Effects of adenosine tetraphosphate (ATPP) on vascular tone in the isolated rat aorta

J. W. Lee, Indeok Kong, Kyusang Park, S. W. Jeong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Effects of a platelet-released, naturally occurring nucleotide, adenosine 5'-tetraphosphate (ATPP) on vascular tone were analyzed in the isolated rat aorta. Under resting tension ATPP (1 ~ 100 μM) elicited concentration-dependent contractions in endothelium-intact aortic rings in contrast to the concentration-dependent relaxation with ATP. In endothelial-denuded aortic rings, ATPP induced contraction, as ATP did, but with a greater potency. α,β-methylene ATP (APCPP 50 μM), a P(2x)-purinoceptor antagonist, significantly inhibited ATPP- as well as ATP-induced contractions in the endothelium-denuded preparations suggesting that ATPP acts via P(2x)-purinoceptors. ATPP (10 ~ 100 μM) relaxed precontracted aortic rings with an intact endothelium in a concentration-dependent manner. This effect of ATPP was 3.7 fold less potent than that of ATP. However, after P(2x)-purinoceptor blockade, the effect became identical between the two nucleotides. Reactive blue 2, a selective antagonist of P(2x)-purinoceptors, significantly attenuated the ATPP-induced relaxation with no change in the ATP-induced relaxation. These results indicated that the rat aortic endothelium contains heterogeneous populations of P 2 -purinoceptors (possibly P(2y) and nucleotide receptors). Since ATPP shows dual effects depending upon the vascular tension, it may play a significant role in the physiological regulation of vascular tone.

Original languageEnglish
Pages (from-to)487-496
Number of pages10
JournalYonsei medical journal
Volume36
Issue number6
DOIs
Publication statusPublished - 1995 Jan 1

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Adenosine
Blood Vessels
Aorta
Purinergic Receptors
Adenosine Triphosphate
Endothelium
Cibacron Blue F 3GA
Nucleotides
Purinergic Antagonists
Blood Platelets
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "Effects of a platelet-released, naturally occurring nucleotide, adenosine 5'-tetraphosphate (ATPP) on vascular tone were analyzed in the isolated rat aorta. Under resting tension ATPP (1 ~ 100 μM) elicited concentration-dependent contractions in endothelium-intact aortic rings in contrast to the concentration-dependent relaxation with ATP. In endothelial-denuded aortic rings, ATPP induced contraction, as ATP did, but with a greater potency. α,β-methylene ATP (APCPP 50 μM), a P(2x)-purinoceptor antagonist, significantly inhibited ATPP- as well as ATP-induced contractions in the endothelium-denuded preparations suggesting that ATPP acts via P(2x)-purinoceptors. ATPP (10 ~ 100 μM) relaxed precontracted aortic rings with an intact endothelium in a concentration-dependent manner. This effect of ATPP was 3.7 fold less potent than that of ATP. However, after P(2x)-purinoceptor blockade, the effect became identical between the two nucleotides. Reactive blue 2, a selective antagonist of P(2x)-purinoceptors, significantly attenuated the ATPP-induced relaxation with no change in the ATP-induced relaxation. These results indicated that the rat aortic endothelium contains heterogeneous populations of P 2 -purinoceptors (possibly P(2y) and nucleotide receptors). Since ATPP shows dual effects depending upon the vascular tension, it may play a significant role in the physiological regulation of vascular tone.",
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Effects of adenosine tetraphosphate (ATPP) on vascular tone in the isolated rat aorta. / Lee, J. W.; Kong, Indeok; Park, Kyusang; Jeong, S. W.

In: Yonsei medical journal, Vol. 36, No. 6, 01.01.1995, p. 487-496.

Research output: Contribution to journalArticle

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