Effects of a nitric oxide donor and nitric oxide synthase inhibitors on acid secretion of isolated rabbit gastric glands

Hyeyoung Kim, Kyung Hwan Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The present study aims to investigate the effect of nitric oxide (NO) on histamine-stimulated acid secretion of gastric glands. An NO donor, sodium nitroprusside (SNP), and inhibitors of NO synthase such as NG-nitro-L-arginine methyl ester (L-NAME) and NG-nitro-L-arginine (L-NNA) with or without L-arginine, a substrate for NO synthase, were added to isolated rabbit gastric glands with the secretagogues. Acid secretion, intracellular concentrations of cGMP and cAMP and lactic dehydrogenase (LDH) release were determined. L-NAME and L-NNA increased stimulated acid secretion, which was reversed by L-arginine. SNP inhibited stimulated acid secretion concentration-dependently; the inhibition was accompanied by an increase in the intracellular cGMP, but neither related to cAMP nor LDH release. In conclusion, NO produced in large quantity by an NO donor inhibits histamine-stimulated acid secretion of isolated rabbit gastric glands.

Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalPharmacology
Volume53
Issue number6
DOIs
Publication statusPublished - 1996

All Science Journal Classification (ASJC) codes

  • Pharmacology

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