Abstract
The effects of elevated CO 2 on soil bacterial community with upland vegetation have been widely studied, but limited information is available regarding responses of denitrifier and methanogen communities to elevated CO 2 in wetland ecosystems. Using restriction fragment length polymorphism (RFLP), terminal RFLP analysis, and real-time quantitative PCR, we compared communities of denitrifiers and methanogens in a laboratory-scale wetland system planted with one of three macrophytes, Typha latifolia, Scirpus lacustris, or Juncus effusus, after 110 days of incubation. Our study showed that elevated CO 2 could affect community structures of both denitrifiers and methanogens, each of which had a unique response pattern. In particular, elevated CO 2 shifted nirS-containing community with a unique structure irrespective of vegetation type. mcrA-containing community appeared to shift to community with unique types of hydrogenotrophs under elevated CO 2 conditions. The change of dissolved organic carbon driven by elevated CO 2 appeared to be related with the shift of both denitrifiers and methanogens. Overall, this study indicates that elevated CO 2 could change the community structure of denitrifiers and methanogens temporarily. These results also suggest a presence of stable dominant populations that were not substantially affected by changes in CO 2 concentration.
| Original language | English |
|---|---|
| Pages (from-to) | 485-498 |
| Number of pages | 14 |
| Journal | Microbial Ecology |
| Volume | 64 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics
- Ecology
- Soil Science
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Effects of Elevated CO 2 on Communities of Denitrifying Bacteria and Methanogens in a Temperate Marsh Microcosm. / Lee, Seung Hoon; Kim, Seon Young; Kang, Hojeong.
In: Microbial Ecology, Vol. 64, No. 2, 01.08.2012, p. 485-498.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effects of Elevated CO 2 on Communities of Denitrifying Bacteria and Methanogens in a Temperate Marsh Microcosm
AU - Lee, Seung Hoon
AU - Kim, Seon Young
AU - Kang, Hojeong
PY - 2012/8/1
Y1 - 2012/8/1
N2 - The effects of elevated CO 2 on soil bacterial community with upland vegetation have been widely studied, but limited information is available regarding responses of denitrifier and methanogen communities to elevated CO 2 in wetland ecosystems. Using restriction fragment length polymorphism (RFLP), terminal RFLP analysis, and real-time quantitative PCR, we compared communities of denitrifiers and methanogens in a laboratory-scale wetland system planted with one of three macrophytes, Typha latifolia, Scirpus lacustris, or Juncus effusus, after 110 days of incubation. Our study showed that elevated CO 2 could affect community structures of both denitrifiers and methanogens, each of which had a unique response pattern. In particular, elevated CO 2 shifted nirS-containing community with a unique structure irrespective of vegetation type. mcrA-containing community appeared to shift to community with unique types of hydrogenotrophs under elevated CO 2 conditions. The change of dissolved organic carbon driven by elevated CO 2 appeared to be related with the shift of both denitrifiers and methanogens. Overall, this study indicates that elevated CO 2 could change the community structure of denitrifiers and methanogens temporarily. These results also suggest a presence of stable dominant populations that were not substantially affected by changes in CO 2 concentration.
AB - The effects of elevated CO 2 on soil bacterial community with upland vegetation have been widely studied, but limited information is available regarding responses of denitrifier and methanogen communities to elevated CO 2 in wetland ecosystems. Using restriction fragment length polymorphism (RFLP), terminal RFLP analysis, and real-time quantitative PCR, we compared communities of denitrifiers and methanogens in a laboratory-scale wetland system planted with one of three macrophytes, Typha latifolia, Scirpus lacustris, or Juncus effusus, after 110 days of incubation. Our study showed that elevated CO 2 could affect community structures of both denitrifiers and methanogens, each of which had a unique response pattern. In particular, elevated CO 2 shifted nirS-containing community with a unique structure irrespective of vegetation type. mcrA-containing community appeared to shift to community with unique types of hydrogenotrophs under elevated CO 2 conditions. The change of dissolved organic carbon driven by elevated CO 2 appeared to be related with the shift of both denitrifiers and methanogens. Overall, this study indicates that elevated CO 2 could change the community structure of denitrifiers and methanogens temporarily. These results also suggest a presence of stable dominant populations that were not substantially affected by changes in CO 2 concentration.
UR - http://www.scopus.com/inward/record.url?scp=84864366932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864366932&partnerID=8YFLogxK
U2 - 10.1007/s00248-012-0036-8
DO - 10.1007/s00248-012-0036-8
M3 - Article
C2 - 22441572
AN - SCOPUS:84864366932
VL - 64
SP - 485
EP - 498
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 2
ER -