Abstract
The present study aims to investigate the effect of nitric oxide (NO) on histamine-stimulated acid secretion of gastric glands. An NO donor, sodium nitroprusside (SNP), and inhibitors of NO synthase such as NG-nitro-L-arginine methyl ester (L-NAME) and NG-nitro-L-arginine (L-NNA) with or without L-arginine, a substrate for NO synthase, were added to isolated rabbit gastric glands with the secretagogues. Acid secretion, intracellular concentrations of cGMP and cAMP and lactic dehydrogenase (LDH) release were determined. L-NAME and L-NNA increased stimulated acid secretion, which was reversed by L-arginine. SNP inhibited stimulated acid secretion concentration-dependently; the inhibition was accompanied by an increase in the intracellular cGMP, but neither related to cAMP nor LDH release. In conclusion, NO produced in large quantity by an NO donor inhibits histamine-stimulated acid secretion of isolated rabbit gastric glands.
| Original language | English |
|---|---|
| Pages (from-to) | 331-339 |
| Number of pages | 9 |
| Journal | Pharmacology |
| Volume | 53 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1996 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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Effects of a nitric oxide donor and nitric oxide synthase inhibitors on acid secretion of isolated rabbit gastric glands. / Kim, Hye Young; Kim, Kyung Hwan.
In: Pharmacology, Vol. 53, No. 6, 01.01.1996, p. 331-339.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effects of a nitric oxide donor and nitric oxide synthase inhibitors on acid secretion of isolated rabbit gastric glands
AU - Kim, Hye Young
AU - Kim, Kyung Hwan
PY - 1996/1/1
Y1 - 1996/1/1
N2 - The present study aims to investigate the effect of nitric oxide (NO) on histamine-stimulated acid secretion of gastric glands. An NO donor, sodium nitroprusside (SNP), and inhibitors of NO synthase such as NG-nitro-L-arginine methyl ester (L-NAME) and NG-nitro-L-arginine (L-NNA) with or without L-arginine, a substrate for NO synthase, were added to isolated rabbit gastric glands with the secretagogues. Acid secretion, intracellular concentrations of cGMP and cAMP and lactic dehydrogenase (LDH) release were determined. L-NAME and L-NNA increased stimulated acid secretion, which was reversed by L-arginine. SNP inhibited stimulated acid secretion concentration-dependently; the inhibition was accompanied by an increase in the intracellular cGMP, but neither related to cAMP nor LDH release. In conclusion, NO produced in large quantity by an NO donor inhibits histamine-stimulated acid secretion of isolated rabbit gastric glands.
AB - The present study aims to investigate the effect of nitric oxide (NO) on histamine-stimulated acid secretion of gastric glands. An NO donor, sodium nitroprusside (SNP), and inhibitors of NO synthase such as NG-nitro-L-arginine methyl ester (L-NAME) and NG-nitro-L-arginine (L-NNA) with or without L-arginine, a substrate for NO synthase, were added to isolated rabbit gastric glands with the secretagogues. Acid secretion, intracellular concentrations of cGMP and cAMP and lactic dehydrogenase (LDH) release were determined. L-NAME and L-NNA increased stimulated acid secretion, which was reversed by L-arginine. SNP inhibited stimulated acid secretion concentration-dependently; the inhibition was accompanied by an increase in the intracellular cGMP, but neither related to cAMP nor LDH release. In conclusion, NO produced in large quantity by an NO donor inhibits histamine-stimulated acid secretion of isolated rabbit gastric glands.
UR - http://www.scopus.com/inward/record.url?scp=0030446552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030446552&partnerID=8YFLogxK
U2 - 10.1159/000139448
DO - 10.1159/000139448
M3 - Article
C2 - 9032797
AN - SCOPUS:0030446552
VL - 53
SP - 331
EP - 339
JO - Pharmacology
JF - Pharmacology
SN - 0031-7012
IS - 6
ER -