Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp

Myung Woo Byun, Jae Hun Kim, Ju Woon Lee, Jung Won Park, Chein Soo Hong, Il Jun Kang

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This study was performed to evaluate the application of food irradiation technology as a method for reducing shrimp allergy without adverse effects. Shrimp heat-stable protein (HSP) was isolated and gamma irradiated at 0, 1, 3, 5, 7, or 10 kGy in the condition of solution (1 mg/ml), and fresh shrimp was also irradiated. Conformational change of irradiated HSP was monitored by means of spectrometric measures, enzyme-linked immunosorbent assay with mouse monoclonal antibody, or human patients' sera and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ability of the immunoglobulin E of patients allergic to shrimp to bind to irradiated HSP was dose dependently reduced. The amount of intact HSP in an irradiated solution was reduced by gamma irradiation, depending on the dose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the main band disappeared and the traces induced from coagulation appeared at a higher molecular weight zone. The binding ability of immunoglobulin E to allergens in the extracts from irradiated shrimp decreased, depending on the dose. The results provide a new method so that food irradiation technology can be applied to reduce allergenicity of shrimp.

Original languageEnglish
Pages (from-to)940-944
Number of pages5
JournalJournal of Food Protection
Volume63
Issue number7
DOIs
Publication statusPublished - 2000 Jul

Fingerprint

Gamma Rays
allergens
Allergens
gamma radiation
shrimp
Food Irradiation
Hot Temperature
heat
Food Technology
Sodium Dodecyl Sulfate
Immunoglobulin E
food irradiation
immunoglobulin E
Polyacrylamide Gel Electrophoresis
Proteins
polyacrylamide gel electrophoresis
proteins
dosage
allergenicity
Hypersensitivity

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Cite this

Byun, Myung Woo ; Kim, Jae Hun ; Lee, Ju Woon ; Park, Jung Won ; Hong, Chein Soo ; Kang, Il Jun. / Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. In: Journal of Food Protection. 2000 ; Vol. 63, No. 7. pp. 940-944.
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Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. / Byun, Myung Woo; Kim, Jae Hun; Lee, Ju Woon; Park, Jung Won; Hong, Chein Soo; Kang, Il Jun.

In: Journal of Food Protection, Vol. 63, No. 7, 07.2000, p. 940-944.

Research output: Contribution to journalArticle

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