Effects of charge density and particle size of poly(styrene/(dimethylamino)ethyl methacrylate) nanoparticle for gene delivery in 293 cells

Shi Won Pang, Hyun Young Park, Yangsoo Jang, Woo Sik Kim, Jung-Hyun Kim

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To investigate factors for enhancing transfection efficiency, PS and positively charged PS nanoparticles were used as a model gene carrier system. Polymeric nanoparticles were synthesized using a simple and reproducible method of surfactant-free emulsion polymerization and charge densities of nanoparticles controlled by a two-stage shot-growth method. The morphology, charge density, and ξ-potential values of model nanoparticles were measured by SEM, the conductive method, and Zeta Plus, respectively. Four nanoparticles with different surface charge densities were adapted to investigate the capacity of condensing the gene and the enhancement of the transfection efficiency to the target cell. The cell viabilities in the presence of nanoparticles ranged between 75-110% of the control in all experiments. The highly charged nanoparticles (0.01 μC cm -2 ) fulfilled the requirements for a suitable model gene delivery system with respect to the condensing ability of DNA, complex formation, and transfection efficiency.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume26
Issue number3
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Styrene
Methacrylates
Charge density
Particle Size
genes
Nanoparticles
delivery
polystyrene
Genes
Particle size
nanoparticles
cells
Transfection
condensing
Gene Transfer Techniques
Emulsion polymerization
wireless communication
Surface charge
poly(2-(diethylamino)ethyl methacrylate)
Emulsions

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

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abstract = "To investigate factors for enhancing transfection efficiency, PS and positively charged PS nanoparticles were used as a model gene carrier system. Polymeric nanoparticles were synthesized using a simple and reproducible method of surfactant-free emulsion polymerization and charge densities of nanoparticles controlled by a two-stage shot-growth method. The morphology, charge density, and ξ-potential values of model nanoparticles were measured by SEM, the conductive method, and Zeta Plus, respectively. Four nanoparticles with different surface charge densities were adapted to investigate the capacity of condensing the gene and the enhancement of the transfection efficiency to the target cell. The cell viabilities in the presence of nanoparticles ranged between 75-110{\%} of the control in all experiments. The highly charged nanoparticles (0.01 μC cm -2 ) fulfilled the requirements for a suitable model gene delivery system with respect to the condensing ability of DNA, complex formation, and transfection efficiency.",
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Effects of charge density and particle size of poly(styrene/(dimethylamino)ethyl methacrylate) nanoparticle for gene delivery in 293 cells. / Pang, Shi Won; Park, Hyun Young; Jang, Yangsoo; Kim, Woo Sik; Kim, Jung-Hyun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 26, No. 3, 01.01.2002, p. 213-222.

Research output: Contribution to journalArticle

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