Effects of alginate immobilization on dynamic membrane formation and H2 fermentation from galactose

Jong Hun Park, Young Bo Sim, Jun Seok Kim, Sang-Hyoun Kim

Research output: Contribution to journalArticle

Abstract

A dynamic membrane (DM) is a biofilm that forms on a support material, acting as a filter to retain high-density biomass. This study aims to explain the effects of alginate immobilization on DM formation during dark-H2 fermentation. Galactose is used as a model substrate. Heat-treated anaerobic sludge, with and without immobilization, is used for an inoculum for two identical lab-scale DM bioreactors (DMBR). The DMBRs are continuously operated for more than 40 days by changing the hydraulic retention time (HRT) from 12 to 3 h. Biomass retention and H2 production performance are significantly improved at an HRT of 3 h with immobilization. The alginate-added bioreactor shows higher extracellular polymeric substance content both in the mixed liquor and the DM. At an HRT of 3 h with immobilization, the fraction of Sporolactobacillus spp. and Lactobacillus spp. increases, possibly contributing to DM formation. However, lactic-acid concentration does not increase, implying it can be further consumed by the dominant bacteria, Clostridium butyricum.

Original languageEnglish
JournalInternational Journal of Hydrogen Energy
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

galactose
fermentation
Alginate
immobilization
Fermentation
membranes
Membranes
hydraulics
bioreactors
Hydraulics
biomass
Bioreactors
Biomass
inoculum
Clostridium
biofilms
sludge
lactic acid
Biofilms
Lactic acid

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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abstract = "A dynamic membrane (DM) is a biofilm that forms on a support material, acting as a filter to retain high-density biomass. This study aims to explain the effects of alginate immobilization on DM formation during dark-H2 fermentation. Galactose is used as a model substrate. Heat-treated anaerobic sludge, with and without immobilization, is used for an inoculum for two identical lab-scale DM bioreactors (DMBR). The DMBRs are continuously operated for more than 40 days by changing the hydraulic retention time (HRT) from 12 to 3 h. Biomass retention and H2 production performance are significantly improved at an HRT of 3 h with immobilization. The alginate-added bioreactor shows higher extracellular polymeric substance content both in the mixed liquor and the DM. At an HRT of 3 h with immobilization, the fraction of Sporolactobacillus spp. and Lactobacillus spp. increases, possibly contributing to DM formation. However, lactic-acid concentration does not increase, implying it can be further consumed by the dominant bacteria, Clostridium butyricum.",
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Effects of alginate immobilization on dynamic membrane formation and H2 fermentation from galactose. / Park, Jong Hun; Sim, Young Bo; Kim, Jun Seok; Kim, Sang-Hyoun.

In: International Journal of Hydrogen Energy, 01.01.2019.

Research output: Contribution to journalArticle

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