Effects of alginate immobilization on dynamic membrane formation and H2 fermentation from galactose

Jong–Hun –H Park, Young–Bo –B Sim, Jun Seok Kim, Sang–Hyoun –H Kim

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


A dynamic membrane (DM) is a biofilm that forms on a support material, acting as a filter to retain high-density biomass. This study aims to explain the effects of alginate immobilization on DM formation during dark-H2 fermentation. Galactose is used as a model substrate. Heat-treated anaerobic sludge, with and without immobilization, is used for an inoculum for two identical lab-scale DM bioreactors (DMBR). The DMBRs are continuously operated for more than 40 days by changing the hydraulic retention time (HRT) from 12 to 3 h. Biomass retention and H2 production performance are significantly improved at an HRT of 3 h with immobilization. The alginate-added bioreactor shows higher extracellular polymeric substance content both in the mixed liquor and the DM. At an HRT of 3 h with immobilization, the fraction of Sporolactobacillus spp. and Lactobacillus spp. increases, possibly contributing to DM formation. However, lactic-acid concentration does not increase, implying it can be further consumed by the dominant bacteria, Clostridium butyricum.

Original languageEnglish
Pages (from-to)5874-5880
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number10
Publication statusPublished - 2020 Feb 21

Bibliographical note

Funding Information:
The authors acknowledge the National Research Foundation of Korea , grant was funded by the Korean government (MSIP, No. 2017R1A2A2A07000900 ). This research was also supported by the Yonsei University Research Fund of 2018 ( 2018-22-0047 ).

Publisher Copyright:
© 2019 Hydrogen Energy Publications LLC

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


Dive into the research topics of 'Effects of alginate immobilization on dynamic membrane formation and H2 fermentation from galactose'. Together they form a unique fingerprint.

Cite this