Effects of genistein on early-stage cutaneous wound healing

Eunkyo Park, Seung Min Lee, In Kyung Jung, Yunsook Lim, Jung Hyun Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Wound healing occurs in three sequential phases: hemostasis and inflammation, proliferation, and remodeling. Inflammation, the earliest phase, is considered a critical period for wound healing because immune cells remove damaged tissues, foreign debris, and remaining dead tissue. Wound healing would be delayed without inflammation, and this phase is affected by antioxidation capacity. Therefore, we hypothesized that genistein, which has an antioxidant effect, might modulate the wound healing process by altering the inflammatory response. After three days of acclimation, mice were divided into three groups: control, 0.025% genistein, and 0.1% genistein. After two weeks of an experimental diet, skin wounds were induced. Wounded skin areas were imaged, and the healing rate calculated. To measure lipid peroxidation, antioxidant enzyme expression and activity, and pro-inflammatory cytokine expression, skin and liver tissues were harvested at 12, 24, 48, and 72 h. Genistein did not affect body weight. The rate of wound closure in mice fed genistein was significantly faster than in the control group during the early stage of wound healing, especially in first three days. Cu, Zn-SOD and Mn-SOD expression in wound skin tissue in the 0.1% genistein group was lower than in the control group. However, CAT expression did not differ among groups. We also found that genistein modulated NF-κB and TNF-α expression during the early stage of wound healing. The genistein group had significantly lower hepatic lipid peroxidation and higher SOD, CAT, and GPx activities than the control group. These results suggest that genistein supplementation reduces oxidative stress by increasing antioxidant capacity and modulating proinflammatory cytokine expression during the early stage of wound healing.

Original languageEnglish
Pages (from-to)514-519
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume410
Issue number3
DOIs
Publication statusPublished - 2011 Jul 8

Fingerprint

Genistein
Wound Healing
Skin
Tissue
Control Groups
Antioxidants
Inflammation
Lipid Peroxidation
Wounds and Injuries
Cytokines
Lipids
Oxidative stress
Acclimatization
Liver
Nutrition
Hemostasis
Debris
Superoxide Dismutase
Oxidative Stress
Body Weight

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Park, Eunkyo ; Lee, Seung Min ; Jung, In Kyung ; Lim, Yunsook ; Kim, Jung Hyun. / Effects of genistein on early-stage cutaneous wound healing. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 410, No. 3. pp. 514-519.
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Effects of genistein on early-stage cutaneous wound healing. / Park, Eunkyo; Lee, Seung Min; Jung, In Kyung; Lim, Yunsook; Kim, Jung Hyun.

In: Biochemical and Biophysical Research Communications, Vol. 410, No. 3, 08.07.2011, p. 514-519.

Research output: Contribution to journalArticle

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