Abstract
Ultraviolet B (UVB) radiation induces photoaging by upregulating the expression of matrix metalloproteinase (MMP) and decreasing collagen synthesis in human skin cells. This study evaluated the effects of licarin E isolated from mace, the aril of Myristica fragrans Houtt., on MMP-1 and type-1 procollagen levels in UVB-irradiated human skin fibroblasts. Powdered mace extracted with 95% ethanol was used and licarin E isolated by preparative high-performance liquid chromatography. In addition, western blot analysis, reverse transcription PCR and electrophoretic mobility shift assay were used to evaluate the effects of licarin E and its molecular mechanism. It was found that licarin E attenuated UVB-induced MMP-1 expression by inactivating mitogen-activated protein kinases (MAPKs), thereby inhibiting activator protein 1. Licarin E also increased type-1 procollagen expression by stimulating transforming growth factor β (TGFβ)/Smad signaling. The findings show that licarin E positively regulates the expression of MMP-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts through MAPK and TGFβ signaling, suggesting its potential as a potent antiphotoaging agent.
| Original language | English |
|---|---|
| Pages (from-to) | 1891-1894 |
| Number of pages | 4 |
| Journal | Phytotherapy Research |
| Volume | 25 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2011 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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Effects of licarin e on expression of matrix metalloproteinase-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts. / Kwon, Yi Young; Kim, Daeyoung; Kim, Jaekyung; Hwang, Jae-Kwan.
In: Phytotherapy Research, Vol. 25, No. 12, 01.12.2011, p. 1891-1894.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effects of licarin e on expression of matrix metalloproteinase-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts
AU - Kwon, Yi Young
AU - Kim, Daeyoung
AU - Kim, Jaekyung
AU - Hwang, Jae-Kwan
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Ultraviolet B (UVB) radiation induces photoaging by upregulating the expression of matrix metalloproteinase (MMP) and decreasing collagen synthesis in human skin cells. This study evaluated the effects of licarin E isolated from mace, the aril of Myristica fragrans Houtt., on MMP-1 and type-1 procollagen levels in UVB-irradiated human skin fibroblasts. Powdered mace extracted with 95% ethanol was used and licarin E isolated by preparative high-performance liquid chromatography. In addition, western blot analysis, reverse transcription PCR and electrophoretic mobility shift assay were used to evaluate the effects of licarin E and its molecular mechanism. It was found that licarin E attenuated UVB-induced MMP-1 expression by inactivating mitogen-activated protein kinases (MAPKs), thereby inhibiting activator protein 1. Licarin E also increased type-1 procollagen expression by stimulating transforming growth factor β (TGFβ)/Smad signaling. The findings show that licarin E positively regulates the expression of MMP-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts through MAPK and TGFβ signaling, suggesting its potential as a potent antiphotoaging agent.
AB - Ultraviolet B (UVB) radiation induces photoaging by upregulating the expression of matrix metalloproteinase (MMP) and decreasing collagen synthesis in human skin cells. This study evaluated the effects of licarin E isolated from mace, the aril of Myristica fragrans Houtt., on MMP-1 and type-1 procollagen levels in UVB-irradiated human skin fibroblasts. Powdered mace extracted with 95% ethanol was used and licarin E isolated by preparative high-performance liquid chromatography. In addition, western blot analysis, reverse transcription PCR and electrophoretic mobility shift assay were used to evaluate the effects of licarin E and its molecular mechanism. It was found that licarin E attenuated UVB-induced MMP-1 expression by inactivating mitogen-activated protein kinases (MAPKs), thereby inhibiting activator protein 1. Licarin E also increased type-1 procollagen expression by stimulating transforming growth factor β (TGFβ)/Smad signaling. The findings show that licarin E positively regulates the expression of MMP-1 and type-1 procollagen in UVB-irradiated human skin fibroblasts through MAPK and TGFβ signaling, suggesting its potential as a potent antiphotoaging agent.
UR - http://www.scopus.com/inward/record.url?scp=82155171465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82155171465&partnerID=8YFLogxK
U2 - 10.1002/ptr.3521
DO - 10.1002/ptr.3521
M3 - Article
VL - 25
SP - 1891
EP - 1894
JO - Phytotherapy Research
JF - Phytotherapy Research
SN - 0951-418X
IS - 12
ER -