Effects of a non-thermal atmospheric pressure plasma jet with different gas sources and modes of treatment on the fate of human mesenchymal stem cells

Tae Yun Kang, Jae Sung Kwon, Naresh Kumar, Eun Ha Choi, Kwang Mahn Kim

Research output: Contribution to journalArticle

Abstract

Despite numerous attempts to use human mesenchymal stem cells (hMSCs) in the field of tissue engineering, the control of their differentiation remains challenging. Here, we investigated possible applications of a non-thermal atmospheric pressure plasma jet (NTAPPJ) to control the differentiation of hMSCs. An air- or nitrogen-based NTAPPJ was applied to hMSCs in culture media, either directly or by media treatment in which the cells were plated after the medium was exposed to the NTAPPJ. The durations of exposure were 1, 2, and 4 min, and the control was not exposed to the NTAPPJ. The initial attachment of the cells was assessed by a water-soluble tetrazolium assay, and the gene expression in the cells was assessed through reverse-transcription polymerase chain reaction and immunofluorescence staining. The results showed that the gene expression in the hMSCs was generally increased by the NTAPPJ exposure, but the enhancement was dependent on the conditions of the exposure, such as the source of the gas and the treatment method used. These results were attributed to the chemicals in the extracellular environment and the reactive oxygen species generated by the plasma. Hence, it was concluded that by applying the best conditions for the NTAPPJ exposure of hMSCs, the control of hMSC differentiation was possible, and therefore, exposure to an NTAPPJ is a promising method for tissue engineering.

Original languageEnglish
Article number4819
JournalApplied Sciences (Switzerland)
Volume9
Issue number22
DOIs
Publication statusPublished - 2019 Nov 1

Fingerprint

Plasma Gases
Plasma jets
stem cells
Stem cells
plasma jets
Atmospheric pressure
atmospheric pressure
Gases
gases
tissue engineering
gene expression
Tissue engineering
Gene expression
cells
polymerase chain reaction
culture media
Polymerase chain reaction
staining
Transcription
Cell culture

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Cite this

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abstract = "Despite numerous attempts to use human mesenchymal stem cells (hMSCs) in the field of tissue engineering, the control of their differentiation remains challenging. Here, we investigated possible applications of a non-thermal atmospheric pressure plasma jet (NTAPPJ) to control the differentiation of hMSCs. An air- or nitrogen-based NTAPPJ was applied to hMSCs in culture media, either directly or by media treatment in which the cells were plated after the medium was exposed to the NTAPPJ. The durations of exposure were 1, 2, and 4 min, and the control was not exposed to the NTAPPJ. The initial attachment of the cells was assessed by a water-soluble tetrazolium assay, and the gene expression in the cells was assessed through reverse-transcription polymerase chain reaction and immunofluorescence staining. The results showed that the gene expression in the hMSCs was generally increased by the NTAPPJ exposure, but the enhancement was dependent on the conditions of the exposure, such as the source of the gas and the treatment method used. These results were attributed to the chemicals in the extracellular environment and the reactive oxygen species generated by the plasma. Hence, it was concluded that by applying the best conditions for the NTAPPJ exposure of hMSCs, the control of hMSC differentiation was possible, and therefore, exposure to an NTAPPJ is a promising method for tissue engineering.",
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Effects of a non-thermal atmospheric pressure plasma jet with different gas sources and modes of treatment on the fate of human mesenchymal stem cells. / Kang, Tae Yun; Kwon, Jae Sung; Kumar, Naresh; Choi, Eun Ha; Kim, Kwang Mahn.

In: Applied Sciences (Switzerland), Vol. 9, No. 22, 4819, 01.11.2019.

Research output: Contribution to journalArticle

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AU - Kim, Kwang Mahn

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