Abstract
The purpose of this study was to evaluate the effects of a light continuous force and an interrupted force with weekly reactivation on interleukin-1β (IL-1β) and prostaglandin E2 (PGE 2); possible interactions between these 2 potent mediators of the bone resorption process were assessed in vivo. Ten healthy young adults (mean age 20.6 years, 2 men, 8 women) with 4 premolars extracted were assessed. In each subject, 1 maxillary canine (E1) received continuous force with a nickel-titanium coil spring. The opposite canine (E2) received an interrupted force with a screw-attached retractor; the force was reactivated weekly by 2 turns of the screw. An antagonistic canine was used as a control. Gingival crevicular fluid was collected from the distal side of each tooth, 10 times in 3 weeks, and IL-1β and PGE2 levels were measured. For E1, the IL-1β level showed a significant elevation at 24 hours and then decreased and maintained an insignificant but high mean concentration, compared with the control site. The PGE2 level showed a significant elevation at 24 hours and then decreased. For E2, a significant elevation of IL-1β level was observed at 24 hours and a greater significant elevation at 24 hours after the first reactivation, compared with the control sites. The PGE2 level increased significantly at 24 hours and remained high for 1 week. The synergistic up-regulation of PGE2 by appliance reactivation and secreted IL-1β was not evident with either type of force after 1 week. Both experimental sites showed significant tooth movement compared with the control sites at 3 weeks; however, there was no significant difference between the 2 experimental sites. A well-controlled mechanical stress with timely reactivation can effectively upregulate IL-1β secretion, but there might be limitations in increasing the mediator levels, because of the feedback mechanisms in vivo. In addition, the analysis of crevicular fluid is a useful method for assessing cellular response to orthodontic force in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 168-177 |
| Number of pages | 10 |
| Journal | American Journal of Orthodontics and Dentofacial Orthopedics |
| Volume | 125 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2004 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Orthodontics
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Effects of continuous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival crevicular fluid. / Lee, Keejoon; Park, Young Chel; Yu, Hyung Seog; Choi, Seongho; Yoo, Yun Jung.
In: American Journal of Orthodontics and Dentofacial Orthopedics, Vol. 125, No. 2, 01.01.2004, p. 168-177.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effects of continuous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival crevicular fluid
AU - Lee, Keejoon
AU - Park, Young Chel
AU - Yu, Hyung Seog
AU - Choi, Seongho
AU - Yoo, Yun Jung
PY - 2004/1/1
Y1 - 2004/1/1
N2 - The purpose of this study was to evaluate the effects of a light continuous force and an interrupted force with weekly reactivation on interleukin-1β (IL-1β) and prostaglandin E2 (PGE 2); possible interactions between these 2 potent mediators of the bone resorption process were assessed in vivo. Ten healthy young adults (mean age 20.6 years, 2 men, 8 women) with 4 premolars extracted were assessed. In each subject, 1 maxillary canine (E1) received continuous force with a nickel-titanium coil spring. The opposite canine (E2) received an interrupted force with a screw-attached retractor; the force was reactivated weekly by 2 turns of the screw. An antagonistic canine was used as a control. Gingival crevicular fluid was collected from the distal side of each tooth, 10 times in 3 weeks, and IL-1β and PGE2 levels were measured. For E1, the IL-1β level showed a significant elevation at 24 hours and then decreased and maintained an insignificant but high mean concentration, compared with the control site. The PGE2 level showed a significant elevation at 24 hours and then decreased. For E2, a significant elevation of IL-1β level was observed at 24 hours and a greater significant elevation at 24 hours after the first reactivation, compared with the control sites. The PGE2 level increased significantly at 24 hours and remained high for 1 week. The synergistic up-regulation of PGE2 by appliance reactivation and secreted IL-1β was not evident with either type of force after 1 week. Both experimental sites showed significant tooth movement compared with the control sites at 3 weeks; however, there was no significant difference between the 2 experimental sites. A well-controlled mechanical stress with timely reactivation can effectively upregulate IL-1β secretion, but there might be limitations in increasing the mediator levels, because of the feedback mechanisms in vivo. In addition, the analysis of crevicular fluid is a useful method for assessing cellular response to orthodontic force in vivo.
AB - The purpose of this study was to evaluate the effects of a light continuous force and an interrupted force with weekly reactivation on interleukin-1β (IL-1β) and prostaglandin E2 (PGE 2); possible interactions between these 2 potent mediators of the bone resorption process were assessed in vivo. Ten healthy young adults (mean age 20.6 years, 2 men, 8 women) with 4 premolars extracted were assessed. In each subject, 1 maxillary canine (E1) received continuous force with a nickel-titanium coil spring. The opposite canine (E2) received an interrupted force with a screw-attached retractor; the force was reactivated weekly by 2 turns of the screw. An antagonistic canine was used as a control. Gingival crevicular fluid was collected from the distal side of each tooth, 10 times in 3 weeks, and IL-1β and PGE2 levels were measured. For E1, the IL-1β level showed a significant elevation at 24 hours and then decreased and maintained an insignificant but high mean concentration, compared with the control site. The PGE2 level showed a significant elevation at 24 hours and then decreased. For E2, a significant elevation of IL-1β level was observed at 24 hours and a greater significant elevation at 24 hours after the first reactivation, compared with the control sites. The PGE2 level increased significantly at 24 hours and remained high for 1 week. The synergistic up-regulation of PGE2 by appliance reactivation and secreted IL-1β was not evident with either type of force after 1 week. Both experimental sites showed significant tooth movement compared with the control sites at 3 weeks; however, there was no significant difference between the 2 experimental sites. A well-controlled mechanical stress with timely reactivation can effectively upregulate IL-1β secretion, but there might be limitations in increasing the mediator levels, because of the feedback mechanisms in vivo. In addition, the analysis of crevicular fluid is a useful method for assessing cellular response to orthodontic force in vivo.
UR - http://www.scopus.com/inward/record.url?scp=1042277804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1042277804&partnerID=8YFLogxK
U2 - 10.1016/j.ajodo.2003.03.006
DO - 10.1016/j.ajodo.2003.03.006
M3 - Article
C2 - 14765054
AN - SCOPUS:1042277804
VL - 125
SP - 168
EP - 177
JO - American Journal of Orthodontics and Dentofacial Orthopedics
JF - American Journal of Orthodontics and Dentofacial Orthopedics
SN - 0889-5406
IS - 2
ER -