Effects of dissolving microneedle fabrication parameters on the activity of encapsulated lysozyme

Shayan Fakhraei Lahiji, Yoojung Jang, Yonghao Ma, Manita Dangol, Huisuk Yang, Mingyu Jang, Hyungil Jung

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Dissolving microneedle (DMN) is referred to a microscale needle that encapsulates drug(s) within a biodegradable polymer matrix and delivers it into the skin in a minimally invasive manner. Although vast majority of studies have emphasized DMN as an efficient drug delivery system, the activity of DMN-encapsulated proteins or antigens can be significantly affected due to a series of thermal, physical and chemical stress factors during DMN fabrication process and storage period. The objective of this study is to evaluate the effects of DMN fabrication parameters including polymer type, polymer concentration, fabrication and storage temperature, and drying conditions on the activity of the encapsulated therapeutic proteins by employing lysozyme (LYS) as a model protein. Our results indicate that a combination of low temperature fabrication, mild drying condition, specific polymer concentration, and addition of protein stabilizer can maintain the activity of encapsulated LYS up to 99.8 ± 3.8%. Overall, findings of this study highlight the importance of optimizing DMN fabrication parameters and paves way for the commercialization of an efficient delivery system for therapeutics.

Original languageEnglish
Pages (from-to)290-296
Number of pages7
JournalEuropean Journal of Pharmaceutical Sciences
Volume117
DOIs
Publication statusPublished - 2018 May 30

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this