Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis

Si Hyun Cho, Young Sik Choi, Bo Hyon Yun, Seung Joo Chon, Yeon Soo Jung, Hye Yeon Kim, Joo Hyun Park, Seok Kyo Seo, SeHoon Kim, Byung Seok Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: Lymphangiogenesis may be involved in the pathogenesis of adenomyosis. We investigated the lymphatic vessels of patients with adenomyosis, including those treated with levonorgestrel-releasing intrauterine system (LNG-IUS). Methods: Full-thickness uterine samples were obtained from patients who received hysterectomies. Twenty-one patients with adenomyosis and 17 patients with adenomyosis who were treated with LNG-IUS were included. Eighteen patients with cervical intraepithelial neoplasia served as controls. Immunohistochemical staining was performed with antibodies against podoplanin and lymphatic vessel endothelial hyaluronan receptor 1. The lymphovascular density (LVD) was analyzed in each sample by the "hot spot" method. Results: The LVDs were significantly higher in the endometrial and myometrial tissues of patients with adenomyosis compared with those of patients treated with the LNG-IUS or controls. No significant differences were noted between the LNG-IUS-treated group and controls. Evaluation of the LVDs according to the menstrual cycle showed that the differences in the endometrial tissues of the adenomyosis group and those of the LNG-IUS-treated group or the controls were more prominent during the secretory phase. Conclusions: Treatment with the LNG-IUS resulted in reduced lymphangiogenesis and LVD in the endometrial and myometrial tissues of patients with adenomyosis. Reduced lymphangiogenesis may be one mechanism by which the LNG-IUS reduces adenomyosis-related symptoms.

Original languageEnglish
Pages (from-to)352-361
Number of pages10
JournalAmerican Journal of Clinical Pathology
Volume143
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

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Adenomyosis
Lymphangiogenesis
Levonorgestrel
Lymphatic Vessels
CD44 Antigens
Control Groups
Cervical Intraepithelial Neoplasia
Menstrual Cycle
Hysterectomy
Staining and Labeling
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Cho, S. H., Choi, Y. S., Yun, B. H., Chon, S. J., Jung, Y. S., Kim, H. Y., ... Lee, B. S. (2015). Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis. American Journal of Clinical Pathology, 143(3), 352-361. https://doi.org/10.1309/AJCPP8F4SFYFVXRN
Cho, Si Hyun ; Choi, Young Sik ; Yun, Bo Hyon ; Chon, Seung Joo ; Jung, Yeon Soo ; Kim, Hye Yeon ; Park, Joo Hyun ; Seo, Seok Kyo ; Kim, SeHoon ; Lee, Byung Seok. / Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis. In: American Journal of Clinical Pathology. 2015 ; Vol. 143, No. 3. pp. 352-361.
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abstract = "Objectives: Lymphangiogenesis may be involved in the pathogenesis of adenomyosis. We investigated the lymphatic vessels of patients with adenomyosis, including those treated with levonorgestrel-releasing intrauterine system (LNG-IUS). Methods: Full-thickness uterine samples were obtained from patients who received hysterectomies. Twenty-one patients with adenomyosis and 17 patients with adenomyosis who were treated with LNG-IUS were included. Eighteen patients with cervical intraepithelial neoplasia served as controls. Immunohistochemical staining was performed with antibodies against podoplanin and lymphatic vessel endothelial hyaluronan receptor 1. The lymphovascular density (LVD) was analyzed in each sample by the {"}hot spot{"} method. Results: The LVDs were significantly higher in the endometrial and myometrial tissues of patients with adenomyosis compared with those of patients treated with the LNG-IUS or controls. No significant differences were noted between the LNG-IUS-treated group and controls. Evaluation of the LVDs according to the menstrual cycle showed that the differences in the endometrial tissues of the adenomyosis group and those of the LNG-IUS-treated group or the controls were more prominent during the secretory phase. Conclusions: Treatment with the LNG-IUS resulted in reduced lymphangiogenesis and LVD in the endometrial and myometrial tissues of patients with adenomyosis. Reduced lymphangiogenesis may be one mechanism by which the LNG-IUS reduces adenomyosis-related symptoms.",
author = "Cho, {Si Hyun} and Choi, {Young Sik} and Yun, {Bo Hyon} and Chon, {Seung Joo} and Jung, {Yeon Soo} and Kim, {Hye Yeon} and Park, {Joo Hyun} and Seo, {Seok Kyo} and SeHoon Kim and Lee, {Byung Seok}",
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Cho, SH, Choi, YS, Yun, BH, Chon, SJ, Jung, YS, Kim, HY, Park, JH, Seo, SK, Kim, S & Lee, BS 2015, 'Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis', American Journal of Clinical Pathology, vol. 143, no. 3, pp. 352-361. https://doi.org/10.1309/AJCPP8F4SFYFVXRN

Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis. / Cho, Si Hyun; Choi, Young Sik; Yun, Bo Hyon; Chon, Seung Joo; Jung, Yeon Soo; Kim, Hye Yeon; Park, Joo Hyun; Seo, Seok Kyo; Kim, SeHoon; Lee, Byung Seok.

In: American Journal of Clinical Pathology, Vol. 143, No. 3, 01.01.2015, p. 352-361.

Research output: Contribution to journalArticle

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T1 - Effects of levonorgestrel-releasing intrauterine system on lymphangiogenesis of adenomyosis

AU - Cho, Si Hyun

AU - Choi, Young Sik

AU - Yun, Bo Hyon

AU - Chon, Seung Joo

AU - Jung, Yeon Soo

AU - Kim, Hye Yeon

AU - Park, Joo Hyun

AU - Seo, Seok Kyo

AU - Kim, SeHoon

AU - Lee, Byung Seok

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Objectives: Lymphangiogenesis may be involved in the pathogenesis of adenomyosis. We investigated the lymphatic vessels of patients with adenomyosis, including those treated with levonorgestrel-releasing intrauterine system (LNG-IUS). Methods: Full-thickness uterine samples were obtained from patients who received hysterectomies. Twenty-one patients with adenomyosis and 17 patients with adenomyosis who were treated with LNG-IUS were included. Eighteen patients with cervical intraepithelial neoplasia served as controls. Immunohistochemical staining was performed with antibodies against podoplanin and lymphatic vessel endothelial hyaluronan receptor 1. The lymphovascular density (LVD) was analyzed in each sample by the "hot spot" method. Results: The LVDs were significantly higher in the endometrial and myometrial tissues of patients with adenomyosis compared with those of patients treated with the LNG-IUS or controls. No significant differences were noted between the LNG-IUS-treated group and controls. Evaluation of the LVDs according to the menstrual cycle showed that the differences in the endometrial tissues of the adenomyosis group and those of the LNG-IUS-treated group or the controls were more prominent during the secretory phase. Conclusions: Treatment with the LNG-IUS resulted in reduced lymphangiogenesis and LVD in the endometrial and myometrial tissues of patients with adenomyosis. Reduced lymphangiogenesis may be one mechanism by which the LNG-IUS reduces adenomyosis-related symptoms.

AB - Objectives: Lymphangiogenesis may be involved in the pathogenesis of adenomyosis. We investigated the lymphatic vessels of patients with adenomyosis, including those treated with levonorgestrel-releasing intrauterine system (LNG-IUS). Methods: Full-thickness uterine samples were obtained from patients who received hysterectomies. Twenty-one patients with adenomyosis and 17 patients with adenomyosis who were treated with LNG-IUS were included. Eighteen patients with cervical intraepithelial neoplasia served as controls. Immunohistochemical staining was performed with antibodies against podoplanin and lymphatic vessel endothelial hyaluronan receptor 1. The lymphovascular density (LVD) was analyzed in each sample by the "hot spot" method. Results: The LVDs were significantly higher in the endometrial and myometrial tissues of patients with adenomyosis compared with those of patients treated with the LNG-IUS or controls. No significant differences were noted between the LNG-IUS-treated group and controls. Evaluation of the LVDs according to the menstrual cycle showed that the differences in the endometrial tissues of the adenomyosis group and those of the LNG-IUS-treated group or the controls were more prominent during the secretory phase. Conclusions: Treatment with the LNG-IUS resulted in reduced lymphangiogenesis and LVD in the endometrial and myometrial tissues of patients with adenomyosis. Reduced lymphangiogenesis may be one mechanism by which the LNG-IUS reduces adenomyosis-related symptoms.

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