation steps that are the most critical for this process are not clear. We investigated the myogenic phases of human adipose tissue-derived stem cells (hASCs) step by step and the capability of myotube formation according to the differentiation phase by cellular fusion with mouse myoblast C2C12 cells. In hASCs treated with 5-azacytidine and fibroblast growth factor-2 (FGF-2) for 1. day, the early differentiation step to express MyoD and myogenin was induced by FGF-2 treatment for 6. days. Dystrophin and myosin heavy chain (MyHC) expression was induced by hASC conditioned medium in the late differentiation step. Myotubes were observed only in hASCs undergoing the late differentiation step by cellular fusion with C2C12 cells. In contrast, hASCs that were normal or in the early stage were not involved in myotube formation. Our results indicate that stem cells expressing dystrophin and MyHC are more suitable for myotube formation by co-culture with myoblasts than normal or early differentiated stem cells expressing MyoD and myogenin.

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Effective myotube formation in human adipose tissue-derived stem cells expressing dystrophin and myosin heavy chain by cellular fusion with mouse C2C12 myoblasts

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