Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(d,l-lactic-co-glycolic acid) scaffolds

Mi Hee Lee, Hye Lee Kim, Chang Hwa Kim, Sun Ho Lee, Jeong Koo Kim, Seung Jin Lee, Jong Chul Park

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) has been widely used as a scaffold in tissue engineering due to its excellent biocompatibility. However, PLGA scaffolds become structurally deformed during the sterilization process since they were hydrolytically unstable. In this study, the effects of hydrogen peroxide gas (HPG) were investigated on the deformation, sterilization and cytocompatibility of porous PLGA scaffolds and compared with those of ethylene oxide (EO) gas. Also, the effects of HPG treatment on the cellular responses of human articular chondrocytes were examined. HPG treatment resulted in neither deformation of PLGA nor its chemical modification, different from EO gas treatment. PLGA scaffolds inoculated with diverse strains of microorganisms were completely sterilized by HPG treatment. Moreover, the cytotoxicity of residual HPG occurring upon HPG treatment completely decreased as ventilation time increased. On the other hand, there was no significant difference in the proliferation and glycosaminoglycan synthesis of chondrocytes between EO gas-treated and HPG-treated PLGA scaffolds. These results suggest that HPG can be used as an alternative method for sterilizing various polymeric scaffolds and medical devices without structural deformation.

Original languageEnglish
Pages (from-to)5762-5767
Number of pages6
JournalSurface and Coatings Technology
Volume202
Issue number22-23
DOIs
Publication statusPublished - 2008 Aug 30

Bibliographical note

Funding Information:
This study was supported by the NanoBio R&D program [Platform technologies for organ/tissue regeneration (Regenomics), Grant No. 2005-00009] of the Korea Science and Engineering Foundation (KOSEF).

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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