Effects of aging and menopause on serum interleukin-6 levels and peripheral blood mononuclear cell cytokine production in healthy nonobese women

Oh Yoen Kim, Jey Sook Chae, Jean Kyung Paik, Hee Sun Seo, Yangsoo Jang, Jean Marc Cavaillon, Jong Ho Lee

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Inappropriate interleukin-6 production is thought to play a role in the development of several age-related conditions including atherosclerosis. This study aimed to determine whether aging affects circulating interleukin-6 (IL-6) levels. Healthy, nonobese women (n=208, 44.5±0.70 years, 22.4±0.17 kg/m 2) were categorized into four age groups (22-31, 32-41, 42-51, and 52-63 years; cross-sectional study). Cytokine levels in serum and those produced from peripheral blood mononuclear cell (PBMC) were measured. The oldest group had the highest circulating levels of IL-6 and oxidized low-density lipoprotein (ox-LDL) and higher PBMC production of IL-6, tumor necrosis factor-α (TNF-α), and interleukin-1 alpha (IL-1β). Additionally, significant interactions between age and menopause were found for serum IL-6 (P=0.024), and TNF-α (P=0.011) and IL-1β (P<0.001) produced from PBMCs. Serum IL-6 levels positively correlated with age, waist-hip ratio (WHR), systolic blood pressure, circulating levels of TNF-α, IL-1β, and ox-LDL, and urinary 8-epi-prostaglandin F 2α. Multiple stepwise regression models identified the following factors for contributing to serum IL-6 levels: serum IL-1β, menopause status, WHR, and serum TNF-α in mode I (R 2=0.302); serum IL-1β, age, serum TNF-α, and WHR (β=0.197; P=0.006) in model II (R 2=0.283). Sub-analysis was performed according to menopausal status. Serum IL-6 levels were positively associated with levels of IL-6, TNF-α, and IL-1β in PBMC supernatants (unstimulated) from postmenopausal women, whereas these were negatively associated in premenopausal women. In conclusion, circulating IL-6 levels may be interactively influenced by age and menopause. Additionally, estrogen deprivation after menopause may enhance PBMC cytokine production in postmenopausal women, resulting in increased IL-6 levels which are closely related to oxidative stress.

Original languageEnglish
Pages (from-to)415-425
Number of pages11
JournalAge
Volume34
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1

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Menopause
Interleukin-6
Blood Cells
Cytokines
Interleukin-1alpha
Serum
Tumor Necrosis Factor-alpha
Waist-Hip Ratio
Blood Pressure
Prostaglandins F
Atherosclerosis
Estrogens
Oxidative Stress
Age Groups
Cross-Sectional Studies

All Science Journal Classification (ASJC) codes

  • Ageing
  • Geriatrics and Gerontology

Cite this

Kim, Oh Yoen ; Chae, Jey Sook ; Paik, Jean Kyung ; Seo, Hee Sun ; Jang, Yangsoo ; Cavaillon, Jean Marc ; Lee, Jong Ho. / Effects of aging and menopause on serum interleukin-6 levels and peripheral blood mononuclear cell cytokine production in healthy nonobese women. In: Age. 2012 ; Vol. 34, No. 2. pp. 415-425.
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abstract = "Inappropriate interleukin-6 production is thought to play a role in the development of several age-related conditions including atherosclerosis. This study aimed to determine whether aging affects circulating interleukin-6 (IL-6) levels. Healthy, nonobese women (n=208, 44.5±0.70 years, 22.4±0.17 kg/m 2) were categorized into four age groups (22-31, 32-41, 42-51, and 52-63 years; cross-sectional study). Cytokine levels in serum and those produced from peripheral blood mononuclear cell (PBMC) were measured. The oldest group had the highest circulating levels of IL-6 and oxidized low-density lipoprotein (ox-LDL) and higher PBMC production of IL-6, tumor necrosis factor-α (TNF-α), and interleukin-1 alpha (IL-1β). Additionally, significant interactions between age and menopause were found for serum IL-6 (P=0.024), and TNF-α (P=0.011) and IL-1β (P<0.001) produced from PBMCs. Serum IL-6 levels positively correlated with age, waist-hip ratio (WHR), systolic blood pressure, circulating levels of TNF-α, IL-1β, and ox-LDL, and urinary 8-epi-prostaglandin F 2α. Multiple stepwise regression models identified the following factors for contributing to serum IL-6 levels: serum IL-1β, menopause status, WHR, and serum TNF-α in mode I (R 2=0.302); serum IL-1β, age, serum TNF-α, and WHR (β=0.197; P=0.006) in model II (R 2=0.283). Sub-analysis was performed according to menopausal status. Serum IL-6 levels were positively associated with levels of IL-6, TNF-α, and IL-1β in PBMC supernatants (unstimulated) from postmenopausal women, whereas these were negatively associated in premenopausal women. In conclusion, circulating IL-6 levels may be interactively influenced by age and menopause. Additionally, estrogen deprivation after menopause may enhance PBMC cytokine production in postmenopausal women, resulting in increased IL-6 levels which are closely related to oxidative stress.",
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Effects of aging and menopause on serum interleukin-6 levels and peripheral blood mononuclear cell cytokine production in healthy nonobese women. / Kim, Oh Yoen; Chae, Jey Sook; Paik, Jean Kyung; Seo, Hee Sun; Jang, Yangsoo; Cavaillon, Jean Marc; Lee, Jong Ho.

In: Age, Vol. 34, No. 2, 01.04.2012, p. 415-425.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of aging and menopause on serum interleukin-6 levels and peripheral blood mononuclear cell cytokine production in healthy nonobese women

AU - Kim, Oh Yoen

AU - Chae, Jey Sook

AU - Paik, Jean Kyung

AU - Seo, Hee Sun

AU - Jang, Yangsoo

AU - Cavaillon, Jean Marc

AU - Lee, Jong Ho

PY - 2012/4/1

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N2 - Inappropriate interleukin-6 production is thought to play a role in the development of several age-related conditions including atherosclerosis. This study aimed to determine whether aging affects circulating interleukin-6 (IL-6) levels. Healthy, nonobese women (n=208, 44.5±0.70 years, 22.4±0.17 kg/m 2) were categorized into four age groups (22-31, 32-41, 42-51, and 52-63 years; cross-sectional study). Cytokine levels in serum and those produced from peripheral blood mononuclear cell (PBMC) were measured. The oldest group had the highest circulating levels of IL-6 and oxidized low-density lipoprotein (ox-LDL) and higher PBMC production of IL-6, tumor necrosis factor-α (TNF-α), and interleukin-1 alpha (IL-1β). Additionally, significant interactions between age and menopause were found for serum IL-6 (P=0.024), and TNF-α (P=0.011) and IL-1β (P<0.001) produced from PBMCs. Serum IL-6 levels positively correlated with age, waist-hip ratio (WHR), systolic blood pressure, circulating levels of TNF-α, IL-1β, and ox-LDL, and urinary 8-epi-prostaglandin F 2α. Multiple stepwise regression models identified the following factors for contributing to serum IL-6 levels: serum IL-1β, menopause status, WHR, and serum TNF-α in mode I (R 2=0.302); serum IL-1β, age, serum TNF-α, and WHR (β=0.197; P=0.006) in model II (R 2=0.283). Sub-analysis was performed according to menopausal status. Serum IL-6 levels were positively associated with levels of IL-6, TNF-α, and IL-1β in PBMC supernatants (unstimulated) from postmenopausal women, whereas these were negatively associated in premenopausal women. In conclusion, circulating IL-6 levels may be interactively influenced by age and menopause. Additionally, estrogen deprivation after menopause may enhance PBMC cytokine production in postmenopausal women, resulting in increased IL-6 levels which are closely related to oxidative stress.

AB - Inappropriate interleukin-6 production is thought to play a role in the development of several age-related conditions including atherosclerosis. This study aimed to determine whether aging affects circulating interleukin-6 (IL-6) levels. Healthy, nonobese women (n=208, 44.5±0.70 years, 22.4±0.17 kg/m 2) were categorized into four age groups (22-31, 32-41, 42-51, and 52-63 years; cross-sectional study). Cytokine levels in serum and those produced from peripheral blood mononuclear cell (PBMC) were measured. The oldest group had the highest circulating levels of IL-6 and oxidized low-density lipoprotein (ox-LDL) and higher PBMC production of IL-6, tumor necrosis factor-α (TNF-α), and interleukin-1 alpha (IL-1β). Additionally, significant interactions between age and menopause were found for serum IL-6 (P=0.024), and TNF-α (P=0.011) and IL-1β (P<0.001) produced from PBMCs. Serum IL-6 levels positively correlated with age, waist-hip ratio (WHR), systolic blood pressure, circulating levels of TNF-α, IL-1β, and ox-LDL, and urinary 8-epi-prostaglandin F 2α. Multiple stepwise regression models identified the following factors for contributing to serum IL-6 levels: serum IL-1β, menopause status, WHR, and serum TNF-α in mode I (R 2=0.302); serum IL-1β, age, serum TNF-α, and WHR (β=0.197; P=0.006) in model II (R 2=0.283). Sub-analysis was performed according to menopausal status. Serum IL-6 levels were positively associated with levels of IL-6, TNF-α, and IL-1β in PBMC supernatants (unstimulated) from postmenopausal women, whereas these were negatively associated in premenopausal women. In conclusion, circulating IL-6 levels may be interactively influenced by age and menopause. Additionally, estrogen deprivation after menopause may enhance PBMC cytokine production in postmenopausal women, resulting in increased IL-6 levels which are closely related to oxidative stress.

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