Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages

Eun Mi Choi; Jae Kwan Hwang

doi:10.1016/j.fitote.2005.05.006

Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E₂ and cytokines in RAW264.7 macrophages

Eun Mi Choi, Jae Kwan Hwang

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Morus alba leaf methanolic extract and its fractions (chloroform, butanol, and aqueous fractions) were found to inhibit NO production in LPS-activated RAW264.7 macrophages without an appreciable cytotoxic effect at concentration from 4 to 100 μg/ml. LPS-induced PGE₂ production was significantly reduced only by butanol fraction. In addition, M. alba leaf extract and its fractions significantly decreased the production of TNF-α. These findings suggest that M. alba leaf extract seems to be able in suppressing inflammatory mediators. Moreover, the inhibitory activities on COX-2 and iNOS of its butanol fraction are warranted for further elucidation of active principles for development of new antiinflammatory agents.

Original language	English
Pages (from-to)	608-613
Number of pages	6
Journal	Fitoterapia
Volume	76
Issue number	7-8
DOIs	https://doi.org/10.1016/j.fitote.2005.05.006
Publication status	Published - 2005 Dec

Bibliographical note

Funding Information:
The work was supported by the Korea Research Foundation Grant (KRF-2002-037-F00013).

All Science Journal Classification (ASJC) codes

Pharmacology
Drug Discovery

Access to Document

10.1016/j.fitote.2005.05.006

Cite this

@article{3a60031bb72f4870bf2096a95a4eaaa5,

title = "Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages",

abstract = "Morus alba leaf methanolic extract and its fractions (chloroform, butanol, and aqueous fractions) were found to inhibit NO production in LPS-activated RAW264.7 macrophages without an appreciable cytotoxic effect at concentration from 4 to 100 μg/ml. LPS-induced PGE2 production was significantly reduced only by butanol fraction. In addition, M. alba leaf extract and its fractions significantly decreased the production of TNF-α. These findings suggest that M. alba leaf extract seems to be able in suppressing inflammatory mediators. Moreover, the inhibitory activities on COX-2 and iNOS of its butanol fraction are warranted for further elucidation of active principles for development of new antiinflammatory agents.",

author = "Choi, {Eun Mi} and Hwang, {Jae Kwan}",

note = "Funding Information: The work was supported by the Korea Research Foundation Grant (KRF-2002-037-F00013).",

year = "2005",

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T1 - Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages

AU - Choi, Eun Mi

AU - Hwang, Jae Kwan

N1 - Funding Information: The work was supported by the Korea Research Foundation Grant (KRF-2002-037-F00013).

PY - 2005/12

Y1 - 2005/12

N2 - Morus alba leaf methanolic extract and its fractions (chloroform, butanol, and aqueous fractions) were found to inhibit NO production in LPS-activated RAW264.7 macrophages without an appreciable cytotoxic effect at concentration from 4 to 100 μg/ml. LPS-induced PGE2 production was significantly reduced only by butanol fraction. In addition, M. alba leaf extract and its fractions significantly decreased the production of TNF-α. These findings suggest that M. alba leaf extract seems to be able in suppressing inflammatory mediators. Moreover, the inhibitory activities on COX-2 and iNOS of its butanol fraction are warranted for further elucidation of active principles for development of new antiinflammatory agents.

AB - Morus alba leaf methanolic extract and its fractions (chloroform, butanol, and aqueous fractions) were found to inhibit NO production in LPS-activated RAW264.7 macrophages without an appreciable cytotoxic effect at concentration from 4 to 100 μg/ml. LPS-induced PGE2 production was significantly reduced only by butanol fraction. In addition, M. alba leaf extract and its fractions significantly decreased the production of TNF-α. These findings suggest that M. alba leaf extract seems to be able in suppressing inflammatory mediators. Moreover, the inhibitory activities on COX-2 and iNOS of its butanol fraction are warranted for further elucidation of active principles for development of new antiinflammatory agents.

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