Effects of Mycophenolic Acid on High Glucose-Induced Fibronectin Secretion and Cellular Reactive Oxygen Species in Rat Vascular Smooth Muscle Cells

J. Park, M. K. Ju, J. S. Song, K. H. Huh, M. S. Kim, YuSeun Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Vascular smooth muscle cell (VSMC) proliferation, migration, and matrix protein accumulation play important roles in the development and progression of vascular disease including diabetic vascular complications and chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation and matrix protein accumulation and reactive oxygen species (ROS). In this study, we investigated the effects of MPA on high glucose (HG)-induced fibronectin secretion and the role of ROS in rat VSMCs. Primary cultured rat VSMCs from Sprague-Dawley rats were exposed for 1 hour before stimulation with media containing 5.6 mmol/L glucose (low glucose [LG]), 30 mmol/L mannitol (M), or 30 mmol/L glucose (HG) with or without MPA (0.1-10 μmol/L) or N-acetylcysteine (NAC; 5 mmol/L). Fibronectin secretion was measured by Western blot analysis and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. HG significantly increased fibronectin secretion by 1.7-fold. The increment of DCF-sensitive cellular ROS was 1.5-fold at 1 hour by HG. MPA at concentrations above 1 μmol/L effectively inhibited HG-induced fibronectin secretion and cellular ROS in a dose-dependent manner. NAC at 5 mmol/L also inhibited HG-induced rat VSMC activation. These results suggested that MPA inhibits HG-induced VSMC activation partially through inhibiting cellular ROS.

Original languageEnglish
Pages (from-to)467-469
Number of pages3
JournalTransplantation Proceedings
Volume40
Issue number2
DOIs
Publication statusPublished - 2008 Mar 1

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Mycophenolic Acid
Vascular Smooth Muscle
Fibronectins
Smooth Muscle Myocytes
Reactive Oxygen Species
Glucose
Cell Proliferation
Diabetic Angiopathies
Acetylcysteine
Mannitol
Vascular Diseases
Cell Movement
Allografts
Sprague Dawley Rats
Flow Cytometry
Proteins
Western Blotting

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

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abstract = "Vascular smooth muscle cell (VSMC) proliferation, migration, and matrix protein accumulation play important roles in the development and progression of vascular disease including diabetic vascular complications and chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation and matrix protein accumulation and reactive oxygen species (ROS). In this study, we investigated the effects of MPA on high glucose (HG)-induced fibronectin secretion and the role of ROS in rat VSMCs. Primary cultured rat VSMCs from Sprague-Dawley rats were exposed for 1 hour before stimulation with media containing 5.6 mmol/L glucose (low glucose [LG]), 30 mmol/L mannitol (M), or 30 mmol/L glucose (HG) with or without MPA (0.1-10 μmol/L) or N-acetylcysteine (NAC; 5 mmol/L). Fibronectin secretion was measured by Western blot analysis and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. HG significantly increased fibronectin secretion by 1.7-fold. The increment of DCF-sensitive cellular ROS was 1.5-fold at 1 hour by HG. MPA at concentrations above 1 μmol/L effectively inhibited HG-induced fibronectin secretion and cellular ROS in a dose-dependent manner. NAC at 5 mmol/L also inhibited HG-induced rat VSMC activation. These results suggested that MPA inhibits HG-induced VSMC activation partially through inhibiting cellular ROS.",
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Effects of Mycophenolic Acid on High Glucose-Induced Fibronectin Secretion and Cellular Reactive Oxygen Species in Rat Vascular Smooth Muscle Cells. / Park, J.; Ju, M. K.; Song, J. S.; Huh, K. H.; Kim, M. S.; Kim, YuSeun.

In: Transplantation Proceedings, Vol. 40, No. 2, 01.03.2008, p. 467-469.

Research output: Contribution to journalArticle

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