Effects of macelignan isolated from Myristica fragrans (nutmeg) on expression of matrix metalloproteinase-1 and type I procollagen in UVB-irradiated human skin fibroblasts

Kyung Eun Lee, Sukyeong Mun, Hee Bong Pyun, Myung Suk Kim, Jae Kwan Hwang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Exposure to ultraviolet (UV) light causes premature skin aging that is associated with upregulated matrix metalloproteinases (MMPs) and decreased collagen synthesis. Macelignan, a natural lignan compound isolated from Myristica fragrans HOUTT. (nutmeg), has been reported to possess antioxidant and anti-inflammatory activities. This study assessed the effects of macelignan on photoaging and investigated its mechanisms of action in UV-irradiated human skin fibroblasts (Hs68) by reverse transcription-polymerase chain reaction, Western blot analysis, 2′,7′-dichlorofluorescein diacetate assay, and enzyme-linked immunosorbent assay. Our results show that macelignan attenuated UV-induced MMP-1 expression by suppressing phosphorylation of mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Macelignan also increased type I procollagen expression and secretion through transforming growth factor β (TGF- β)/Smad signaling. These findings indicate that macelignan regulates the expression of MMP-1 and type I procollagen in UV-irradiated human skin fibroblasts by modulating MAPK and TGF-β/Smad signaling, suggesting its potential as an efficacious antiphotoaging agent.

Original languageEnglish
Pages (from-to)1669-1675
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume35
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

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Myristica fragrans
Matrix Metalloproteinase 1
Collagen Type I
Fibroblasts
Skin
Transforming Growth Factors
Mitogen-Activated Protein Kinases
Premature Aging
Skin Aging
Lignans
Ultraviolet Rays
Matrix Metalloproteinases
Reverse Transcription
Reactive Oxygen Species
Anti-Inflammatory Agents
Collagen
Antioxidants
Western Blotting
Enzyme-Linked Immunosorbent Assay
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

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title = "Effects of macelignan isolated from Myristica fragrans (nutmeg) on expression of matrix metalloproteinase-1 and type I procollagen in UVB-irradiated human skin fibroblasts",
abstract = "Exposure to ultraviolet (UV) light causes premature skin aging that is associated with upregulated matrix metalloproteinases (MMPs) and decreased collagen synthesis. Macelignan, a natural lignan compound isolated from Myristica fragrans HOUTT. (nutmeg), has been reported to possess antioxidant and anti-inflammatory activities. This study assessed the effects of macelignan on photoaging and investigated its mechanisms of action in UV-irradiated human skin fibroblasts (Hs68) by reverse transcription-polymerase chain reaction, Western blot analysis, 2′,7′-dichlorofluorescein diacetate assay, and enzyme-linked immunosorbent assay. Our results show that macelignan attenuated UV-induced MMP-1 expression by suppressing phosphorylation of mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Macelignan also increased type I procollagen expression and secretion through transforming growth factor β (TGF- β)/Smad signaling. These findings indicate that macelignan regulates the expression of MMP-1 and type I procollagen in UV-irradiated human skin fibroblasts by modulating MAPK and TGF-β/Smad signaling, suggesting its potential as an efficacious antiphotoaging agent.",
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Effects of macelignan isolated from Myristica fragrans (nutmeg) on expression of matrix metalloproteinase-1 and type I procollagen in UVB-irradiated human skin fibroblasts. / Lee, Kyung Eun; Mun, Sukyeong; Pyun, Hee Bong; Kim, Myung Suk; Hwang, Jae Kwan.

In: Biological and Pharmaceutical Bulletin, Vol. 35, No. 10, 01.10.2012, p. 1669-1675.

Research output: Contribution to journalArticle

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AU - Kim, Myung Suk

AU - Hwang, Jae Kwan

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