Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(d,l-lactic-co-glycolic acid) scaffolds

Mi Hee Lee, Hye Lee Kim, Chang Hwa Kim, Sun Ho Lee, Jeong Koo Kim, Seung Jin Lee, Jong Chul Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) has been widely used as a scaffold in tissue engineering due to its excellent biocompatibility. However, PLGA scaffolds become structurally deformed during the sterilization process since they were hydrolytically unstable. In this study, the effects of hydrogen peroxide gas (HPG) were investigated on the deformation, sterilization and cytocompatibility of porous PLGA scaffolds and compared with those of ethylene oxide (EO) gas. Also, the effects of HPG treatment on the cellular responses of human articular chondrocytes were examined. HPG treatment resulted in neither deformation of PLGA nor its chemical modification, different from EO gas treatment. PLGA scaffolds inoculated with diverse strains of microorganisms were completely sterilized by HPG treatment. Moreover, the cytotoxicity of residual HPG occurring upon HPG treatment completely decreased as ventilation time increased. On the other hand, there was no significant difference in the proliferation and glycosaminoglycan synthesis of chondrocytes between EO gas-treated and HPG-treated PLGA scaffolds. These results suggest that HPG can be used as an alternative method for sterilizing various polymeric scaffolds and medical devices without structural deformation.

Original languageEnglish
Pages (from-to)5762-5767
Number of pages6
JournalSurface and Coatings Technology
Volume202
Issue number22-23
DOIs
Publication statusPublished - 2008 Aug 30

Fingerprint

glycolic acid
hydrogen peroxide
Scaffolds
Hydrogen peroxide
Hydrogen Peroxide
Gases
acids
Acids
gases
Ethylene Oxide
Temperature
Scaffolds (biology)
ethylene oxide
Ethylene
Oxides
Milk

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Lee, Mi Hee ; Kim, Hye Lee ; Kim, Chang Hwa ; Lee, Sun Ho ; Kim, Jeong Koo ; Lee, Seung Jin ; Park, Jong Chul. / Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(d,l-lactic-co-glycolic acid) scaffolds. In: Surface and Coatings Technology. 2008 ; Vol. 202, No. 22-23. pp. 5762-5767.
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Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(d,l-lactic-co-glycolic acid) scaffolds. / Lee, Mi Hee; Kim, Hye Lee; Kim, Chang Hwa; Lee, Sun Ho; Kim, Jeong Koo; Lee, Seung Jin; Park, Jong Chul.

In: Surface and Coatings Technology, Vol. 202, No. 22-23, 30.08.2008, p. 5762-5767.

Research output: Contribution to journalArticle

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