Effects of continuous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival crevicular fluid

Keejoon Lee, Young Chel Park, Hyung Seog Yu, Seongho Choi, Yun Jung Yoo

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the effects of a light continuous force and an interrupted force with weekly reactivation on interleukin-1β (IL-1β) and prostaglandin E2 (PGE 2); possible interactions between these 2 potent mediators of the bone resorption process were assessed in vivo. Ten healthy young adults (mean age 20.6 years, 2 men, 8 women) with 4 premolars extracted were assessed. In each subject, 1 maxillary canine (E1) received continuous force with a nickel-titanium coil spring. The opposite canine (E2) received an interrupted force with a screw-attached retractor; the force was reactivated weekly by 2 turns of the screw. An antagonistic canine was used as a control. Gingival crevicular fluid was collected from the distal side of each tooth, 10 times in 3 weeks, and IL-1β and PGE2 levels were measured. For E1, the IL-1β level showed a significant elevation at 24 hours and then decreased and maintained an insignificant but high mean concentration, compared with the control site. The PGE2 level showed a significant elevation at 24 hours and then decreased. For E2, a significant elevation of IL-1β level was observed at 24 hours and a greater significant elevation at 24 hours after the first reactivation, compared with the control sites. The PGE2 level increased significantly at 24 hours and remained high for 1 week. The synergistic up-regulation of PGE2 by appliance reactivation and secreted IL-1β was not evident with either type of force after 1 week. Both experimental sites showed significant tooth movement compared with the control sites at 3 weeks; however, there was no significant difference between the 2 experimental sites. A well-controlled mechanical stress with timely reactivation can effectively upregulate IL-1β secretion, but there might be limitations in increasing the mediator levels, because of the feedback mechanisms in vivo. In addition, the analysis of crevicular fluid is a useful method for assessing cellular response to orthodontic force in vivo.

Original languageEnglish
Pages (from-to)168-177
Number of pages10
JournalAmerican Journal of Orthodontics and Dentofacial Orthopedics
Volume125
Issue number2
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Gingival Crevicular Fluid
Orthodontics
Interleukin-1
Dinoprostone
Canidae
Up-Regulation
Tooth Movement Techniques
Mechanical Stress
Bicuspid
Bone Resorption
Young Adult
Tooth
Light

All Science Journal Classification (ASJC) codes

  • Orthodontics

Cite this

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title = "Effects of continuous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival crevicular fluid",
abstract = "The purpose of this study was to evaluate the effects of a light continuous force and an interrupted force with weekly reactivation on interleukin-1β (IL-1β) and prostaglandin E2 (PGE 2); possible interactions between these 2 potent mediators of the bone resorption process were assessed in vivo. Ten healthy young adults (mean age 20.6 years, 2 men, 8 women) with 4 premolars extracted were assessed. In each subject, 1 maxillary canine (E1) received continuous force with a nickel-titanium coil spring. The opposite canine (E2) received an interrupted force with a screw-attached retractor; the force was reactivated weekly by 2 turns of the screw. An antagonistic canine was used as a control. Gingival crevicular fluid was collected from the distal side of each tooth, 10 times in 3 weeks, and IL-1β and PGE2 levels were measured. For E1, the IL-1β level showed a significant elevation at 24 hours and then decreased and maintained an insignificant but high mean concentration, compared with the control site. The PGE2 level showed a significant elevation at 24 hours and then decreased. For E2, a significant elevation of IL-1β level was observed at 24 hours and a greater significant elevation at 24 hours after the first reactivation, compared with the control sites. The PGE2 level increased significantly at 24 hours and remained high for 1 week. The synergistic up-regulation of PGE2 by appliance reactivation and secreted IL-1β was not evident with either type of force after 1 week. Both experimental sites showed significant tooth movement compared with the control sites at 3 weeks; however, there was no significant difference between the 2 experimental sites. A well-controlled mechanical stress with timely reactivation can effectively upregulate IL-1β secretion, but there might be limitations in increasing the mediator levels, because of the feedback mechanisms in vivo. In addition, the analysis of crevicular fluid is a useful method for assessing cellular response to orthodontic force in vivo.",
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Effects of continuous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival crevicular fluid. / Lee, Keejoon; Park, Young Chel; Yu, Hyung Seog; Choi, Seongho; Yoo, Yun Jung.

In: American Journal of Orthodontics and Dentofacial Orthopedics, Vol. 125, No. 2, 01.01.2004, p. 168-177.

Research output: Contribution to journalArticle

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