Effects of Elevated CO 2 on Communities of Denitrifying Bacteria and Methanogens in a Temperate Marsh Microcosm

Seung Hoon Lee, Seon Young Kim, Hojeong Kang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effects of elevated CO 2 on soil bacterial community with upland vegetation have been widely studied, but limited information is available regarding responses of denitrifier and methanogen communities to elevated CO 2 in wetland ecosystems. Using restriction fragment length polymorphism (RFLP), terminal RFLP analysis, and real-time quantitative PCR, we compared communities of denitrifiers and methanogens in a laboratory-scale wetland system planted with one of three macrophytes, Typha latifolia, Scirpus lacustris, or Juncus effusus, after 110 days of incubation. Our study showed that elevated CO 2 could affect community structures of both denitrifiers and methanogens, each of which had a unique response pattern. In particular, elevated CO 2 shifted nirS-containing community with a unique structure irrespective of vegetation type. mcrA-containing community appeared to shift to community with unique types of hydrogenotrophs under elevated CO 2 conditions. The change of dissolved organic carbon driven by elevated CO 2 appeared to be related with the shift of both denitrifiers and methanogens. Overall, this study indicates that elevated CO 2 could change the community structure of denitrifiers and methanogens temporarily. These results also suggest a presence of stable dominant populations that were not substantially affected by changes in CO 2 concentration.

Original languageEnglish
Pages (from-to)485-498
Number of pages14
JournalMicrobial Ecology
Volume64
Issue number2
DOIs
Publication statusPublished - 2012 Aug 1

Fingerprint

denitrifying bacteria
methanogens
microcosm
marshes
marsh
polymorphism
community structure
bacterium
vegetation type
dissolved organic carbon
incubation
wetland
restriction fragment length polymorphism
wetlands
Schoenoplectus lacustris
Juncus effusus
vegetation
Typha latifolia
soil bacteria
macrophytes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

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title = "Effects of Elevated CO 2 on Communities of Denitrifying Bacteria and Methanogens in a Temperate Marsh Microcosm",
abstract = "The effects of elevated CO 2 on soil bacterial community with upland vegetation have been widely studied, but limited information is available regarding responses of denitrifier and methanogen communities to elevated CO 2 in wetland ecosystems. Using restriction fragment length polymorphism (RFLP), terminal RFLP analysis, and real-time quantitative PCR, we compared communities of denitrifiers and methanogens in a laboratory-scale wetland system planted with one of three macrophytes, Typha latifolia, Scirpus lacustris, or Juncus effusus, after 110 days of incubation. Our study showed that elevated CO 2 could affect community structures of both denitrifiers and methanogens, each of which had a unique response pattern. In particular, elevated CO 2 shifted nirS-containing community with a unique structure irrespective of vegetation type. mcrA-containing community appeared to shift to community with unique types of hydrogenotrophs under elevated CO 2 conditions. The change of dissolved organic carbon driven by elevated CO 2 appeared to be related with the shift of both denitrifiers and methanogens. Overall, this study indicates that elevated CO 2 could change the community structure of denitrifiers and methanogens temporarily. These results also suggest a presence of stable dominant populations that were not substantially affected by changes in CO 2 concentration.",
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Effects of Elevated CO 2 on Communities of Denitrifying Bacteria and Methanogens in a Temperate Marsh Microcosm. / Lee, Seung Hoon; Kim, Seon Young; Kang, Hojeong.

In: Microbial Ecology, Vol. 64, No. 2, 01.08.2012, p. 485-498.

Research output: Contribution to journalArticle

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