Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells

Eun Mi Choi; Jae Kwan Hwang

doi:10.1016/j.fitote.2003.11.003

Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells

Eun Mi Choi, Jae Kwan Hwang

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE₂ production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O₂^- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.

Original language	English
Pages (from-to)	141-148
Number of pages	8
Journal	Fitoterapia
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/j.fitote.2003.11.003
Publication status	Published - 2004 Mar

Bibliographical note

Funding Information:
The work was supported by the National Research Lab program through Functional Biopolymer Lab at Yonsei University (2000-N-NL-01-C-299) and the Korea Research Foundation Grant (KRF-2002-037-F00013).

All Science Journal Classification (ASJC) codes

Pharmacology
Drug Discovery

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10.1016/j.fitote.2003.11.003

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title = "Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells",

abstract = "Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.",

author = "Choi, {Eun Mi} and Hwang, {Jae Kwan}",

note = "Funding Information: The work was supported by the National Research Lab program through Functional Biopolymer Lab at Yonsei University (2000-N-NL-01-C-299) and the Korea Research Foundation Grant (KRF-2002-037-F00013).",

year = "2004",

month = mar,

doi = "10.1016/j.fitote.2003.11.003",

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AU - Choi, Eun Mi

AU - Hwang, Jae Kwan

N1 - Funding Information: The work was supported by the National Research Lab program through Functional Biopolymer Lab at Yonsei University (2000-N-NL-01-C-299) and the Korea Research Foundation Grant (KRF-2002-037-F00013).

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N2 - Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.

AB - Both the methanolic extract and its fractions (0.01 mg/ml) from Litsea cubeba bark were found to inhibit NO and PGE2 production in LPS-activated RAW264.7 macrophages without significant cytotoxicity at less than 0.01 mg/ml concentration. Moreover, L. cubeba bark extract and its fractions significantly decreased the activity of myeloperoxidase catalyzing oxidation of chloride to HOCl and O2- production was reduced by methanol extract (0.05 mg/ml), chloroform fraction (0.025-0.05 mg/ml) and butanol fraction (0.005 mg/ml). These findings suggest that L. cubeba is beneficial for inflammatory conditions and may contain compound(s) with anti-inflammatory properties.

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Effects of methanolic extract and fractions from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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