Effects of low-intensity ultrasound on oxidative damage in retinal pigment epithelial cells in vitro

Na Kyeong Kim, Chan Yun Kim, Min Joo Choi, So Ra Park, Byung Hyune Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Oxidative stress in retinal pigment epithelium (RPE) is one of the key causative factors of RPE injury in age-related macular degeneration (AMD). Low-intensity ultrasound (LIUS) less than 1 W/cm2 in intensity has been found to have cytoprotective and anti-inflammatory effects in many cell types and diseases. In this study, we investigated for the first time the feasibility of using LIUS to protect RPE cells from oxidative damage. ARPE-19 cells were treated with H2O2 (an exogenous source of reactive oxygen species) or L-buthionine-(S,R)-sulfoximine (BSO), a glutathione synthase inhibitor, and exposed immediately to LIUS at intensities of 50, 100 and 200 mW/cm2 and a frequency of 1 MHz for 20 min. Both H2O2 and BSO increased the percentage of cells positive for mitochondrial reactive oxygen species at 1 h, but not at 24 h. Co-treatment with LIUS clearly repressed these cells similarly at all intensities by approximately 34%-43% for H2O2 and 24%-25% for BSO (p < 0.05). The percentage of cells with mitochondrial membrane depolarization also increased with H2O2 and BSO treatment, particularly at 1 h, and decreased by approximately 60% with LIUS at 100 mW/cm2 (p < 0.05). The amount of intracellular calcium ion ([Ca2+]i) was elevated only by BSO at 24 h and was also significantly diminished, by approximately 45%, by LIUS at 100 mW/cm2 (p < 0.05). Both H2O2 and BSO significantly hampered cell viability at 24 h, but LIUS at 100 mW/cm2 restored only BSO-induced cell viability by approximately 2.7-fold (p < 0.05). This study illustrated that LIUS has a protective effect on RPE cells against oxidative damage caused by BSO, an endogenous mitochondrial reactive oxygen species generator. We speculate that LIUS has the potential to treat oxidative damage and related pathologic changes in RPE.

Original languageEnglish
Pages (from-to)1363-1371
Number of pages9
JournalUltrasound in Medicine and Biology
Volume41
Issue number5
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Retinal Pigments
pigments
Retinal Pigment Epithelium
Epithelial Cells
damage
epithelium
Reactive Oxygen Species
cells
Cell Survival
Glutathione Synthase
viability
Macular Degeneration
Mitochondrial Membranes
In Vitro Techniques
oxygen
Oxidative Stress
Anti-Inflammatory Agents
Ions
glutathione
degeneration

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

Cite this

Kim, Na Kyeong ; Kim, Chan Yun ; Choi, Min Joo ; Park, So Ra ; Choi, Byung Hyune. / Effects of low-intensity ultrasound on oxidative damage in retinal pigment epithelial cells in vitro. In: Ultrasound in Medicine and Biology. 2015 ; Vol. 41, No. 5. pp. 1363-1371.
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Effects of low-intensity ultrasound on oxidative damage in retinal pigment epithelial cells in vitro. / Kim, Na Kyeong; Kim, Chan Yun; Choi, Min Joo; Park, So Ra; Choi, Byung Hyune.

In: Ultrasound in Medicine and Biology, Vol. 41, No. 5, 01.05.2015, p. 1363-1371.

Research output: Contribution to journalArticle

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