Effects of calcination temperature on morphological and crystallographic properties of oyster shell as biocidal agent

Kitae Park, Kambiz Sadeghi, Sarinthip Thanakkasaranee, Ye In Park, Junsoo Park, Ki Ho Nam, Haksoo Han, Jongchul Seo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


To develop biocompatible antimicrobial agent, oyster shell wastes were thermally calcined at different temperatures ranging from 300 to 1000 °C. The chemical compositions and properties of oyster shells were characterized. As such, crystallographic analysis presented that oyster shells had a hexagonal crystalline shape, and calcination process reduced their crystalline size, volume (grain dimension), and bond length, which strongly affected antimicrobial efficacy. Results showed that the main components of uncalcined and calcined oyster shells were CaCO3 and CaO, by which CaO was found to be the main antimicrobial component. Notably, calcined oyster shells showed antimicrobial potency against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus). Furthermore, cytotoxicity analysis proved that calcined oyster shells had good cell viability and low cytotoxicity. Results highlighted that calcined oyster shells, particularly those treated at 750°C, could be a biocompatible alternative to synthetic biocidal and antimicrobial agents using in food packaging, biomedical, and cosmetic industries.

Original languageEnglish
Pages (from-to)302-311
Number of pages10
JournalInternational Journal of Applied Ceramic Technology
Issue number2
Publication statusPublished - 2021 Mar 1

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) [grant number 2020R1A2B5B01001797].

Publisher Copyright:
© 2020 The American Ceramic Society

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Condensed Matter Physics
  • Marketing
  • Materials Chemistry


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